QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (105) Citing Articles via Google Scholar Google Scholar Articles by Rasband, M. N. Articles by Shrager, P. Search for Related Content PubMed PubMed Citation Articles by Rasband, M. N. Articles by Shrager, P.

 Previous Article  |  Next Article 

The Journal of Neuroscience, January 1, 1998, 18(1):36-47

Potassium Channel Distribution, Clustering, and Function in Remyelinating Rat Axons

Matthew N. Rasband¹, James S. Trimmer³, Thomas L. Schwarz⁴, S. Rock Levinson⁵, Mark H. Ellisman⁶, Melitta Schachner⁷, and Peter Shrager²

Departments of ¹ Biochemistry and Biophysics and ² Neurobiology and Anatomy, University of Rochester Medical Center, Rochester, New York 14642, ³ Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794, ⁴ Department of Molecular and Cellular Physiology, Beckman Center, Stanford University, Stanford, California 94305, ⁵ Health Sciences Center, University of Colorado, Denver, Colorado 80262, ⁶ Department of Neurosciences, University of California San Diego, La Jolla, California 92093-0608, and ⁷ Zentrum fur Moleculare Neurobiologie, Universitat Hamburg, Hamburg, Germany D-20246

The K⁺ channel -subunits Kv1.1 and Kv1.2 and the cytoplasmic -subunit Kv2 were detected by immunofluorescence microscopy and found to be colocalized at juxtaparanodes in normal adult rat sciatic nerve. After demyelination by intraneural injection of lysolecithin, and during remyelination, the subcellular distributions of Kv1.1, Kv1.2, and Kv2 were reorganized. At 6 d postinjection (dpi), axons were stripped of myelin, and K⁺ channels were found to be dispersed across zones that extended into both nodal and internodal regions; a few days later they were undetectable. By 10 dpi, remyelination was underway, but Kv1.1 immunoreactivity was absent at newly forming nodes of Ranvier. By 14 dpi, K⁺ channels were detected but were in the nodal gap between Schwann cells. By 19 dpi, most new nodes had Kv1.1, Kv1.2, and Kv2, which precisely colocalized. However, this nodal distribution was transient. By 24 dpi, the majority of K⁺ channels was clustered within paranodal regions of remyelinated axons, leaving a gap that overlapped with Na⁺ channel immunoreactivity. Inhibition of Schwann cell proliferation delayed both remyelination and the development of the K⁺ channel distributions described. Conduction studies indicate that neither 4-aminopyridine (4-AP) nor tetraethylammonium alters normal nerve conduction. However, during remyelination, 4-AP profoundly increased both compound action potential amplitude and duration. The level of this effect matched closely the nodal presence of these voltage-dependent K⁺ channels. Our results suggest that K⁺ channels may have a significant effect on conduction during remyelination and that Schwann cells are important in K⁺ channel redistribution and clustering.

Key words: potassium channels; demyelination; remyelination; Schwann cells; axons; node of Ranvier

---

Copyright © 1998 Society for Neuroscience  0270-6474/98/18136-12$05.00/0

This article has been cited by other articles:

				M. N. Miller, B. W. Okaty, and S. B. Nelson Region-Specific Spike-Frequency Acceleration in Layer 5 Pyramidal Neurons Mediated by Kv1 Subunits J. Neurosci., December 17, 2008; 28(51): 13716 - 13726. [Abstract] [Full Text] [PDF]

				H. Vacher, D. P. Mohapatra, and J. S. Trimmer Localization and Targeting of Voltage-Dependent Ion Channels in Mammalian Central Neurons Physiol Rev, October 1, 2008; 88(4): 1407 - 1447. [Abstract] [Full Text] [PDF]

				J. Biernaskie, J. S. Sparling, J. Liu, C. P. Shannon, J. R. Plemel, Y. Xie, F. D. Miller, and W. Tetzlaff Skin-Derived Precursors Generate Myelinating Schwann Cells That Promote Remyelination and Functional Recovery after Contusion Spinal Cord Injury J. Neurosci., September 5, 2007; 27(36): 9545 - 9559. [Abstract] [Full Text] [PDF]

				E. Eftekharpour, S. Karimi-Abdolrezaee, J. Wang, H. El Beheiry, C. Morshead, and M. G. Fehlings Myelination of Congenitally Dysmyelinated Spinal Cord Axons by Adult Neural Precursor Cells Results in Formation of Nodes of Ranvier and Improved Axonal Conduction J. Neurosci., March 28, 2007; 27(13): 3416 - 3428. [Abstract] [Full Text] [PDF]

				Y. Bai, E. Ianokova, Q. Pu, K. Ghandour, R. Levinson, J.-J. Martin, C. Ceuterick-de Groote, R. Mazanec, P. Seeman, M. E. Shy, et al. Effect of an R69C Mutation in the Myelin Protein Zero Gene on Myelination and Ion Channel Subtypes Arch Neurol, December 1, 2006; 63(12): 1787 - 1794. [Abstract] [Full Text] [PDF]

				I. Coman, M. S. Aigrot, D. Seilhean, R. Reynolds, J. A. Girault, B. Zalc, and C. Lubetzki Nodal, paranodal and juxtaparanodal axonal proteins during demyelination and remyelination in multiple sclerosis Brain, December 1, 2006; 129(12): 3186 - 3195. [Abstract] [Full Text] [PDF]

				O. W. Howell, A. Palser, A. Polito, S. Melrose, B. Zonta, C. Scheiermann, A. J. Vora, P. J. Brophy, and R. Reynolds Disruption of neurofascin localization reveals early changes preceding demyelination and remyelination in multiple sclerosis Brain, December 1, 2006; 129(12): 3173 - 3185. [Abstract] [Full Text] [PDF]

				K. A. Kleopa, L. B. Elman, B. Lang, A. Vincent, and S. S. Scherer Neuromyotonia and limbic encephalitis sera target mature Shaker-type K+ channels: subunit specificity correlates with clinical manifestations Brain, June 1, 2006; 129(6): 1570 - 1584. [Abstract] [Full Text] [PDF]

				J. R. Schwarz, G. Glassmeier, E. C. Cooper, T.-C Kao, H. Nodera, D. Tabuena, R. Kaji, and H. Bostock KCNQ channels mediate IKs, a slow K+ current regulating excitability in the rat node of Ranvier J. Physiol., May 15, 2006; 573(1): 17 - 34. [Abstract] [Full Text] [PDF]

				Z. Pan, T. Kao, Z. Horvath, J. Lemos, J.-Y. Sul, S. D. Cranstoun, V. Bennett, S. S. Scherer, and E. C. Cooper A common ankyrin-G-based mechanism retains KCNQ and NaV channels at electrically active domains of the axon. J. Neurosci., March 8, 2006; 26(10): 2599 - 2613. [Abstract] [Full Text] [PDF]

				K. Sinha, S. Karimi-Abdolrezaee, A. A. Velumian, and M. G. Fehlings Functional Changes in Genetically Dysmyelinated Spinal Cord Axons of Shiverer Mice: Role of Juxtaparanodal Kv1 Family K+ Channels J Neurophysiol, March 1, 2006; 95(3): 1683 - 1695. [Abstract] [Full Text] [PDF]

				M. Sasaki, J. A. Black, K. L. Lankford, H. A. Tokuno, S. G. Waxman, and J. D. Kocsis Molecular Reconstruction of Nodes of Ranvier after Remyelination by Transplanted Olfactory Ensheathing Cells in the Demyelinated Spinal Cord J. Neurosci., February 8, 2006; 26(6): 1803 - 1812. [Abstract] [Full Text] [PDF]

				J. J. Devaux and S. S. Scherer Altered Ion Channels in an Animal Model of Charcot-Marie-Tooth Disease Type IA J. Neurosci., February 9, 2005; 25(6): 1470 - 1480. [Abstract] [Full Text] [PDF]

				T. Marqueste, J.-R. Alliez, O. Alluin, Y. Jammes, and P. Decherchi Neuromuscular rehabilitation by treadmill running or electrical stimulation after peripheral nerve injury and repair J Appl Physiol, May 1, 2004; 96(5): 1988 - 1995. [Abstract] [Full Text] [PDF]

				W. Shen, S. Hernandez-Lopez, T. Tkatch, J. E. Held, and D. J. Surmeier Kv1.2-Containing K+ Channels Regulate Subthreshold Excitability of Striatal Medium Spiny Neurons J Neurophysiol, March 1, 2004; 91(3): 1337 - 1349. [Abstract] [Full Text] [PDF]

				T. Ishibashi, L. Ding, K. Ikenaka, Y. Inoue, K. Miyado, E. Mekada, and H. Baba Tetraspanin Protein CD9 Is a Novel Paranodal Component Regulating Paranodal Junctional Formation J. Neurosci., January 7, 2004; 24(1): 96 - 102. [Abstract] [Full Text] [PDF]

				S. E. Hanak, E. M. Reilly, J. Wotanis, B. Zhu, C. Pulicicchio, K. McMonagle-Strucko, J. G. Wettstein, and M. D. Black An Electrophysiological Model of Spinal Transmission Deficits in Mouse Experimental Autoimmune Encephalomyelitis J. Pharmacol. Exp. Ther., January 1, 2004; 308(1): 214 - 220. [Abstract] [Full Text] [PDF]

				S. Poliak, D. Salomon, H. Elhanany, H. Sabanay, B. Kiernan, L. Pevny, C. L. Stewart, X. Xu, S.-Y. Chiu, P. Shrager, et al. Juxtaparanodal clustering of Shaker-like K+ channels in myelinated axons depends on Caspr2 and TAG-1 J. Cell Biol., September 15, 2003; 162(6): 1149 - 1160. [Abstract] [Full Text] [PDF]

				M. N. Rasband, E. W. Park, D. Zhen, M. I. Arbuckle, S. Poliak, E. Peles, S. G.N. Grant, and J. S. Trimmer Clustering of neuronal potassium channels is independent of their interaction with PSD-95 J. Cell Biol., November 25, 2002; 159(4): 663 - 672. [Abstract] [Full Text] [PDF]

				Y. Xu, N. Chiamvimonvat, A. E. Vazquez, S. Akunuru, N. Ratner, and E. N. Yamoah Gene-Targeted Deletion of Neurofibromin Enhances the Expression of a Transient Outward K+ Current in Schwann Cells: A Protein Kinase A-Mediated Mechanism J. Neurosci., November 1, 2002; 22(21): 9194 - 9202. [Abstract] [Full Text] [PDF]

				S. Akhtar, O. Shamotienko, M. Papakosta, F. Ali, and J. O. Dolly Characteristics of Brain Kv1 Channels Tailored to Mimic Native Counterparts by Tandem Linkage of alpha Subunits. IMPLICATIONS FOR K+ CHANNELOPATHIES J. Biol. Chem., May 3, 2002; 277(19): 16376 - 16382. [Abstract] [Full Text] [PDF]

				J. Devaux, M. Gola, G. Jacquet, and M. Crest Effects of K+ Channel Blockers on Developing Rat Myelinated CNS Axons: Identification of Four Types of K+ Channels J Neurophysiol, March 1, 2002; 87(3): 1376 - 1385. [Abstract] [Full Text] [PDF]

				E. J. Arroyo, T. Xu, J. Grinspan, S. Lambert, S. R. Levinson, P. J. Brophy, E. Peles, and S. S. Scherer Genetic Dysmyelination Alters the Molecular Architecture of the Nodal Region J. Neurosci., March 1, 2002; 22(5): 1726 - 1737. [Abstract] [Full Text] [PDF]

				M. N. Rasband, E. W. Park, T. W. Vanderah, J. Lai, F. Porreca, and J. S. Trimmer Distinct potassium channels on pain-sensing neurons PNAS, November 6, 2001; 98(23): 13373 - 13378. [Abstract] [Full Text] [PDF]

				S. Martin, A. K. Levine, Z. J. Chen, Y. Ughrin, and J. M. Levine Deposition of the NG2 Proteoglycan at Nodes of Ranvier in the Peripheral Nervous System J. Neurosci., October 15, 2001; 21(20): 8119 - 8128. [Abstract] [Full Text] [PDF]

				C. Beeton, J. Barbaria, P. Giraud, J. Devaux, A.-M. Benoliel, M. Gola, J. M. Sabatier, D. Bernard, M. Crest, and E. Beraud Selective Blocking of Voltage-Gated K+ Channels Improves Experimental Autoimmune Encephalomyelitis and Inhibits T Cell Activation J. Immunol., January 15, 2001; 166(2): 936 - 944. [Abstract] [Full Text] [PDF]

				T. Tkatch, G. Baranauskas, and D. J. Surmeier Kv4.2 mRNA Abundance and A-Type K+ Current Amplitude Are Linearly Related in Basal Ganglia and Basal Forebrain Neurons J. Neurosci., January 15, 2000; 20(2): 579 - 588. [Abstract] [Full Text] [PDF]

				D. G. M. Jugloff, R. Khanna, L. C. Schlichter, and O. T. Jones Internalization of the Kv1.4 Potassium Channel Is Suppressed by Clustering Interactions with PSD-95 J. Biol. Chem., January 14, 2000; 275(2): 1357 - 1364. [Abstract] [Full Text] [PDF]

				A. M. Tiffany, L. N. Manganas, E. Kim, Y.-P. Hsueh, M. Sheng, and J. S. Trimmer PSD-95 and SAP97 Exhibit Distinct Mechanisms for Regulating K+ Channel Surface Expression and Clustering J. Cell Biol., January 10, 2000; 148(1): 147 - 158. [Abstract] [Full Text] [PDF]

				M. N. Rasband, E. Peles, J. S. Trimmer, S. R. Levinson, S. E. Lux, and P. Shrager Dependence of Nodal Sodium Channel Clustering on Paranodal Axoglial Contact in the Developing CNS J. Neurosci., September 1, 1999; 19(17): 7516 - 7528. [Abstract] [Full Text] [PDF]

				P. Weber, U. Bartsch, M. N. Rasband, R. Czaniera, Y. Lang, H. Bluethmann, R. U. Margolis, S. R. Levinson, P. Shrager, D. Montag, et al. Mice Deficient for Tenascin-R Display Alterations of the Extracellular Matrix and Decreased Axonal Conduction Velocities in the CNS J. Neurosci., June 1, 1999; 19(11): 4245 - 4262. [Abstract] [Full Text] [PDF]

				I. Vabnick, J. S. Trimmer, T. L. Schwarz, S. R. Levinson, D. Risal, and P. Shrager Dynamic Potassium Channel Distributions during Axonal Development Prevent Aberrant Firing Patterns J. Neurosci., January 15, 1999; 19(2): 747 - 758. [Abstract] [Full Text] [PDF]

				A. Sobko, A. Peretz, O. Shirihai, S. Etkin, V. Cherepanova, D. Dagan, and B. Attali Heteromultimeric Delayed-Rectifier K+ Channels in Schwann Cells: Developmental Expression and Role in Cell Proliferation J. Neurosci., December 15, 1998; 18(24): 10398 - 10408. [Abstract] [Full Text] [PDF]

				L. Zhou, C.-L. Zhang, A. Messing, and S. Y. Chiu Temperature-Sensitive Neuromuscular Transmission in Kv1.1 Null Mice: Role of Potassium Channels under the Myelin Sheath in Young Nerves J. Neurosci., September 15, 1998; 18(18): 7200 - 7215. [Abstract] [Full Text] [PDF]

				L. N. Manganas, S. Akhtar, D. E. Antonucci, C. R. Campomanes, J. O. Dolly, and J. S. Trimmer Episodic Ataxia Type-1 Mutations in the Kv1.1 Potassium Channel Display Distinct Folding and Intracellular Trafficking Properties J. Biol. Chem., December 21, 2001; 276(52): 49427 - 49434. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help