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The Journal of Neuroscience, January 1, 1998, 18(1):59-69

Developmental Regulation of the cm2 Muscarinic Acetylcholine Receptor Gene: Selective Induction by a Secreted Factor Produced by Embryonic Chick Retinal Cells

Lise A. McKinnon¹, Erik C. Gunther², and Neil M. Nathanson¹

¹ Department of Pharmacology, University of Washington School of Medicine, Seattle, Washington, 98195-7750, and ² Department of Physiology and Biophysics, Molecular and Cellular Biology Program, University of Washington School of Medicine, Seattle, Washington, 98195-7290

The expression of the cm2 muscarinic acetylcholine receptor gene increases dramatically in chick retina during embryonic development in vivo. A similar developmental increase in cm2 expression occurs in embryonic chick retinal cells in culture. Conditioned medium from mature, but not young, retinal cultures contains a secreted factor that causes a selective increase in expression of cm2, but not cm3 or cm4, receptors. The secreted factor has been partially purified from serum-free medium, is protease-sensitive, and has a molecular weight >10 kDa. The cm2-inducing factor stimulates expression of a cm2 promoter/luciferase reporter gene, demonstrating that the increase in cm2 expression is attributable to increased gene transcription. Incubation of retinal cells with 14 identified neurotrophic and growth factors did not increase cm2 expression, suggesting that a novel developmentally regulated secreted factor mediates the subtype-specific induction of the cm2 receptor gene in retina.

Key words: gene regulation; mAChR; retina; embryonic development; subtype selectivity; secreted factor

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Copyright © 1998 Society for Neuroscience  0270-6474/98/18159-11$05.00/0

This article has been cited by other articles:

				K. E. Belmonte, L. A. McKinnon, and N. M. Nathanson Developmental Expression of Muscarinic Acetylcholine Receptors in Chick Retina: Selective Induction of M2 Muscarinic Receptor Expression In Ovo by a Factor Secreted by Muller Glial Cells J. Neurosci., November 15, 2000; 20(22): 8417 - 8425. [Abstract] [Full Text] [PDF]

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