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The Journal of Neuroscience, June 1, 1998, 18(11):4393-4402

Activation of Coeruleospinal Noradrenergic Inhibitory Controls during Withdrawal from Morphine in the Rat

Dana S. Rohde^{2, 3} and Allan I. Basbaum^{1, 2, 3}

Program in Biomedical Sciences, Departments of ¹ Anatomy and ² Physiology and ³ W. M. Keck Foundation Center for Integrative Neuroscience, University of California at San Francisco, San Francisco, California 94143

We previously reported that withdrawal from morphine induces the expression of Fos, a marker of neuronal activity, in spinal cord neurons, particularly in laminae I and II of the superficial dorsal horn, and that the magnitude of Fos expression is increased in rats with a midthoracic spinal transection. We suggested that loss of withdrawal-associated increases in descending inhibitory controls that arise in the brainstem underlie the increased Fos expression after spinal transection. Here, we addressed the origin of the supraspinal inhibition. We injected rats intracerebroventricularly with saline or anti-dopamine--hydroxylase-saporin, a toxin that destroys noradrenergic neurons of the locus coeruleus. Eleven days later, we implanted rats with morphine or placebo pellets, and after 4 d, we precipitated withdrawal with naltrexone. One hour later, the rats were killed, their brains and spinal cords were removed, and transverse sections of the brains and spinal cords were immunoreacted with an antibody to Fos.

In placebo-pelleted rats, the toxin injection did not alter behavior and did not induce expression of the Fos protein. However, compared with saline-injected withdrawing rats, the toxin-treated rats that underwent withdrawal demonstrated an intense withdrawal behavior rarely seen in the absence of toxin, namely forepaw fluttering. The rats also had significantly increased Fos-like immunoreactivity in all laminae of the cervical cord and in laminae I and II and the ventral horn of the lumbar cord. No differences were recorded in the sacral cord. We conclude that the effects of spinal transection in rats that withdraw from morphine in part reflect a loss of coeruleospinal noradrenergic inhibitory controls.

Key words: descending inhibition; Fos; locus coeruleus; morphine withdrawal; saporin; spinal cord; tolerance

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Copyright © 1998 Society for Neuroscience  0270-6474/98/18114393-10$05.00/0

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