 |
||||
|
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, June 15, 1998, 18(12):4500-4510
Excitatory Synaptic Transmission in the Inner Retina: Paired Recordings of Bipolar Cells and Neurons of the Ganglion Cell Layer
Ko Matsui , Nobutake Hosoi , and Masao Tachibana Department of Psychology, Graduate School of Humanities and Sociology, The University of Tokyo, Tokyo 113-0033, Japan
Properties of glutamatergic synaptic transmission were investigated by simultaneously voltage-clamping a pair of connected bipolar cells and cells in the ganglion cell layer (GLCs) in the newt retinal slice preparation. Activation of the Ca2+ current in a single bipolar cell was essential for evoking the glutamatergic postsynaptic current in the GLC. Depolarization for as short as 15 msec activated both NMDA and non-NMDA receptors. On the other hand, analysis of the spontaneous glutamatergic synaptic currents of GLCs revealed that these currents consisted of mainly non-NMDA receptor activation with little contribution from NMDA receptors. This suggests that non-NMDA receptors of GLCs are clustered in postsynaptic membrane regions immediately beneath the release sites of bipolar cells and that NMDA receptors have lower accessibility to the released transmitter than non-NMDA receptors. Glutamate that is spilled over from the release sites may activate the NMDA receptors. When a prolonged depolarizing pulse was applied to a bipolar cell, the response induced by non-NMDA receptors was limited greatly by their fast desensitization, whereas NMDA receptors were able to produce a maintained response. The relationship between the pulse duration applied to the bipolar cell and the integrated charge of the response evoked in the GLC was almost linear. Therefore, we propose that both non-NMDA and NMDA receptors cooperate to transfer the graded photoresponses of bipolar cells proportionally to GLCs.
Key words: retina; synaptic transmission; glutamate; non-NMDA receptor; NMDA receptor; EPSC; spontaneous EPSC; bipolar cell; ganglion cell; desensitization; spill-over
Copyright © 1998 Society for Neuroscience 0270-6474/98/18124500-11$05.00/0
This article has been cited by other articles:
J. Zhang and J. S. Diamond
Subunit- and Pathway-Specific Localization of NMDA Receptors and Scaffolding Proteins at Ganglion Cell Synapses in Rat Retina
J. Neurosci., April 1, 2009; 29(13): 4274 - 4286.
[Abstract] [Full Text] [PDF]
T. L. Kalbaugh, J. Zhang, and J. S. Diamond
Coagonist Release Modulates NMDA Receptor Subtype Contributions at Synaptic Inputs to Retinal Ganglion Cells
J. Neurosci., February 4, 2009; 29(5): 1469 - 1479.
[Abstract] [Full Text] [PDF]
E. Margalit and W. B. Thoreson
Inner retinal mechanisms engaged by retinal electrical stimulation.
Invest. Ophthalmol. Vis. Sci., June 1, 2006; 47(6): 2606 - 2612.
[Abstract] [Full Text] [PDF]
Y. Xia, R. C. Carroll, and S. Nawy
State-dependent AMPA receptor trafficking in the Mammalian retina.
J. Neurosci., May 10, 2006; 26(19): 5028 - 5036.
[Abstract] [Full Text] [PDF]
L. Cadetti, D. Tranchina, and W. B. Thoreson
A comparison of release kinetics and glutamate receptor properties in shaping rod-cone differences in EPSC kinetics in the salamander retina
J. Physiol., December 15, 2005; 569(3): 773 - 788.
[Abstract] [Full Text] [PDF]
N. Hosoi, I. Arai, and M. Tachibana
Group III Metabotropic Glutamate Receptors and Exocytosed Protons Inhibit L-Type Calcium Currents in Cones But Not in Rods
J. Neurosci., April 20, 2005; 25(16): 4062 - 4072.
[Abstract] [Full Text] [PDF]
P. Koulen, J. Wei, C. Madry, J. Liu, and E. Nixon
Differentially Distributed IP3 Receptors and Ca2+ Signaling in Rod Bipolar Cells
Invest. Ophthalmol. Vis. Sci., January 1, 2005; 46(1): 292 - 298.
[Abstract] [Full Text] [PDF]
E. Garbarino-Pico, A. R. Carpentieri, M. A. Contin, M. I. K. Sarmiento, M. A. Brocco, P. Panzetta, R. E. Rosenstein, B. L. Caputto, and M. E. Guido
Retinal Ganglion Cells Are Autonomous Circadian Oscillators Synthesizing N-Acetylserotonin during the Day
J. Biol. Chem., December 3, 2004; 279(49): 51172 - 51181.
[Abstract] [Full Text] [PDF]
D. Zenisek, V. Davila, L. Wan, and W. Almers
Imaging Calcium Entry Sites and Ribbon Structures in Two Presynaptic Cells
J. Neurosci., April 1, 2003; 23(7): 2538 - 2548.
[Abstract] [Full Text] [PDF]
M. H. Hennig, K. Funke, and F. Worgotter
The Influence of Different Retinal Subcircuits on the Nonlinearity of Ganglion Cell Behavior
J. Neurosci., October 1, 2002; 22(19): 8726 - 8738.
[Abstract] [Full Text] [PDF]
Y. Ikegaya, S. Matsuura, S. Ueno, A. Baba, M. K. Yamada, N. Nishiyama, and N. Matsuki
beta -Amyloid Enhances Glial Glutamate Uptake Activity and Attenuates Synaptic Efficacy
J. Biol. Chem., August 23, 2002; 277(35): 32180 - 32186.
[Abstract] [Full Text] [PDF]
T. Ichinose and P. D. Lukasiewicz
GABA Transporters Regulate Inhibition in the Retina by Limiting GABAC Receptor Activation
J. Neurosci., April 15, 2002; 22(8): 3285 - 3292.
[Abstract] [Full Text] [PDF]
S. Chen and J. S. Diamond
Synaptically Released Glutamate Activates Extrasynaptic NMDA Receptors on Cells in the Ganglion Cell Layer of Rat Retina
J. Neurosci., March 15, 2002; 22(6): 2165 - 2173.
[Abstract] [Full Text] [PDF]
E. Neher and T. Sakaba
Estimating Transmitter Release Rates from Postsynaptic Current Fluctuations
J. Neurosci., December 15, 2001; 21(24): 9638 - 9654.
[Abstract] [Full Text] [PDF]
K. Matsui, J. Hasegawa, and M. Tachibana
Modulation of Excitatory Synaptic Transmission by GABAC Receptor-Mediated Feedback in the Mouse Inner Retina
J Neurophysiol, November 1, 2001; 86(5): 2285 - 2298.
[Abstract] [Full Text] [PDF]
M. J. Frech, J. Perez-Leon, H. Wassle, and K. H. Backus
Characterization of the Spontaneous Synaptic Activity of Amacrine Cells in the Mouse Retina
J Neurophysiol, October 1, 2001; 86(4): 1632 - 1643.
[Abstract] [Full Text] [PDF]
E. D. Cohen
Light-Evoked Excitatory Synaptic Currents of X-Type Retinal Ganglion Cells
J Neurophysiol, June 1, 2000; 83(6): 3217 - 3229.
[Abstract] [Full Text] [PDF]
M. A. Freed
Parallel Cone Bipolar Pathways to a Ganglion Cell Use Different Rates and Amplitudes of Quantal Excitation
J. Neurosci., June 1, 2000; 20(11): 3956 - 3963.
[Abstract] [Full Text] [PDF]
J. Burrone and L. Lagnado
Synaptic Depression and the Kinetics of Exocytosis in Retinal Bipolar Cells
J. Neurosci., January 15, 2000; 20(2): 568 - 578.
[Abstract] [Full Text] [PDF]
K. Matsui, N. Hosoi, and M. Tachibana
Active Role of Glutamate Uptake in the Synaptic Transmission from Retinal Nonspiking Neurons
J. Neurosci., August 15, 1999; 19(16): 6755 - 6766.
[Abstract] [Full Text] [PDF]
E. Hartveit
Reciprocal Synaptic Interactions Between Rod Bipolar Cells and Amacrine Cells in the Rat Retina
J Neurophysiol, June 1, 1999; 81(6): 2923 - 2936.
[Abstract] [Full Text] [PDF]
M. H. Higgs and P. D. Lukasiewicz
Glutamate Uptake Limits Synaptic Excitation of Retinal Ganglion Cells
J. Neurosci., May 15, 1999; 19(10): 3691 - 3700.
[Abstract] [Full Text] [PDF]
F. Kawai and P. Sterling
AMPA Receptor Activates a G-Protein that Suppresses a cGMP-Gated Current
J. Neurosci., April 15, 1999; 19(8): 2954 - 2959.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|