QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (37) Citing Articles via Google Scholar Google Scholar Articles by Pierre, K. Articles by Theodosis, Dionysia. T. Search for Related Content PubMed PubMed Citation Articles by Pierre, K. Articles by Theodosis, Dionysia. T.

 Previous Article  |  Next Article 

The Journal of Neuroscience, July 15, 1998, 18(14):5333-5343

Regulated Expression of the Cell Adhesion Glycoprotein F3 in Adult Hypothalamic Magnocellular Neurons

Karin Pierre¹, Geneviève Rougon², Michèle Allard¹, Renée Bonhomme¹, Gianfranco Gennarini³, Dominique A. Poulain¹, and Dionysia. T. Theodosis¹

¹ Institut National de la Santé et de la Recherche Médicale U378 Neurobiologie Morphofonctionelle, Institut François Magendie, F33077 Bordeaux Cedex, France, ² Centre National de la Recherche Scientifique UMR 9943 Laboratoire de Génétique et Physiologie du Développement, Parc Scientifique de Luminy, F13288 Marseille Cedex 9, France, and ³ Dipartimento di Farmacologia e Fisiologia Umana, University of Bari, Bari, I 702124 Italy

F3, a glycoprotein of the immunoglobulin superfamily implicated in axonal growth, occurs in oxytocin (OT)-secreting and vasopressin (AVP)-secreting neurons of the adult hypothalamo-neurohypophysial system (HNS) whose axons undergo morphological changes in response to stimulation. Immunocytochemistry and immunoblot analysis showed that during basal conditions of HNS secretion, there are higher levels of this glycosylphosphatidyl inositol-anchored protein in the neurohypophysis, where their axons terminate, than in the hypothalamic nuclei containing their somata. Physiological stimulation (lactation, osmotic challenge) reversed this pattern and resulted in upregulation of F3 expression, paralleling that of OT and AVP under these conditions. In situ hybridization revealed that F3 expression in the hypothalamus is restricted to its magnocellular neurons and demonstrated a more than threefold increase in F3 mRNA levels in response to stimulation. Confocal and electron microscopy localized F3 in secretory granules in all neuronal compartments, a localization confirmed by detection of F3 immunoreactivity in granule-enriched fractions obtained by sucrose density gradient fractionation of rat neurohypophyses. F3 was not visible on any cell surface in the magnocellular nuclei. In contrast, in the neurohypophysis, it was present not only in secretory granules but also on the surface of axon terminals and glia and in extracellular spaces.

Taken together, our observations reveal that the cell adhesion glycoprotein F3 is colocalized with neurohypophysial peptides in secretory granules. It follows, therefore, the regulated pathway of secretion in HNS neurons to be released by exocytosis at their axon terminals in the neurohypophysis, where it may intervene in activity-dependent structural axonal plasticity.

Key words: hypothalamo-neurohypophysial system; cell adhesion molecules; plasticity; lactation; dehydration; in situ hybridization; immunocytochemistry; immunoblot analysis

---

Copyright © 1998 Society for Neuroscience  0270-6474/98/18145333-11$05.00/0

This article has been cited by other articles:

				M. Landry, E. Vila-Porcile, and A. Calas Immunogold Detection of Co-localized Neuropeptides: Methodological Aspects J. Histochem. Cytochem., May 1, 2004; 52(5): 617 - 628. [Abstract] [Full Text] [PDF]

				D. T. Theodosis, R. Bonhomme, S. Vitiello, G. Rougon, and D. A. Poulain Cell Surface Expression of Polysialic Acid on NCAM Is a Prerequisite for Activity-Dependent Morphological Neuronal and Glial Plasticity J. Neurosci., December 1, 1999; 19(23): 10228 - 10236. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help