 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, July 15, 1998, 18(14):5517-5528
Glutamate Receptor Targeting to Synaptic Populations on Purkinje Cells Is Developmentally Regulated
Hui-Min Zhao, Robert J. Wenthold, and Ronald S. Petralia National Institute on Deafness and Other Communication Disorders/National Institutes of Health, Bethesda, Maryland 20892
Selective targeting of neurotransmitter receptors to specific synapse populations occurs in adult neurons, but little is known about the development of these receptor distribution patterns. In this study, we demonstrate that a specific developmental switch occurs in the targeting of a receptor to an identified synapse population. Localization of delta and AMPA glutamate receptors at parallel and climbing fiber synapses on the developing Purkinje cells was studied using postembedding immunogold. Delta receptors were found to be abundant on postsynaptic membranes at parallel fiber synapses from postnatal day 10 (P10) to adult. In contrast, delta receptors were found to be high at climbing fiber synapses only at P10 and P14. Thus, a major finding of this paper is that high levels of delta receptors are transiently expressed in climbing fiber synapses in the second postnatal week. Labeling of synapses with anti-delta receptor antibody at P10 was limited to the postsynaptic membrane of excitatory synapses and was absent from GABAergic synapses. Unlike delta receptor immunolabeling, AMPA receptor immunolabeling (GluR2/3 and GluR2 antibodies) was high in the postsynaptic membranes of synapses at early postnatal ages (P2 and P5) and was higher in climbing fiber synapses than in parallel fiber synapses from P10 to adult. The present study shows that synapse-specific targeting of glutamate receptors in Purkinje cells is developmentally regulated, with the postsynaptic receptor composition established during synapse maturation. This composition is not dependent on the nature of the initial establishment of synaptic connections.
Key words: AMPA; cerebellum; climbing fiber; delta; parallel fiber; synaptogenesis
Copyright © 1998 Society for Neuroscience 0270-6474/98/18145517-12$05.00/0
This article has been cited by other articles:
P. Naur, K. B. Hansen, A. S. Kristensen, S. M. Dravid, D. S. Pickering, L. Olsen, B. Vestergaard, J. Egebjerg, M. Gajhede, S. F. Traynelis, et al.
Ionotropic glutamate-like receptor {delta}2 binds D-serine and glycine
PNAS, August 28, 2007; 104(35): 14116 - 14121.
[Abstract] [Full Text] [PDF]
M. Masugi-Tokita, E. Tarusawa, M. Watanabe, E. Molnar, K. Fujimoto, and R. Shigemoto
Number and Density of AMPA Receptors in Individual Synapses in the Rat Cerebellum as Revealed by SDS-Digested Freeze-Fracture Replica Labeling
J. Neurosci., February 21, 2007; 27(8): 2135 - 2144.
[Abstract] [Full Text] [PDF]
K. Matsui, C. E. Jahr, and M. E. Rubio
High-Concentration Rapid Transients of Glutamate Mediate Neural-Glial Communication via Ectopic Release
J. Neurosci., August 17, 2005; 25(33): 7538 - 7547.
[Abstract] [Full Text] [PDF]
P. Lachamp, B. Balland, F. Tell, A. Baude, C. Strube, M. Crest, and J.-P. Kessler
Early expression of AMPA receptors and lack of NMDA receptors in developing rat climbing fibre synapses
J. Physiol., May 1, 2005; 564(3): 751 - 763.
[Abstract] [Full Text] [PDF]
T. Takeuchi, T. Miyazaki, M. Watanabe, H. Mori, K. Sakimura, and M. Mishina
Control of Synaptic Connection by Glutamate Receptor {delta}2 in the Adult Cerebellum
J. Neurosci., February 23, 2005; 25(8): 2146 - 2156.
[Abstract] [Full Text] [PDF]
N. C. Dembrow, J. Jing, V. Brezina, and K. R. Weiss
A Specific Synaptic Pathway Activates a Conditional Plateau Potential Underlying Protraction Phase in the Aplysia Feeding Central Pattern Generator
J. Neurosci., June 2, 2004; 24(22): 5230 - 5238.
[Abstract] [Full Text] [PDF]
Y. Kitao, K. Hashimoto, T. Matsuyama, H. Iso, T. Tamatani, O. Hori, D. M. Stern, M. Kano, K. Ozawa, and S. Ogawa
ORP150/HSP12A Regulates Purkinje Cell Survival: A Role for Endoplasmic Reticulum Stress in Cerebellar Development
J. Neurosci., February 11, 2004; 24(6): 1486 - 1496.
[Abstract] [Full Text] [PDF]
R. Cesa, L. Morando, and P. Strata
Glutamate Receptor delta 2 Subunit in Activity-Dependent Heterologous Synaptic Competition
J. Neurosci., March 15, 2003; 23(6): 2363 - 2370.
[Abstract] [Full Text] [PDF]
R. Ichikawa, T. Miyazaki, M. Kano, T. Hashikawa, H. Tatsumi, K. Sakimura, M. Mishina, Y. Inoue, and M. Watanabe
Distal Extension of Climbing Fiber Territory and Multiple Innervation Caused by Aberrant Wiring to Adjacent Spiny Branchlets in Cerebellar Purkinje Cells Lacking Glutamate Receptor delta 2
J. Neurosci., October 1, 2002; 22(19): 8487 - 8503.
[Abstract] [Full Text] [PDF]
D. A. Clayton, M. H. Mesches, E. Alvarez, P. C. Bickford, and M. D. Browning
A Hippocampal NR2B Deficit Can Mimic Age-Related Changes in Long-Term Potentiation and Spatial Learning in the Fischer 344 Rat
J. Neurosci., May 1, 2002; 22(9): 3628 - 3637.
[Abstract] [Full Text] [PDF]
Y. Miyagi, T. Yamashita, M. Fukaya, T. Sonoda, T. Okuno, K. Yamada, M. Watanabe, Y. Nagashima, I. Aoki, K. Okuda, et al.
Delphilin: a Novel PDZ and Formin Homology Domain-Containing Protein that Synaptically Colocalizes and Interacts with Glutamate Receptor delta 2 Subunit
J. Neurosci., February 1, 2002; 22(3): 803 - 814.
[Abstract] [Full Text] [PDF]
K. Hashimoto, R. Ichikawa, H. Takechi, Y. Inoue, A. Aiba, K. Sakimura, M. Mishina, T. Hashikawa, A. Konnerth, M. Watanabe, et al.
Roles of Glutamate Receptor delta 2 Subunit (GluRdelta 2) and Metabotropic Glutamate Receptor Subtype 1 (mGluR1) in Climbing Fiber Synapse Elimination during Postnatal Cerebellar Development
J. Neurosci., December 15, 2001; 21(24): 9701 - 9712.
[Abstract] [Full Text] [PDF]
J. L. Perez, L. Khatri, C. Chang, S. Srivastava, P. Osten, and E. B. Ziff
PICK1 Targets Activated Protein Kinase C{alpha} to AMPA Receptor Clusters in Spines of Hippocampal Neurons and Reduces Surface Levels of the AMPA-Type Glutamate Receptor Subunit 2
J. Neurosci., August 1, 2001; 21(15): 5417 - 5428.
[Abstract] [Full Text] [PDF]
L. Morando, R. Cesa, R. Rasetti, R. Harvey, and P. Strata
Role of glutamate delta -2 receptors in activity-dependent competition between heterologous afferent fibers
PNAS, August 1, 2001; (2001) 171098398.
[Abstract] [Full Text] [PDF]
A. W. McGee, J. R. Topinka, K. Hashimoto, R. S. Petralia, S. Kakizawa, F. Kauer, A. Aguilera-Moreno, R. J. Wenthold, M. Kano, and D. S. Bredt
PSD-93 Knock-Out Mice Reveal That Neuronal MAGUKs Are Not Required for Development or Function of Parallel Fiber Synapses in Cerebellum
J. Neurosci., May 1, 2001; 21(9): 3085 - 3091.
[Abstract] [Full Text] [PDF]
M. E. Rubio and F. Soto
Distinct Localization of P2X Receptors at Excitatory Postsynaptic Specializations
J. Neurosci., January 15, 2001; 21(2): 641 - 653.
[Abstract] [Full Text] [PDF]
N. Sans, R. S. Petralia, Y.-X. Wang, J. Blahos II, J. W. Hell, and R. J. Wenthold
A Developmental Change in NMDA Receptor-Associated Proteins at Hippocampal Synapses
J. Neurosci., February 1, 2000; 20(3): 1260 - 1271.
[Abstract] [Full Text] [PDF]
K. W. Roche, J. C. Tu, R. S. Petralia, B. Xiao, R. J. Wenthold, and P. F. Worley
Homer 1b Regulates the Trafficking of Group I Metabotropic Glutamate Receptors
J. Biol. Chem., September 3, 1999; 274(36): 25953 - 25957.
[Abstract] [Full Text] [PDF]
K. W. Roche, C. D. Ly, R. S. Petralia, Y.-X. Wang, A. W. McGee, D. S. Bredt, and R. J. Wenthold
Postsynaptic Density-93 Interacts with the delta 2 Glutamate Receptor Subunit at Parallel Fiber Synapses
J. Neurosci., May 15, 1999; 19(10): 3926 - 3934.
[Abstract] [Full Text] [PDF]
L. Morando, R. Cesa, R. Rasetti, R. Harvey, and P. Strata
Role of glutamate delta -2 receptors in activity-dependent competition between heterologous afferent fibers
PNAS, August 14, 2001; 98(17): 9954 - 9959.
[Abstract] [Full Text] [PDF]
K. Kawa
Acute synaptic modulation by nicotinic agonists in developing cerebellar Purkinje cells of the rat
J. Physiol., January 1, 2002; 538(1): 87 - 102.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|