 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, August 1, 1998, 18(15):5586-5593
Cloning and Functional Expression of an Aplysia 5-HT Receptor Negatively Coupled to Adenylate Cyclase
Annie Angers1, Maksim V. Storozhuk2, Thomas Duchaîne1, Vincent F. Castellucci2, 3, and Luc DesGroseillers1, 3 1 Département de biochimie, 2 Département de physiologie, and 3 Centre de recherches en sciences neurologiques, Université de Montréal, Montréal, Québec, Canada H3C 3J7
Serotonin (5-HT) is involved in the control of various behaviors in Aplysia californica, including reproduction, feeding, locomotion, circadian rhythm, synaptic plasticity, and synaptic growth. The large variety of functions of 5-HT is mediated by different receptor subtypes that are coupled to different second-messenger systems. Here, we report the cloning of a cDNA coding for an Aplysia G-protein-coupled 5-HT receptor (5-HTap1). Its deduced amino acid sequence resembles those of the 5-HT1 receptor subfamily. When expressed in stable cell lines, 5-HTap1 exhibits high-affinity binding for the serotonergic radioligand [N-methyl-3H]lysergic acid diethylamide. This binding is competed by several 5-HT agonists and antagonists, and the pharmacological profile of inhibition has some similarities with those of 5-HT1 and 5-HT7 receptors. Application of 5-HT or its agonists 5-carboxamidotryptamine maleate and (±)-8-hydroxy-2-(di-n-propyl-amino) tetralin hydrobromide on cells transformed with 5-HTap1 produced a dose-dependent inhibition of forskolin-stimulated cAMP accumulation. 5-HTap1 is thus negatively coupled to adenylate cyclase. The production of antiserum against the 5-HTap1 receptor allowed us to examine its expression in animal tissues. The receptor protein is detected in every tissue examined, although it seems only weakly expressed in some samples. The receptor is also found in every ganglia of the nervous system, both in the sheath and in the neurons. 5-HTap1 mRNA is absent from the sheath, indicating that the protein observed there is probably located on the nerve terminals.
Key words: Aplysia; 5-HT; 5-HT1 receptor; G-protein-coupled receptor; adenylate cyclase; inhibition
Copyright © 1998 Society for Neuroscience 0270-6474/98/18155586-08$05.00/0
This article has been cited by other articles:
S. Mapara, S. Parries, C. Quarrington, K.-C. Ahn, W. J. Gallin, and J. I. Goldberg
Identification, molecular structure and expression of two cloned serotonin receptors from the pond snail, Helisoma trivolvis
J. Exp. Biol., March 15, 2008; 211(6): 900 - 910.
[Abstract] [Full Text] [PDF]
N. Spitzer, D. H. Edwards, and D. J. Baro
Conservation of structure, signaling and pharmacology between two serotonin receptor subtypes from decapod crustaceans, Panulirus interruptus and Procambarus clarkii
J. Exp. Biol., January 1, 2008; 211(1): 92 - 105.
[Abstract] [Full Text] [PDF]
B. Dumitriu, J. E. Cohen, Q. Wan, A. M. Negroiu, and T. W. Abrams
Serotonin Receptor Antagonists Discriminate Between PKA- and PKC-Mediated Plasticity in Aplysia Sensory Neurons
J Neurophysiol, April 1, 2006; 95(4): 2713 - 2720.
[Abstract] [Full Text] [PDF]
S. Marinesco, K. E. Kolkman, and T. J. Carew
Serotonergic Modulation in Aplysia. I. Distributed Serotonergic Network Persistently Activated by Sensitizing Stimuli
J Neurophysiol, October 1, 2004; 92(4): 2468 - 2486.
[Abstract] [Full Text] [PDF]
C. M. Sherff and T. J. Carew
Parallel somatic and synaptic processing in the induction of intermediate-term and long-term synaptic facilitation in Aplysia
PNAS, May 11, 2004; 101(19): 7463 - 7468.
[Abstract] [Full Text] [PDF]
A. Meneses
A Pharmacological Analysis of an Associative Learning Task: 5-HT1 to 5-HT7 Receptor Subtypes Function on a Pavlovian/Instrumental Autoshaped Memory
Learn. Mem., September 1, 2003; 10(5): 363 - 372.
[Abstract] [Full Text] [PDF]
D. Barbas, L. DesGroseillers, V. F. Castellucci, T. J. Carew, and S. Marinesco
Multiple Serotonergic Mechanisms Contributing to Sensitization in Aplysia: Evidence of Diverse Serotonin Receptor Subtypes
Learn. Mem., September 1, 2003; 10(5): 373 - 386.
[Abstract] [Full Text] [PDF]
H. Zhang, M. Wainwright, J. H. Byrne, and L. J. Cleary
Quantitation of Contacts Among Sensory, Motor, and Serotonergic Neurons in the Pedal Ganglion of Aplysia
Learn. Mem., September 1, 2003; 10(5): 387 - 393.
[Abstract] [Full Text] [PDF]
J. E. Cohen, C. U. Onyike, V. L. McElroy, A. H. Lin, and T. W. Abrams
Pharmacological Characterization of an Adenylyl Cyclase-Coupled 5-HT Receptor in Aplysia: Comparison With Mammalian 5-HT Receptors
J Neurophysiol, March 1, 2003; 89(3): 1440 - 1455.
[Abstract] [Full Text] [PDF]
L. E. Fox and P. E. Lloyd
Mechanisms Involved in Persistent Facilitation of Neuromuscular Synapses in Aplysia
J Neurophysiol, April 1, 2002; 87(4): 2018 - 2030.
[Abstract] [Full Text] [PDF]
S. Marinesco and T. J. Carew
Serotonin Release Evoked by Tail Nerve Stimulation in the CNS of Aplysia: Characterization and Relationship to Heterosynaptic Plasticity
J. Neurosci., March 15, 2002; 22(6): 2299 - 2312.
[Abstract] [Full Text] [PDF]
S. Clemens and P. S. Katz
Identified Serotonergic Neurons in the Tritonia Swim CPG Activate Both Ionotropic and Metabotropic Receptors
J Neurophysiol, January 1, 2001; 85(1): 476 - 479.
[Abstract] [Full Text] [PDF]
X. Liao, C. G. Brou, and E. T. Walters
Limited Contributions of Serotonin to Long-Term Hyperexcitability of Aplysia Sensory Neurons
J Neurophysiol, December 1, 1999; 82(6): 3223 - 3235.
[Abstract] [Full Text] [PDF]
J. Levenson, J. H. Byrne, and A. Eskin
Levels of Serotonin in the Hemolymph of Aplysia Are Modulated by Light/Dark Cycles and Sensitization Training
J. Neurosci., September 15, 1999; 19(18): 8094 - 8103.
[Abstract] [Full Text] [PDF]
J. P. Zappulla, L. Wickham, W. Bawab, X.-F. Yang, M. V. Storozhuk, V. F. Castellucci, and L. DesGroseillers
Cloning and Characterization of Aplysia Neutral Endopeptidase, a Metallo-Endopeptidase Involved in the Extracellular Metabolism of Neuropeptides in Aplysia californica
J. Neurosci., June 1, 1999; 19(11): 4280 - 4292.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|