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The Journal of Neuroscience, August 1, 1998, 18(15):5928-5937
Differential Distribution of  2A and
2C Adrenergic Receptor Immunoreactivity in the Rat
Spinal Cord
Laura S.
Stone1, 2, 3,
Christian
Broberger4,
Lucy
Vulchanova1, 3,
George L.
Wilcox1, 2,
Tomas
Hökfelt4,
Maureen S.
Riedl3, and
Robert
Elde1, 2, 3
1 Graduate Program in Neuroscience and Departments of
2 Pharmacology, and 3 Cell Biology and
Neuroanatomy, University of Minnesota, Minneapolis, Minnesota 55455, and 4 Department of Neuroscience, Karolinska
Institutet, 17177 Stockholm, Sweden
 2-Adrenergic receptors (2-ARs)
mediate a number of physiological phenomena, including spinal
analgesia. We have developed subtype-selective antisera against the C
termini of the 2A-AR and 2C-AR to
investigate the relative distribution and cellular source or sources of
these receptor subtypes in the rat spinal cord. Immunoreactivity
(IR) for both receptor subtypes was observed in the superficial
layers of the dorsal horn of the spinal cord. Our results suggest that
the primary localization of the 2A-AR in the rat spinal
cord is on the terminals of capsaicin-sensitive, substance P
(SP)-containing primary afferent fibers. In contrast, the majority of
2C-AR-IR was not of primary afferent origin, not
strongly colocalized with SP-IR, and not sensitive to neonatal capsaicin treatment. Spinal 2C-AR-IR does not appear to
colocalize with the neurokinin-1 receptor, nor is it localized
on astrocytes, as evidenced by a lack of costaining with the glial
marker GFAP. However, some colocalization was observed between
2C-AR-IR and enkephalin-IR, suggesting that the
2C-AR may be expressed by a subset of spinal
interneurons. Interestingly, neither subtype was detected on descending
noradrenergic terminals. These results indicate that the
2-AR subtypes investigated are likely expressed by
different subpopulations of neurons and may therefore subserve different physiological functions in the spinal cord, with the 2A-AR being more likely to play a role in the modulation
of nociceptive information.
Key words:
2-adrenergic receptor; spinal cord; immunohistochemistry; RG10; RG20; capsaicin; dorsal rhizotomy; confocal; rat; substance P; noradrenaline; analgesia; 2A; 2C
Copyright © 1998 Society for Neuroscience 0270-6474/98/18155928-10$05.00/0
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