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The Journal of Neuroscience, August 15, 1998, 18(16):6331-6339

Innervation and Target Tissue Interactions Induce Rab-GDP Dissociation Inhibitor (GDI) Expression during Peripheral Synapse Formation in Developing Chick Ciliary Ganglion Neurons In Situ

Ognian C. Ikonomov¹, Marianne C. Kulesa¹, Assia C. Shisheva², and Michele H. Jacob¹

¹ Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545, and ² Program in Molecular Medicine, Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, Worcester, Massachusetts 01605

Regulated exocytosis of neurotransmitter from synaptic vesicles involves the function of a small GTP-binding protein, Rab3A. Rab-GDP dissociation inhibitor (GDI) is an important modulator of Rab function and subcellular distribution. We have characterized the respective roles of innervation and target tissue interactions in regulating GDI expression during synapse formation in chick ciliary ganglion (CG) neurons developing in situ. Here we report the first full-length chick GDI cDNA sequence. It is highly homologous to mammalian GDI isoforms and includes all of the sequence-conserved regions critical for Rab3A binding. This chick GDI mRNA is predominantly expressed in neurons as judged by Northern blot analysis of tissue distribution and by in situ hybridization of CG sections. Developmental increases in CG GDI mRNA levels occur in two phases as determined by reverse transcription (RT)-PCR and by Northern analysis of both normal-developing and input- or target tissue-deprived ganglia. The initial phase appears to be independent of cell-cell interactions. In contrast, the second, larger increase is induced by both presynaptic inputs and postganglionic target tissues but does not occur until target tissue innervation. Synaptic interaction with the target seems necessary for the regulatory response to both inputs and target tissues. GDI protein levels show similar changes. The developmentally delayed ability of inputs and targets to influence GDI levels differs from the regulation of neurotransmitter receptor expression in CG neurons. These results suggest that distinct extrinsic regulatory signals influence the expression of synapse-related components at the presynaptic axon terminal versus postsynaptic membrane in an individual neuron.

Key words: chick Rab-GDP dissociation inhibitor (GDI); embryonic chick ciliary ganglion; neuronal synapse formation; neuron-specific gene expression; induction; regulation; innervation; target tissue interactions; presynaptic terminal differentiation; synaptic vesicle exocytosis

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Copyright © 1998 Society for Neuroscience  0270-6474/98/18166331-09$05.00/0

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