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The Journal of Neuroscience, August 15, 1998, 18(16):6549-6557

Neurofilament Proteins in Y-Cells of the Cat Lateral Geniculate Nucleus: Normal Expression and Alteration with Visual Deprivation

Martha E. Bickford¹, William Guido², and Dwayne W. Godwin³

¹ Department of Anatomical Sciences and Neurobiology, University of Louisville, School of Medicine, Louisville, Kentucky 40292, ² Department of Cell Biology and Anatomy, Louisiana State University Medical Center, New Orleans, Louisiana 70112, and ³ Department of Neurobiology and Anatomy, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157-1010

We examined neurofilament staining in the normal and visually deprived lateral geniculate nucleus (LGN), using the SMI-32 antibody. This antibody preferentially stains LGN cells that display the morphological characteristics of Y-cells. The soma sizes of SMI-32-stained cells were consistent with those of the overall population of Y-cells, and the Golgi-like staining of their dendrites revealed a radial distribution that often crossed laminar boundaries. Labeled cells were distributed within the A laminae (primarily near laminar borders), the magnocellular portion of the C laminae, and the medial intralaminar nucleus, but they were absent in the parvocellular C laminae. Electron microscopic examination of SMI-32-stained tissue revealed that staining was confined to somata, dendrites, and large myelinated axons. Retinal synapses on SMI-32-labeled dendrites were primarily simple axodendritic contacts; few triadic arrangements were observed. In the LGN of cats reared with monocular lid suture, SMI-32 staining was decreased significantly in the A laminae that received input from the deprived eye. Dephosphorylation of the tissue did not alter the cellular SMI-32 staining patterns. Analysis of staining patterns in the C laminae and monocular zone of the A laminae suggests that changes in the cytoskeleton after lid suture reflect cell class and not binocular competition. Taken together, the results from normal and lid-sutured animals suggest that the cat LGN offers a unique model system in which the cytoskeleton of one class of cells can be manipulated by altering neuronal activity.

Key words: SMI-32; electron microscopy; monocular deprivation; immunocytochemistry; thalamus; cytoskeleton

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Copyright © 1998 Society for Neuroscience  0270-6474/98/18166549-09$05.00/0

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