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The Journal of Neuroscience, September 15, 1998, 18(18):7244-7255
The Nitric Oxide-cGMP Pathway May Mediate Communication between
Sensory Afferents and Projection Neurons in the Antennal Lobe of
Manduca Sexta
Alan
Nighorn1,
Nicholas
J.
Gibson1,
David M.
Rivers1,
John G.
Hildebrand1, and
David B.
Morton2
1 Arizona Research Laboratories, Division of
Neurobiology, University of Arizona, Tucson, Arizona 85721, and
2 Department of Biological Structure and Function, School
of Dentistry, Oregon Health Sciences University, Portland, Oregon 97201
The nitric oxide (NO)-cGMP signaling system is thought to play
important roles in the function of the olfactory system in both
vertebrates and invertebrates. One way of studying the role of NO in
the nervous system is to study the distribution and properties of NO
synthase (NOS), as well as the soluble guanylyl cyclases (sGCs), which
are the best characterized targets of NO. We study NOS and sGC in the
relatively simple and well characterized insect olfactory system of the
hawkmoth, Manduca sexta. We have cloned Manduca
sexta nitric oxide synthase (MsNOS) and two sGCs (MsGC 1 and
MsGC 1), characterized their basic biochemical properties, and
studied their expression in the olfactory system. The sequences of the
Manduca genes are highly similar to their mammalian
homologs and show similar biochemical properties when expressed in
COS-7 cells. In particular, we find that MsGC functions as an
obligate heterodimer that is stimulated significantly by NO. We also
find that MsNOS has a Ca2+-sensitive NO-producing
activity similar to that of mammalian neuronal NOS. Northern and
in situ hybridization analyses show that MsNOS and the
MsGCs are expressed in a complementary pattern, with MsNOS expressed at
high levels in the antennae and the MsGCs expressed at high levels in a
subset of antennal lobe neurons. The expression patterns of these genes
suggest that the NO-sGC signaling system may play a role in mediating
communication between olfactory receptor neurons and projection neurons
in the glomeruli of the antennal lobe.
Key words:
nitric oxide synthase; soluble guanylyl cyclase; Manduca sexta; olfaction; cGMP; NO
Copyright © 1998 Society for Neuroscience 0270-6474/98/18187244-12$05.00/0
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