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The Journal of Neuroscience, September 15, 1998, 18(18):7315-7327
Embryonic Expression of the Myelin Basic Protein Gene:
Identification of a Promoter Region That Targets Transgene Expression
to Pioneer Neurons
Charles F.
Landry1,
Thomas M.
Pribyl1,
Julie A.
Ellison1,
M. Irene
Givogri1,
Kathy
Kampf1,
Celia W.
Campagnoni1, and
Anthony T.
Campagnoni1, 2
1 Developmental Biology Group, Neuropsychiatric
Institute, and 2 Brain Research Institute, University of
California at Los Angeles, School of Medicine, Los Angeles, California
90024
The myelin basic protein (MBP) gene produces two families of
structurally related proteins from three different promoters the golli
products, generated from the most upstream promoter, and the MBPs,
produced from the two downstream promoters. In this report we describe
the expression of golli proteins within some of the earliest neuronal
populations of the brain, including Cajal-Retzius cells and preplate
neurons of the forebrain, representing a new marker for these cells. To
identify elements responsible for neuronal expression of the golli
products, we generated transgenic animals from constructs containing
different portions of the upstream promoter. A construct containing 1.1 kb immediately upstream of the golli transcription start site targeted
expression of  -galactosidase to preplate neurons and a subset of
Cajal-Retzius cells in transgenic micethe first reported genetic
element to target expression to these pioneer cortical populations.
Although expression in Cajal-Retzius cells declined with embryonic
development, preplate cells continued to express the transgene after
arriving at their final destination in the subplate. Interestingly,
expression persisted in subplate neurons found within a distinct layer
between the white matter and cortical layer VI well into postnatal
life. Birth dating studies with bromodeoxyuridine indicated that these
neurons were born between E10.5 and E12.5. Thus, the transgene marked
subplate neurons from their birth, providing a fate marker for these
cells. This work suggests a role for the MBP gene in the early
developing brain long before myelination and especially in the pioneer
cortical neurons important in the formation of the cortical layers.
Key words:
subplate neurons; Cajal-Retzius neurons; pioneer
neurons; golli-mbp gene; promoter elements
Copyright © 1998 Society for Neuroscience 0270-6474/98/18187315-13$05.00/0
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