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The Journal of Neuroscience, September 15, 1998, 18(18):7361-7371
Cyclic AMP Elevation Is Sufficient to Promote the Survival of Spinal Motor Neurons In Vitro
Martin G. Hanson Jr1, Shiliang Shen1, Anthony P. Wiemelt2, F. Arthur McMorris2, and Ben A. Barres1 1 Stanford University School of Medicine, Department of Neurobiology, Stanford, California 94305-5125, and 2 Wistar Institute, Philadelphia, Pennsylvania 19104
The short-term survival of highly purified embryonic spinal motor neurons (SMNs) in culture can be promoted by many peptide trophic factors, including brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), fibroblast growth factor (FGF), glial-derived neurotrophic factor (GDNF), and hepatocyte growth factor (HGF). We have asked whether these peptides are sufficient to promote the long-term survival of purified E15 SMNs. Contrary to previous reports, we find that when SMNs are cultured in serum-free medium containing a single peptide trophic factor only approximately one-third of the cells survive for 3 d in culture. When multiple factors are combined, additive effects on survival are observed transiently, but by 7 d of culture the majority of SMNs has died. Surprisingly, when cAMP levels are elevated, the majority of SMNs extend processes and survive for 1 week in culture in the absence of peptide trophic factors, even in low-density cultures. A combination of five peptide trophic factors, together with cAMP elevation, promotes the long-term survival of most of the SMNs in serum-free culture for 3 weeks. These findings provide useful culture conditions for studying the properties of SMNs and have implications for the treatment of motor neuron diseases.
Key words: rat; trophic factors; forskolin; IBMX; injury; regeneration; bone morphogenetic proteins
Copyright © 1998 Society for Neuroscience 0270-6474/98/18187361-11$05.00/0
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