 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, October 1, 1998, 18(19):7709-7716
Glial Contribution to Glutamate Uptake at Schaffer Collateral-Commissural Synapses in the Hippocampus
Dwight E. Bergles and Craig E. Jahr Vollum Institute, Oregon Health Sciences University, Portland, Oregon 97201
Astrocytes in the hippocampus express high-affinity glutamate transporters that are important for lowering the concentration of extracellular glutamate after release at excitatory synapses. These transporters exhibit a permeability to chaotropic anions that is associated with transport, allowing their activity to be monitored in cell-fee patches when highly permeant anions are present. Astrocyte glutamate transporters are highly temperature sensitive, because L-glutamate-activated, anion-potentiated transporter currents in outside-out patches from these cells exhibited larger amplitudes and faster kinetics at 36°C than at 24°C. The cycling rate of these transporters was estimated by using paired applications of either L-glutamate or D-aspartate to measure the time necessary for the peak of the transporter current to recover from the steady-state level. Transporter currents in patches recovered with a time constant of 11.6 msec at 36°C, suggesting that either the turnover rate of native transporters is much faster than previously reported for expressed EAAT2 transporters or the efficiency of these transporters is very low. Synaptically activated transporter currents persisted in astrocytes at physiological temperatures, although no evidence of these currents was found in CA1 pyramidal neurons in response to afferent stimulation. L-glutamate-gated transporter currents were also not detected in outside-out patches from pyramidal neurons. These results are consistent with the hypothesis that astrocyte transporters are responsible for taking up the majority of glutamate released at Schaffer collateral-commissural synapses in the hippocampus.
Key words: glutamate transporter; astrocyte; GLT-1; GLAST; EAAC1; hippocampus; CA1
Copyright © 1998 Society for Neuroscience 0270-6474/98/18197709-08$05.00/0
This article has been cited by other articles:
M. Dallas, H. E. Boycott, L. Atkinson, A. Miller, J. P. Boyle, H. A. Pearson, and C. Peers
Hypoxia Suppresses Glutamate Transport in Astrocytes
J. Neurosci., April 11, 2007; 27(15): 3946 - 3955.
[Abstract] [Full Text] [PDF]
M. S Parihar and G. J. Brewer
Mitoenergetic failure in Alzheimer disease
Am J Physiol Cell Physiol, January 1, 2007; 292(1): C8 - C23.
[Abstract] [Full Text] [PDF]
E. Glowatzki, N. Cheng, H. Hiel, E. Yi, K. Tanaka, G. C. R. Ellis-Davies, J. D. Rothstein, and D. E. Bergles
The glutamate-aspartate transporter GLAST mediates glutamate uptake at inner hair cell afferent synapses in the mammalian cochlea.
J. Neurosci., July 19, 2006; 26(29): 7659 - 7664.
[Abstract] [Full Text] [PDF]
J. M. Christie and G. L. Westbrook
Lateral excitation within the olfactory bulb.
J. Neurosci., February 22, 2006; 26(8): 2269 - 2277.
[Abstract] [Full Text] [PDF]
J. M. Christie and C. E. Jahr
Multivesicular Release at Schaffer Collateral-CA1 Hippocampal Synapses
J. Neurosci., January 4, 2006; 26(1): 210 - 216.
[Abstract] [Full Text] [PDF]
C. Mim, P. Balani, T. Rauen, and C. Grewer
The Glutamate Transporter Subtypes EAAT4 and EAATs 1-3 Transport Glutamate with Dramatically Different Kinetics and Voltage Dependence but Share a Common Uptake Mechanism
J. Gen. Physiol., November 28, 2005; 126(6): 571 - 589.
[Abstract] [Full Text] [PDF]
J. S. Diamond
Deriving the Glutamate Clearance Time Course from Transporter Currents in CA1 Hippocampal Astrocytes: Transmitter Uptake Gets Faster during Development
J. Neurosci., March 16, 2005; 25(11): 2906 - 2916.
[Abstract] [Full Text] [PDF]
A. Scimemi, A. Fine, D. M. Kullmann, and D. A. Rusakov
NR2B-Containing Receptors Mediate Cross Talk among Hippocampal Synapses
J. Neurosci., May 19, 2004; 24(20): 4767 - 4777.
[Abstract] [Full Text] [PDF]
Y. H. Huang, S. R. Sinha, K. Tanaka, J. D. Rothstein, and D. E. Bergles
Astrocyte Glutamate Transporters Regulate Metabotropic Glutamate Receptor-Mediated Excitation of Hippocampal Interneurons
J. Neurosci., May 12, 2004; 24(19): 4551 - 4559.
[Abstract] [Full Text] [PDF]
G. Brasnjo and T. S. Otis
Isolation of glutamate transport-coupled charge flux and estimation of glutamate uptake at the climbing fiber-Purkinje cell synapse
PNAS, April 20, 2004; 101(16): 6273 - 6278.
[Abstract] [Full Text] [PDF]
W. Chen, V. Mahadomrongkul, U. V. Berger, M. Bassan, T. DeSilva, K. Tanaka, N. Irwin, C. Aoki, and P. A. Rosenberg
The Glutamate Transporter GLT1a Is Expressed in Excitatory Axon Terminals of Mature Hippocampal Neurons
J. Neurosci., February 4, 2004; 24(5): 1136 - 1148.
[Abstract] [Full Text] [PDF]
K. J. Todd, C. A. B. Slatter, and D. W. Ali
Activation of Ionotropic Glutamate Receptors on Peripheral Axons of Primary Motoneurons Mediates Transmitter Release at the Zebrafish NMJ
J Neurophysiol, February 1, 2004; 91(2): 828 - 840.
[Abstract] [Full Text] [PDF]
S. Lei, K. A. Pelkey, L. Topolnik, P. Congar, J.-C. Lacaille, and C. J. McBain
Depolarization-Induced Long-Term Depression at Hippocampal Mossy Fiber-CA3 Pyramidal Neuron Synapses
J. Neurosci., October 29, 2003; 23(30): 9786 - 9795.
[Abstract] [Full Text] [PDF]
K. Matthias, F. Kirchhoff, G. Seifert, K. Huttmann, M. Matyash, H. Kettenmann, and C. Steinhauser
Segregated Expression of AMPA-Type Glutamate Receptors and Glutamate Transporters Defines Distinct Astrocyte Populations in the Mouse Hippocampus
J. Neurosci., March 1, 2003; 23(5): 1750 - 1758.
[Abstract] [Full Text] [PDF]
A. Bordey and H. Sontheimer
Modulation of Glutamatergic Transmission by Bergmann Glial Cells in Rat Cerebellum In Situ
J Neurophysiol, February 1, 2003; 89(2): 979 - 988.
[Abstract] [Full Text] [PDF]
G. A. Kinney and W. J. Spain
Synaptically Evoked GABA Transporter Currents in Neocortical Glia
J Neurophysiol, December 1, 2002; 88(6): 2899 - 2908.
[Abstract] [Full Text] [PDF]
D. E. Bergles, A. V. Tzingounis, and C. E. Jahr
Comparison of Coupled and Uncoupled Currents during Glutamate Uptake by GLT-1 Transporters
J. Neurosci., December 1, 2002; 22(23): 10153 - 10162.
[Abstract] [Full Text] [PDF]
R. D. Fields and B. Stevens-Graham
NEUROSCIENCE: New Insights into Neuron-Glia Communication
Science, October 18, 2002; 298(5593): 556 - 562.
[Abstract] [Full Text] [PDF]
Y. Ikegaya, S. Matsuura, S. Ueno, A. Baba, M. K. Yamada, N. Nishiyama, and N. Matsuki
beta -Amyloid Enhances Glial Glutamate Uptake Activity and Attenuates Synaptic Efficacy
J. Biol. Chem., August 23, 2002; 277(35): 32180 - 32186.
[Abstract] [Full Text] [PDF]
K. P. Lehre and D. A. Rusakov
Asymmetry of Glia near Central Synapses Favors Presynaptically Directed Glutamate Escape
Biophys. J., July 1, 2002; 83(1): 125 - 134.
[Abstract] [Full Text] [PDF]
D. Robbe, G. Alonso, S. Chaumont, J. Bockaert, and O. J. Manzoni
Role of P/Q-Ca2+ Channels in Metabotropic Glutamate Receptor 2/3-Dependent Presynaptic Long-Term Depression at Nucleus Accumbens Synapses
J. Neurosci., June 1, 2002; 22(11): 4346 - 4356.
[Abstract] [Full Text] [PDF]
A. Araque, E. D. Martin, G. Perea, J. I. Arellano, and W. Buno
Synaptically Released Acetylcholine Evokes Ca2+ Elevations in Astrocytes in Hippocampal Slices
J. Neurosci., April 1, 2002; 22(7): 2443 - 2450.
[Abstract] [Full Text] [PDF]
J. S. Diamond
Neuronal Glutamate Transporters Limit Activation of NMDA Receptors by Neurotransmitter Spillover on CA1 Pyramidal Cells
J. Neurosci., November 1, 2001; 21(21): 8328 - 8338.
[Abstract] [Full Text] [PDF]
A. M. Sebastiao, A. de Mendonca, T. Moreira, and J. A. Ribeiro
Activation of Synaptic NMDA Receptors by Action Potential-Dependent Release of Transmitter during Hypoxia Impairs Recovery of Synaptic Transmission on Reoxygenation
J. Neurosci., November 1, 2001; 21(21): 8564 - 8571.
[Abstract] [Full Text] [PDF]
M. Zhou and H. K. Kimelberg
Freshly Isolated Hippocampal CA1 Astrocytes Comprise Two Populations Differing in Glutamate Transporter and AMPA Receptor Expression
J. Neurosci., October 15, 2001; 21(20): 7901 - 7908.
[Abstract] [Full Text] [PDF]
B. Barbour
An Evaluation of Synapse Independence
J. Neurosci., October 15, 2001; 21(20): 7969 - 7984.
[Abstract] [Full Text] [PDF]
K. L. Behar and D. L. Rothman
In Vivo Nuclear Magnetic Resonance Studies of Glutamate-{gamma}-Aminobutyric Acid-Glutamine Cycling in Rodent and Human Cortex: the Central Role of Glutamine
J. Nutr., September 1, 2001; 131(9): 2498S - 2504.
[Abstract] [Full Text] [PDF]
S. H. R. Oliet, R. Piet, and D. A. Poulain
Control of Glutamate Clearance and Synaptic Efficacy by Glial Coverage of Neurons
Science, May 4, 2001; 292(5518): 923 - 926.
[Abstract] [Full Text] [PDF]
N. Chen, J. Ren, L. A. Raymond, and T. H. Murphy
Changes in Agonist Concentration Dependence That Are a Function of Duration of Exposure Suggest N-Methyl-D-aspartate Receptor Nonsaturation during Synaptic Stimulation
Mol. Pharmacol., February 1, 2001; 59(2): 212 - 219.
[Abstract] [Full Text]
E. M. Ullian, S. K. Sapperstein, K. S. Christopherson, and B. A. Barres
Control of Synapse Number by Glia
Science, January 26, 2001; 291(5504): 657 - 661.
[Abstract] [Full Text]
L. S. Overstreet, M. V. Jones, and G. L. Westbrook
Slow Desensitization Regulates the Availability of Synaptic GABAA Receptors
J. Neurosci., November 1, 2000; 20(21): 7914 - 7921.
[Abstract] [Full Text] [PDF]
F. Steigerwald, T. W. Schulz, L. T. Schenker, M. B. Kennedy, P. H. Seeburg, and G. Kohr
C-Terminal Truncation of NR2A Subunits Impairs Synaptic But Not Extrasynaptic Localization of NMDA Receptors
J. Neurosci., June 15, 2000; 20(12): 4573 - 4581.
[Abstract] [Full Text] [PDF]
J. S. Diamond and C. E. Jahr
Synaptically Released Glutamate Does Not Overwhelm Transporters on Hippocampal Astrocytes During High-Frequency Stimulation
J Neurophysiol, May 1, 2000; 83(5): 2835 - 2843.
[Abstract] [Full Text] [PDF]
T. S. Otis and M. P. Kavanaugh
Isolation of Current Components and Partial Reaction Cycles in the Glial Glutamate Transporter EAAT2
J. Neurosci., April 15, 2000; 20(8): 2749 - 2757.
[Abstract] [Full Text] [PDF]
Z.-C. Ye, J. D. Rothstein, and H. Sontheimer
Compromised Glutamate Transport in Human Glioma Cells: Reduction-Mislocalization of Sodium-Dependent Glutamate Transporters and Enhanced Activity of Cystine-Glutamate Exchange
J. Neurosci., December 15, 1999; 19(24): 10767 - 10777.
[Abstract] [Full Text] [PDF]
F. Sakai and K. Amaha
The Effects of Hypothermia on a Cloned Human Brain Glutamate Transporter (hGLT-1) Expressed in Chinese Hamster Ovary Cells: -[3H]L-Glutamate Uptake Study
Anesth. Analg., December 1, 1999; 89(6): 1546 - 1546.
[Abstract] [Full Text] [PDF]
S. Mennerick, W. Shen, W. Xu, A. Benz, K. Tanaka, K. Shimamoto, K. E. Isenberg, J. E. Krause, and C. F. Zorumski
Substrate Turnover by Transporters Curtails Synaptic Glutamate Transients
J. Neurosci., November 1, 1999; 19(21): 9242 - 9251.
[Abstract] [Full Text] [PDF]
R. Ventura and K. M. Harris
Three-Dimensional Relationships between Hippocampal Synapses and Astrocytes
J. Neurosci., August 15, 1999; 19(16): 6897 - 6906.
[Abstract] [Full Text] [PDF]
L. Hertz, S. R. Robinson;, J. L. Griffin;, P. J. Magistretti, L. Pellerin, R. G. Shulman, and D. L. Rothman;
Energy for Neurotransmission
Science, July 30, 1999; 285(5428): 639a - 639.
[Full Text]
J. A. Dzubay and C. E. Jahr
The Concentration of Synaptically Released Glutamate Outside of the Climbing Fiber-Purkinje Cell Synaptic Cleft
J. Neurosci., July 1, 1999; 19(13): 5265 - 5274.
[Abstract] [Full Text] [PDF]
M. H. Higgs and P. D. Lukasiewicz
Glutamate Uptake Limits Synaptic Excitation of Retinal Ganglion Cells
J. Neurosci., May 15, 1999; 19(10): 3691 - 3700.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|