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The Journal of Neuroscience, October 1, 1998, 18(19):7811-7821
Hair Cells and Supporting Cells Share a Common Progenitor in the Avian Inner Ear
Donna M. Fekete1, Shanthini Muthukumar2, and Domna Karagogeos3 1 Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, 2 Department of Biology, Boston College, Chestnut Hill, Massachusetts 02167, and 3 Department of Basic Sciences, University of Crete Medical School, Heraklion 711 10, Crete, and the Institute for Molecular Biology and Biotechnology, Heraklion, Crete, Greece
Sensory organs of the vertebrate inner ear contain two major cell types: hair cells (HCs) and supporting cells (SCs). To study the lineage relationships between these two populations, replication-defective retroviral vectors encoding marker genes were delivered to the otic vesicle of the chicken embryo. The resulting labeled clones were analyzed in the hearing organ of the chicken, called the basilar papilla (BP), after cellular differentiation. BPs were allowed to develop for 2 weeks after delivery of the retrovirus, were removed, and were processed histochemically as whole mounts to identify clones of cells. Clusters of labeled cells were evident in the sensory epithelium, the nonsensory epithelium, and in adjacent tissues. Labeled cell types included HCs, two morphologically distinct types of SCs, homogene cells, border cells, hyaline cells, ganglion cells, and connective tissue cells. Each clone was sectioned and cell-type identification was performed on sensory clones expressing retrovirally transduced
-galactosidase. Cell composition was determined for 41 sensory clones, most of which contained both HCs and SCs. Clones containing one HC and one SC were observed, suggesting that a common progenitor exists that can remain bipotential up to its final mitotic division. The possibility that these two cell types may also arise from a mitotic precursor during HC regeneration in the mature basilar papilla is consistent with their developmental history.
Key words: inner ear; basilar papilla; cochlea; ear development; cell lineage; sensory cell; retrovirus
Copyright © 1998 Society for Neuroscience 0270-6474/98/18197811-11$05.00/0
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