 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, October 1, 1998, 18(19):7856-7868
Spinal Cord Neuronal Precursors Generate Multiple Neuronal Phenotypes in Culture
Anjali J. Kalyani2, David Piper1, Tahmina Mujtaba2, Mary T. Lucero1, and Mahendra S. Rao2 1 Department of Physiology, University of Utah, Salt Lake City, Utah 84132, and 2 Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah 84132
Neuronal restricted precursors (NRPs) () can generate multiple neurotransmitter phenotypes during maturation in culture. Undifferentiated E-NCAM+ (embryonic neural cell adhesion molecule) immunoreactive NRPs are mitotically active and electrically immature, and they express only a subset of neuronal markers. Fully mature cells are postmitotic, process-bearing cells that are neurofilament-M and synaptophysin immunoreactive, and they synthesize and respond to different subsets of neurotransmitter molecules. Mature neurons that synthesize and respond to glycine, glutamate, GABA, dopamine, and acetylcholine can be identified by immunocytochemistry, RT-PCR, and calcium imaging in mass cultures. Individual NRPs also generate heterogeneous progeny as assessed by neurotransmitter response and synthesis, demonstrating the multipotent nature of the precursor cells.
Differentiation can be modulated by sonic hedgehog (Shh) and bone morphogenetic protein (BMP)-2/4 molecules. Shh acts as a mitogen and inhibits differentiation (including cholinergic differentiation). BMP-2 and BMP-4, in contrast, inhibit cell division and promote differentiation (including cholinergic differentiation). Thus, a single neuronal precursor cell can differentiate into multiple classes of neurons, and this differentiation can be modulated by environmental signals.
Key words: E-NCAM; spinal cord development; neuroblasts; stem cells; Shh; BMP
Copyright © 1998 Society for Neuroscience 0270-6474/98/18197856-13$05.00/0
This article has been cited by other articles:
C. Fekete, B. C. G. Freitas, A. Zeold, G. Wittmann, A. Kadar, Z. Liposits, M. A. Christoffolete, P. Singru, R. M. Lechan, A. C. Bianco, et al.
Expression Patterns of WSB-1 and USP-33 Underlie Cell-Specific Posttranslational Control of Type 2 Deiodinase in the Rat Brain
Endocrinology, October 1, 2007; 148(10): 4865 - 4874.
[Abstract] [Full Text] [PDF]
S. E. Kendall, C. Battelli, S. Irwin, J. G. Mitchell, C. A. Glackin, and J. M. Verdi
NRAGE Mediates p38 Activation and Neural Progenitor Apoptosis via the Bone Morphogenetic Protein Signaling Cascade
Mol. Cell. Biol., September 1, 2005; 25(17): 7711 - 7724.
[Abstract] [Full Text] [PDF]
A. Moshiri and T. A. Reh
Persistent Progenitors at the Retinal Margin of ptc+/- Mice
J. Neurosci., January 7, 2004; 24(1): 229 - 237.
[Abstract] [Full Text] [PDF]
G. Marques, T. E. Haerry, M. L. Crotty, M. Xue, B. Zhang, and M. B. O'Connor
Retrograde Gbb signaling through the Bmp type 2 receptor Wishful Thinking regulates systemic FMRFa expression in Drosophila
Development, November 15, 2003; 130(22): 5457 - 5470.
[Abstract] [Full Text] [PDF]
A. Gulacsi and L. Lillien
Sonic Hedgehog and Bone Morphogenetic Protein Regulate Interneuron Development from Dorsal Telencephalic Progenitors In Vitro
J. Neurosci., October 29, 2003; 23(30): 9862 - 9872.
[Abstract] [Full Text] [PDF]
C. C. Hegg, E. Au, A. J. Roskams, and M. T. Lucero
PACAP Is Present in the Olfactory System and Evokes Calcium Transients in Olfactory Receptor Neurons
J Neurophysiol, October 1, 2003; 90(4): 2711 - 2719.
[Abstract] [Full Text] [PDF]
M. Nagano, B.-Y. Ryu, C. J. Brinster, M. R. Avarbock, and R. L. Brinster
Maintenance of Mouse Male Germ Line Stem Cells In Vitro
Biol Reprod, June 1, 2003; 68(6): 2207 - 2214.
[Abstract] [Full Text] [PDF]
S L Preston, M R Alison, S J Forbes, N C Direkze, R Poulsom, and N A Wright
The new stem cell biology: something for everyone
Mol. Pathol., April 1, 2003; 56(2): 86 - 96.
[Abstract] [Full Text] [PDF]
A. M. Kenney, M. D. Cole, and D. H. Rowitch
Nmyc upregulation by sonic hedgehog signaling promotes proliferation in developing cerebellar granule neuron precursors
Development, January 1, 2003; 130(1): 15 - 28.
[Abstract] [Full Text] [PDF]
M. Zirlinger, L. Lo, J. McMahon, A. P. McMahon, and D. J. Anderson
Transient expression of the bHLH factor neurogenin-2 marks a subpopulation of neural crest cells biased for a sensory but not a neuronal fate
PNAS, June 11, 2002; 99(12): 8084 - 8089.
[Abstract] [Full Text] [PDF]
S. K. Mistry, E. W. Keefer, B. A. Cunningham, G. M. Edelman, and K. L. Crossin
Cultured rat hippocampal neural progenitors generate spontaneously active neural networks
PNAS, January 24, 2002; (2002) 22646599.
[Abstract] [Full Text] [PDF]
J. Lu, F. Feron, A. Mackay-Sim, and P. M. E. Waite
Olfactory ensheathing cells promote locomotor recovery after delayed transplantation into transected spinal cord
Brain, January 1, 2002; 125(1): 14 - 21.
[Abstract] [Full Text] [PDF]
S.-i. Yamamoto, M. Nagao, M. Sugimori, H. Kosako, H. Nakatomi, N. Yamamoto, H. Takebayashi, Y.-i. Nabeshima, T. Kitamura, G. Weinmaster, et al.
Transcription Factor Expression and Notch-Dependent Regulation of Neural Progenitors in the Adult Rat Spinal Cord
J. Neurosci., December 15, 2001; 21(24): 9814 - 9823.
[Abstract] [Full Text] [PDF]
N. Dahmane, P. Sanchez, Y. Gitton, V. Palma, T. Sun, M. Beyna, H. Weiner, and A. Ruiz i Altaba
The Sonic Hedgehog-Gli pathway regulates dorsal brain growth and tumorigenesis
Development, December 15, 2001; 128(24): 5201 - 5212.
[Abstract] [Full Text] [PDF]
D. M. Panchision, J. M. Pickel, L. Studer, S.-H. Lee, P. A. Turner, T. G. Hazel, and R. D.G. McKay
Sequential actions of BMP receptors control neural precursor cell production and fate
Genes & Dev., August 15, 2001; 15(16): 2094 - 2110.
[Abstract] [Full Text] [PDF]
H. Yang, T. Mujtaba, G. Venkatraman, Y. Y. Wu, M. S. Rao, and M. B. Luskin
Region-specific differentiation of neural tube-derived neuronal restricted progenitor cells after heterotopic transplantation
PNAS, November 21, 2000; 97(24): 13366 - 13371.
[Abstract] [Full Text] [PDF]
F. M. Vaccarino
Stem Cells and Neuronal Progenitors and Their Diversity in the CNS: Are Time and Place Important?
Neuroscientist, October 1, 2000; 6(5): 338 - 352.
[Abstract] [PDF]
I. López-Coviella, B. Berse, R. Krauss, R. S. Thies, and J. K. Blusztajn
Induction and Maintenance of the Neuronal Cholinergic Phenotype in the Central Nervous System by BMP-9
Science, July 14, 2000; 289(5477): 313 - 316.
[Abstract] [Full Text]
D. R. Piper, T. Mujtaba, M. S. Rao, and M. T. Lucero
Immunocytochemical and Physiological Characterization of a Population of Cultured Human Neural Precursors
J Neurophysiol, July 1, 2000; 84(1): 534 - 548.
[Abstract] [Full Text] [PDF]
S. S. W. Han and I. Fischer
Neural Stem Cells and Gene Therapy: Prospects for Repairing the Injured Spinal Cord
JAMA, May 3, 2000; 283(17): 2300 - 2301.
[Full Text] [PDF]
S Pons and E Marti
Sonic hedgehog synergizes with the extracellular matrix protein vitronectin to induce spinal motor neuron differentiation
Development, January 1, 2000; 127(2): 333 - 342.
[Abstract] [PDF]
D. H. Rowitch, B. St.-Jacques, S. M. K. Lee, J. D. Flax, E. Y. Snyder, and A. P. McMahon
Sonic hedgehog Regulates Proliferation and Inhibits Differentiation of CNS Precursor Cells
J. Neurosci., October 15, 1999; 19(20): 8954 - 8965.
[Abstract] [Full Text] [PDF]
P. C. Mabie, M. F. Mehler, and J. A. Kessler
Multiple Roles of Bone Morphogenetic Protein Signaling in the Regulation of Cortical Cell Number and Phenotype
J. Neurosci., August 15, 1999; 19(16): 7077 - 7088.
[Abstract] [Full Text] [PDF]
D. Orentas, J. Hayes, K. Dyer, and R. Miller
Sonic hedgehog signaling is required during the appearance of spinal cord oligodendrocyte precursors
Development, January 6, 1999; 126(11): 2419 - 2429.
[Abstract] [PDF]
S. K. Mistry, E. W. Keefer, B. A. Cunningham, G. M. Edelman, and K. L. Crossin
Cultured rat hippocampal neural progenitors generate spontaneously active neural networks
PNAS, February 5, 2002; 99(3): 1621 - 1626.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|