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The Journal of Neuroscience, October 1, 1998, 18(19):7891-7902
Promyelinating Schwann Cells Express Tst-1/SCIP/Oct-6
Edgardo J.
Arroyo1,
John R.
Bermingham Jr2,
Michael G.
Rosenfeld2, and
Steven S.
Scherer3
Departments of 1 Neuroscience and
3 Neurology, The University of Pennsylvania Medical Center,
Philadelphia, Pennsylvania 19104-6077, and 2 Howard Hughes
Medical Institute, Department of Medicine, University of
California, San Diego, La Jolla, California 92093-0623
Tst-1/SCIP/Oct-6, a POU domain transcription factor,
is transiently expressed by developing Schwann cells and is required for their normal development into a myelinating phenotype. In tst-1/scip/oct-6-null sciatic nerves, Schwann cells are
transiently arrested at the "promyelinating" stage, when they have
a one-to-one relationship with an axon but before they have elaborated
a myelin sheath. To determine when Schwann cells express
Tst-1/SCIP/Oct-6, we examined  -galactosidase (-gal) expression in
heterozygous tst-1/scip/oct-6 mice, in which one copy of
the tst-1/scip/oct-6 gene has been replaced with the
LacZ gene. -Gal expression from the LacZ gene seems to parallel
Tst-1/SCIP/Oct-6 expression from the endogenous
tst-1/scip/oct-6 gene in developing and regenerating sciatic nerves. Furthermore, electron microscopic examination of
5bromo-4-chloro-3-indolyl--D-galactopyranoside-
(X-gal) and halogenated indolyl--D-galactoside-
(Bluo-gal) stained nerves showed that promyelinating Schwann
cells express the highest levels of -gal, both in developing and in
regenerating nerves. Thus, the expression of -gal, a surrogate
marker of Tst-1/SCIP/Oct-6, peaks at the same stage of Schwann cell
development at which development is arrested in
tst-1/scip/oct-6-null mice, indicating that
Tst-1/SCIP/Oct-6 has a critical role in promyelinating Schwann
cells.
Key words:
myelin; transcription factors; cAMP; POU; axon-Schwann
cell interactions; peripheral nerve; neuropathy
Copyright © 1998 Society for Neuroscience 0270-6474/98/18197891-12$05.00/0
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