 |
||||
|
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, January 15, 1998, 18(2):625-633
Assembly of Proteins to Postsynaptic Densities after Transient Cerebral Ischemia
Bing-Ren Hu1, Minkyu Park2, Maryann E. Martone1, Wolfgang H. Fischer2, Mark H. Ellisman1, and Justin A. Zivin1 1 Department of Neuroscience, National Center for Microscopy and Imaging Research at San Diego, and Veterans Affairs Medical Center at San Diego, University of California, San Diego, La Jolla, California 92093-0624, and 2 The Clayton Foundation Laboratories for Peptide Biology, The Salk Institute, La Jolla, California 92037
Transient ischemia leads to changes in synaptic efficacy and results in selective neuronal damage during the postischemic phase, although the mechanisms are not fully understood. The protein composition and ultrastructure of postsynaptic densities (PSDs) were studied by using a rat transient ischemic model. We found that a brief ischemic episode induced a marked accumulation in PSDs of the protein assembly ATPases, N-ethylmaleimide-sensitive fusion protein, and heat-shock cognate protein-70 as well as the BDNF receptor (trkB) and protein kinases, as determined by protein microsequencing. The changes in PSD composition were accompanied by a 2.5-fold increase in the yield of PSD protein relative to controls. Biochemical modification of PSDs correlated well with an increase in PSD thickness observed in vivo by electron microscopy. We conclude that a brief ischemic episode modifies the molecular composition and ultrastructure of synapses by assembly of proteins to the postsynaptic density, which may underlie observed changes in synaptic function and selective neuronal damage.
Key words: postsynaptic density; synaptic plasticity; protein kinases; microsequencing; cerebral ischemia; electron microscope; neuronal damage
Copyright © 1998 Society for Neuroscience 0270-6474/98/182625-09$05.00/0
This article has been cited by other articles:
X. Xu, L. Ye, and Q. Ruan
Environmental Enrichment Induces Synaptic Structural Modification After Transient Focal Cerebral Ischemia in Rats
Experimental Biology and Medicine, March 1, 2009; 234(3): 296 - 305.
[Abstract] [Full Text] [PDF]
P. K. Shetty, F. L. Huang, and K.-P. Huang
Ischemia-elicited Oxidative Modulation of Ca2+/Calmodulin-dependent Protein Kinase II
J. Biol. Chem., February 29, 2008; 283(9): 5389 - 5401.
[Abstract] [Full Text] [PDF]
X. Chen, L. Vinade, R. D. Leapman, J. D. Petersen, T. Nakagawa, T. M. Phillips, M. Sheng, and T. S. Reese
Mass of the postsynaptic density and enumeration of three key molecules
PNAS, August 9, 2005; 102(32): 11551 - 11556.
[Abstract] [Full Text] [PDF]
H. Bolay, Y. Gursoy-Ozdemir, Y. Sara, R. Onur, A. Can, and T. Dalkara
Persistent Defect in Transmitter Release and Synapsin Phosphorylation in Cerebral Cortex After Transient Moderate Ischemic Injury
Stroke, May 1, 2002; 33(5): 1369 - 1375.
[Abstract] [Full Text] [PDF]
A. Dosemeci, J.-H. Tao-Cheng, L. Vinade, C. A. Winters, L. Pozzo-Miller, and T. S. Reese
Glutamate-induced transient modification of the postsynaptic density
PNAS, August 17, 2001; (2001) 181336998.
[Abstract] [Full Text] [PDF]
G.-Y. Liao, D. A. Wagner, M. H. Hsu, and J. P. Leonard
Evidence for Direct Protein Kinase-C Mediated Modulation of N-Methyl-D-aspartate Receptor Current
Mol. Pharmacol., April 16, 2001; 59(5): 960 - 964.
[Abstract] [Full Text]
T. Opitz, S. Y. Grooms, M. V. L. Bennett, and R. S. Zukin
Remodeling of alpha -amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor subunit composition in hippocampal neurons after global ischemia
PNAS, November 21, 2000; 97(24): 13360 - 13365.
[Abstract] [Full Text] [PDF]
A. Dosemeci, T. S. Reese, J. Petersen, and J.-H. Tao-Cheng
A Novel Particulate Form of Ca2+/CaMKII-Dependent Protein Kinase II in Neurons
J. Neurosci., May 1, 2000; 20(9): 3076 - 3084.
[Abstract] [Full Text] [PDF]
B. R. Hu, M. E. Martone, Y. Z. Jones, and C. L. Liu
Protein Aggregation after Transient Cerebral Ischemia
J. Neurosci., May 1, 2000; 20(9): 3191 - 3199.
[Abstract] [Full Text] [PDF]
T. L. Briones and B. Therrien
Behavioral Effects of Transient Cerebral Ischemia
Biol Res Nurs, April 1, 2000; 1(4): 276 - 286.
[Abstract] [PDF]
T. L. Briones, B. Therrien, and B. Metzger
Effects of Environment on Enhancing Functional Plasticity following Cerebral Ischemia
Biol Res Nurs, April 1, 2000; 1(4): 299 - 309.
[Abstract] [PDF]
M. J. Hasbani, S. M. Underhill, G. De Erausquin, and M. P. Goldberg
Synapse Loss and Regeneration: A Mechanism for Functional Decline and Recovery after Cerebral Ischemia?
Neuroscientist, April 1, 2000; 6(2): 110 - 119.
[Abstract] [PDF]
A. Ouanonou, Y. Zhang, and L. Zhang
Changes in the Calcium Dependence of Glutamate Transmission in the Hippocampal CA1 Region After Brief Hypoxia-Hypoglycemia
J Neurophysiol, September 1, 1999; 82(3): 1147 - 1155.
[Abstract] [Full Text] [PDF]
S. A. Kirov, K. E. Sorra, and K. M. Harris
Slices Have More Synapses than Perfusion-Fixed Hippocampus from both Young and Mature Rats
J. Neurosci., April 15, 1999; 19(8): 2876 - 2886.
[Abstract] [Full Text] [PDF]
M. E. Martone, Y. Z. Jones, S. J. Young, M. H. Ellisman, J. A. Zivin, and B.-R. Hu
Modification of Postsynaptic Densities after Transient Cerebral Ischemia: A Quantitative and Three-Dimensional Ultrastructural Study
J. Neurosci., March 15, 1999; 19(6): 1988 - 1997.
[Abstract] [Full Text] [PDF]
K. -H. Smalla, H. Matthies, K. Langnase, S. Shabir, T. M. Bockers, U. Wyneken, S. Staak, M. Krug, P. W. Beesley, and E. D. Gundelfinger
The synaptic glycoprotein neuroplastin is involved in long-term potentiation at hippocampal CA1 synapses
PNAS, April 11, 2000; 97(8): 4327 - 4332.
[Abstract] [Full Text] [PDF]
A. Dosemeci, J.-H. Tao-Cheng, L. Vinade, C. A. Winters, L. Pozzo-Miller, and T. S. Reese
Glutamate-induced transient modification of the postsynaptic density
PNAS, August 28, 2001; 98(18): 10428 - 10432.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|