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Previous Article | Next Article
The Journal of Neuroscience, January 15, 1998, 18(2):741-750
Drosophila Photoreceptors Contain an Autonomous Circadian Oscillator That Can Function without period mRNA Cycling
Yuzhong Cheng1 and Paul E. Hardin2 1 Department of Biology, Texas A & M University, College Station, Texas 77843, and 2 Department of Biology, University of Houston, Houston, Texas 77204
Circadian oscillations in period (per) mRNA and per protein (PER) constitute, in part, a feedback loop that is required for circadian pacemaker function in Drosophila melanogaster. Oscillations in PER are required for oscillations in per mRNA, but the converse has not been rigorously tested because of a lack of measurable quantities of per mRNA and protein in the same cells. This circadian feedback loop operates synchronously in many neuronal and non-neuronal tissues, including a set of lateral brain neurons (LNs) that mediate rhythms in locomotor activity, but whether a hierarchy among these tissues maintains this synchrony is not known. To determine whether per mRNA cycling is necessary for PER cycling and whether cyclic per gene expression is tissue autonomous, we have generated per01 flies carrying a transgene that constitutively expresses per mRNA specifically in photoreceptors, a cell type that supports feedback loop function. These transformants were tested for different aspects of feedback loop function including per mRNA cycling, PER cycling, and PER nuclear localization. Under both light/dark (LD) cycling and constant dark (DD) conditions, PER abundance cycles in the absence of circadian cycling of per mRNA. These results show that per mRNA cycling is not required for PER cycling and indicate that Drosophila photoreceptors R1-R6 contain a tissue autonomous circadian oscillator.
Key words: Drosophila; circadian clock; photoreceptors; period gene; transgene; gene expression
Copyright © 1998 Society for Neuroscience 0270-6474/98/182741-10$05.00/0
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