 |
 Previous Article | Next Article 
The Journal of Neuroscience, October 15, 1998, 18(20):8198-8213
Two Distinct Nicotinic Receptors, One Pharmacologically Similar
to the Vertebrate  7-Containing Receptor, Mediate Cl Currents in
Aplysia Neurons
JacSue
Kehoe1 and
J.
Michael
McIntosh2
1 Laboratoire de Neurobiologie, Ecole Normale
Supérieure, Paris 75005, France, and 2 Departments of
Psychiatry and Biology, University of Utah, Salt Lake City, Utah 84112
Ionotropic, nicotinic receptors have previously been shown to
mediate both inhibitory (Cl-dependent) and excitatory (cationic) cholinergic responses in Aplysia neurons. We have used
fast perfusion methods of agonist and antagonist application to
reevaluate the effects on these receptors of a wide variety of
cholinergic compounds, including a number of recently isolated and/or
synthesized  toxins [-conotoxin (CTx)] from
Conus snails. These toxins have been shown in previous
studies to discriminate between the many types of nicotinic receptors
now known to be expressed in vertebrate muscle, neuroendocrine, and
neuronal cells. One of these toxins (CTx ImI from the
worm-eating snail Conus imperialis) revealed that two
kinetically and pharmacologically distinct elements underlie the
ACh-induced Cl-dependent response in Aplysia neurons:
one element is a rapidly desensitizing current that is blocked by the
toxin; the other is a slowly desensitizing current that is unaffected
by the toxin. The two kinetically defined elements were also found to
be differentially sensitive to different agonists. Finally, the
proportion of the rapidly desensitizing element to the sustained
element was found to be cell-specific. These observations led to the
conclusion that two distinct nicotinic receptors mediate Cl currents in
Aplysia neurons. The receptor mediating the rapidly desensitizing Cl-dependent response shows a strong pharmacological resemblance to the vertebrate -bungarotoxin-sensitive,
7-containing receptor, which is permeable to calcium and mediates a
rapidly desensitizing excitatory response.
Key words:
nicotinic receptor; acetylcholine; 7; chloride; Aplysia; -conotoxin ImI; suberyldicholine; methyllycaconitine; -bungarotoxin; strychnine; dihydro- -erythroidine
Copyright © 1998 Society for Neuroscience 0270-6474/98/18208198-16$05.00/0
This article has been cited by other articles:
|
|
|
|
 
A. Khoutorsky and M. E. Spira
Activity-dependent calpain activation plays a critical role in synaptic facilitation and post-tetanic potentiation
Learn. Mem.,
January 29, 2009;
16(2):
129 - 141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. B. Hansen, H.-L. Wang, P. Taylor, and S. M. Sine
An Ion Selectivity Filter in the Extracellular Domain of Cys-loop Receptors Reveals Determinants for Ion Conductance
J. Biol. Chem.,
December 26, 2008;
283(52):
36066 - 36070.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. T. Talley, B. M. Olivera, K.-H. Han, S. B. Christensen, C. Dowell, I. Tsigelny, K.-Y. Ho, P. Taylor, and J. M. McIntosh
{alpha}-Conotoxin OmIA Is a Potent Ligand for the Acetylcholine-binding Protein as Well as {alpha}3beta2 and {alpha}7 Nicotinic Acetylcholine Receptors
J. Biol. Chem.,
August 25, 2006;
281(34):
24678 - 24686.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. van Nierop, A. Keramidas, S. Bertrand, J. van Minnen, Y. Gouwenberg, D. Bertrand, and A. B. Smit
Identification of Molluscan Nicotinic Acetylcholine Receptor (nAChR) Subunits Involved in Formation of Cation- and Anion-Selective nAChRs
J. Neurosci.,
November 16, 2005;
25(46):
10617 - 10626.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Schnizler, B. Saeger, C. Pfeffer, A. Gerbaulet, U. Ebbinghaus-Kintscher, C. Methfessel, E.-M. Franken, K. Raming, C. H. Wetzel, A. Saras, et al.
A Novel Chloride Channel in Drosophila melanogaster Is Inhibited by Protons
J. Biol. Chem.,
April 22, 2005;
280(16):
16254 - 16262.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Putrenko, M. Zakikhani, and J. A. Dent
A Family of Acetylcholine-gated Chloride Channel Subunits in Caenorhabditis elegans
J. Biol. Chem.,
February 25, 2005;
280(8):
6392 - 6398.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. TERLAU and B. M. OLIVERA
Conus Venoms: A Rich Source of Novel Ion Channel-Targeted Peptides
Physiol Rev,
January 1, 2004;
84(1):
41 - 68.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Hunter and J. G. Milton
Amplitude and Frequency Dependence of Spike Timing: Implications for Dynamic Regulation
J Neurophysiol,
July 1, 2003;
90(1):
387 - 394.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Humeau, M. R. Popoff, H. Kojima, F. Doussau, and B. Poulain
Rac GTPase Plays an Essential Role in Exocytosis by Controlling the Fusion Competence of Release Sites
J. Neurosci.,
September 15, 2002;
22(18):
7968 - 7981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Dilger
The effects of general anaesthetics on ligand-gated ion channels
Br. J. Anaesth.,
July 1, 2002;
89(1):
41 - 51.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Woodin, D. W. Munno, and N. I. Syed
Trophic Factor-Induced Excitatory Synaptogenesis Involves Postsynaptic Modulation of Nicotinic Acetylcholine Receptors
J. Neurosci.,
January 15, 2002;
22(2):
505 - 514.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Ohnuma, M. D Whim, R. D Fetter, L. K Kaczmarek, and R. S Zucker
Presynaptic target of Ca2+ action on neuropeptide and acetylcholine release in Aplysia californica
J. Physiol.,
September 15, 2001;
535(3):
647 - 662.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Hunter and J. G. Milton
Synaptic Heterogeneity and Stimulus-Induced Modulation of Depression in Central Synapses
J. Neurosci.,
August 1, 2001;
21(15):
5781 - 5793.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Courjaret and B. Lapied
Complex Intracellular Messenger Pathways Regulate One Type of Neuronal alpha -Bungarotoxin-Resistant Nicotinic Acetylcholine Receptors Expressed in Insect Neurosecretory Cells (Dorsal Unpaired Median Neurons)
Mol. Pharmacol.,
July 1, 2001;
60(1):
80 - 91.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. L. Tieman, D. J. Steel, Y. Gor, J. Kehoe, J. H. Schwartz, and S. J. Feinmark
A Pertussis Toxin-Sensitive 8-Lipoxygenase Pathway Is Activated by a Nicotinic Acetylcholine Receptor in Aplysia Neurons
J Neurophysiol,
May 1, 2001;
85(5):
2150 - 2158.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Devlin
The pharmacology of gamma-aminobutyric acid and acetylcholine receptors at the echinoderm neuromuscular junction
J. Exp. Biol.,
January 3, 2001;
204(5):
887 - 896.
[Abstract]
[PDF]
|
|
|
|
|
|
|
H. Matzner, Y. Gutfreund, and B. Hochner
Neuromuscular System of the Flexible Arm of the Octopus: Physiological Characterization
J Neurophysiol,
March 1, 2000;
83(3):
1315 - 1328.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
