QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (45) Citing Articles via Google Scholar Google Scholar Articles by Lambert, R. C. Articles by Feltz, A. Search for Related Content PubMed PubMed Citation Articles by Lambert, R. C. Articles by Feltz, A.

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 1, 1998, 18(21):8605-8613

Low-Voltage-Activated Ca²⁺ Currents Are Generated by Members of the Ca_vT Subunit Family (1G/H) in Rat Primary Sensory Neurons

Régis C. Lambert¹, Frank McKenna¹, Yves Maulet¹, Edmund M. Talley², Douglas A. Bayliss², Leanne L. Cribbs³, Jung-Ha Lee³, Edward Perez-Reyes³, and Anne Feltz¹

¹ Laboratoire de Neurobiologie Cellulaire, UPR 9009-Centre National de la Recherche Scientifique, F-67084, Strasbourg, France, ² Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, and ³ Loyola University Medical Center, Maywood, Illinois 60153

Recently, two members of a new family of Ca²⁺ channel 1 subunits, 1G (or Ca_vT.1) and 1H (or Ca_vT.2), have been cloned and expressed. These 1 subunits generate Ba²⁺ currents similar to the T-type Ca²⁺ currents present in sensory neurons. Here, we use three methods to investigate whether the T currents of nodosus ganglion neurons are encoded by members of the Ca_vT family. PCR detected the presence of mRNA encoding both 1G and 1H, as well as a third highly related sequence, 1I. In situ hybridizations performed on nodosus ganglia demonstrate a high expression of 1H subunit RNAs. Transfection of nodosus ganglion neurons with a generic antisense oligonucleotide against this new 1 subunit family selectively suppresses the low-voltage-activated Ca²⁺ current. The antisense oligonucleotide effect increased with time after transfection and reached a maximum 3 d after treatment, indicating a 2-3 d turnover for the 1 proteins. Taken together, these results suggest that the T-type current present in the sensory neurons is mainly attributable to 1H channels. In addition, taking advantage of the high specificity of the antisense ON to the cloned channels, we showed that T-type currents greatly slowed the repolarization occurring during an action potential and were responsible for up to 51% of the Ca²⁺ entry during spikes. Therefore, the antisense strategy clearly demonstrates the role of low-voltage-activated Ca²⁺ current in affecting the afterpotential properties and influencing the cell excitability. Such tools should be beneficial to further studies investigating physiological roles of T-type Ca²⁺ currents.

Key words: low-threshold/T-type calcium channels; antisense oligonucleotides; PCR; in situ hybridization; action potentials; sensory neurons; nodosus ganglion

---

Copyright © 1998 Society for Neuroscience  0270-6474/98/18218605-09$05.00/0

This article has been cited by other articles:

				Z. Fang, C.-K. Park, H. Y. Li, H. Y. Kim, S.-H. Park, S. J. Jung, J. S. Kim, A. Monteil, S. B. Oh, and R. J. Miller Molecular Basis of Cav2.3 Calcium Channels in Rat Nociceptive Neurons J. Biol. Chem., February 16, 2007; 282(7): 4757 - 4764. [Abstract] [Full Text] [PDF]

				A. SPAT and L. HUNYADY Control of Aldosterone Secretion: A Model for Convergence in Cellular Signaling Pathways Physiol Rev, April 1, 2004; 84(2): 489 - 539. [Abstract] [Full Text] [PDF]

				S. C. Lee, Y. Hayashida, and A. T. Ishida Availability of Low-Threshold Ca2+ Current in Retinal Ganglion Cells J Neurophysiol, December 1, 2003; 90(6): 3888 - 3901. [Abstract] [Full Text] [PDF]

				J.-H. Lee, E.-G. Kim, B.-G. Park, K.-H. Kim, S.-K. Cha, I. D. Kong, J.-W. Lee, and S.-W. Jeong Identification of T-Type alpha 1H Ca2+ Channels (Cav3.2) in Major Pelvic Ganglion Neurons J Neurophysiol, June 1, 2002; 87(6): 2844 - 2850. [Abstract] [Full Text] [PDF]

				S. S. Sidach and I. M. Mintz Kurtoxin, A Gating Modifier of Neuronal High- and Low-Threshold Ca Channels J. Neurosci., March 15, 2002; 22(6): 2023 - 2034. [Abstract] [Full Text] [PDF]

				S. M. Todorovic, V. Jevtovic-Todorovic, S. Mennerick, E. Perez-Reyes, and C. F. Zorumski Cav3.2 Channel Is a Molecular Substrate for Inhibition of T-Type Calcium Currents in Rat Sensory Neurons by Nitrous Oxide Mol. Pharmacol., September 1, 2001; 60(3): 603 - 610. [Abstract] [Full Text] [PDF]

				L. L. Cribbs, B. L. Martin, E. A. Schroder, B. B. Keller, B. P. Delisle, and J. Satin Identification of the T-Type Calcium Channel (CaV3.1d) in Developing Mouse Heart Circ. Res., March 2, 2001; 88(4): 403 - 407. [Abstract] [Full Text] [PDF]

				W.-Y. Son, J.-H. Lee, J.-H. Lee, and C.-T. Han Acrosome reaction of human spermatozoa is mainly mediated by {alpha}1H T-type calcium channels Mol. Hum. Reprod., October 1, 2000; 6(10): 893 - 897. [Abstract] [Full Text] [PDF]

				J. Satin and L. L. Cribbs Identification of a T-Type Ca2+ Channel Isoform in Murine Atrial Myocytes (AT-1 Cells) Circ. Res., March 31, 2000; 86(6): 636 - 642. [Abstract] [Full Text] [PDF]

				E. M. Talley, L. L. Cribbs, J.-H. Lee, A. Daud, E. Perez-Reyes, and D. A. Bayliss Differential Distribution of Three Members of a Gene Family Encoding Low Voltage-Activated (T-Type) Calcium Channels J. Neurosci., March 15, 1999; 19(6): 1895 - 1911. [Abstract] [Full Text] [PDF]

				G. Wennemuth, R. E. Westenbroek, T. Xu, B. Hille, and D. F. Babcock CaV2.2 and CaV2.3 (N- and R-type) Ca2+ Channels in Depolarization-evoked Entry of Ca2+ into Mouse Sperm J. Biol. Chem., July 7, 2000; 275(28): 21210 - 21217. [Abstract] [Full Text] [PDF]

				J. Chemin, A. Monteil, E. Perez-Reyes, E. Bourinet, J. Nargeot, and P. Lory Specific contribution of human T-type calcium channel isotypes (alpha1G, alpha1H and alpha1I) to neuronal excitability J. Physiol., April 1, 2002; 540(1): 3 - 14. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help