 |
||||
|
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, November 1, 1998, 18(21):8886-8899
The Steroid Hormone 20-Hydroxyecdysone Enhances Neurite Growth of Drosophila Mushroom Body Neurons Isolated during Metamorphosis
Robert Kraft, Richard B. Levine, and Linda L. Restifo Arizona Research Laboratories, Division of Neurobiology, University of Arizona, Tucson, Arizona 85721-0077
Mushroom bodies (MBs) are symmetrically paired neuropils in the insect brain that are of critical importance for associative olfactory learning and memory. In Drosophila melanogaster, the MB intrinsic neurons (Kenyon cells) undergo extensive reorganization at the onset of metamorphosis. A phase of rapid axonal degeneration without cell death is followed by axonal regeneration. This re-elaboration occurs as levels of the steroid hormone 20-hydroxyecdysone (20E) are rising during the pupal stage. Based on the known role of 20E in directing many features of CNS remodeling during insect metamorphosis, we hypothesized that the outgrowth of MB axonal processes is promoted by 20E. Using a GAL4 enhancer trap line (201Y) that drives MB-restricted reporter gene expression, we identified Kenyon cells in primary cultures dissociated from early pupal CNS. Paired cultures derived from single brains isolated before the 20E pupal peak were incubated in medium with or without 20E for 2-4 d. Morphometric analysis demonstrated that MB neurons exposed to 20E had significantly greater total neurite length and branch number compared with that of MB neurons grown without hormone. The relationship between branch number and total neurite length remained constant regardless of hormone treatment in vitro, suggesting that 20E enhances the rate of outgrowth from pupal MB neurons in a proportionate manner and does not selectively increase neuritic branching. These results implicate 20E in enhancing axonal outgrowth of Kenyon cells to support MB remodeling during metamorphosis.
Key words: steroid hormone; ecdysteroids; metamorphosis; Drosophila melanogaster; polarity; mushroom body; Kenyon cells; neuronal remodeling; neurite outgrowth; cell culture
Copyright © 1998 Society for Neuroscience 0270-6474/98/18218886-14$05.00/0
This article has been cited by other articles:
H. Ishimoto, T. Sakai, and T. Kitamoto
Ecdysone signaling regulates the formation of long-term courtship memory in adult Drosophila melanogaster
PNAS, April 14, 2009; 106(15): 6381 - 6386.
[Abstract] [Full Text] [PDF]
R. Kraft, M. M. Escobar, M. L. Narro, J. L. Kurtis, A. Efrat, K. Barnard, and L. L. Restifo
Phenotypes of Drosophila Brain Neurons in Primary Culture Reveal a Role for Fascin in Neurite Shape and Trajectory.
J. Neurosci., August 23, 2006; 26(34): 8734 - 8747.
[Abstract] [Full Text] [PDF]
Y.-J. Choi, G. Lee, and J. H. Park
Programmed cell death mechanisms of identifiable peptidergic neurons in Drosophila melanogaster
Development, June 1, 2006; 133(11): 2223 - 2232.
[Abstract] [Full Text] [PDF]
S. A. Jiang, J. M. Campusano, H. Su, and D. K. O'Dowd
Drosophila Mushroom Body Kenyon Cells Generate Spontaneous Calcium Transients Mediated by PLTX-Sensitive Calcium Channels
J Neurophysiol, July 1, 2005; 94(1): 491 - 500.
[Abstract] [Full Text] [PDF]
A. B. da Cruz, M. Schwarzel, S. Schulze, M. Niyyati, M. Heisenberg, and D. Kretzschmar
Disruption of the MAP1B-related Protein FUTSCH Leads to Changes in the Neuronal Cytoskeleton, Axonal Transport Defects, and Progressive Neurodegeneration in Drosophila
Mol. Biol. Cell, May 1, 2005; 16(5): 2433 - 2442.
[Abstract] [Full Text] [PDF]
S. Sanyal, C. Consoulas, H. Kuromi, A. Basole, L. Mukai, Y. Kidokoro, K. S. Krishnan, and M. Ramaswami
Analysis of Conditional Paralytic Mutants in Drosophila Sarco-Endoplasmic Reticulum Calcium ATPase Reveals Novel Mechanisms for Regulating Membrane Excitability
Genetics, February 1, 2005; 169(2): 737 - 750.
[Abstract] [Full Text] [PDF]
C. I. Michel, R. Kraft, and L. L. Restifo
Defective Neuronal Development in the Mushroom Bodies of Drosophila Fragile X Mental Retardation 1 Mutants
J. Neurosci., June 23, 2004; 24(25): 5798 - 5809.
[Abstract] [Full Text] [PDF]
C. Wegener, Y. Hamasaka, and D. R. Nassel
Acetylcholine Increases Intracellular Ca2+ Via Nicotinic Receptors in Cultured PDF-Containing Clock Neurons of Drosophila
J Neurophysiol, February 1, 2004; 91(2): 912 - 923.
[Abstract] [Full Text] [PDF]
J. K. Inlow and L. L. Restifo
Molecular and Comparative Genetics of Mental Retardation
Genetics, February 1, 2004; 166(2): 835 - 881.
[Abstract] [Full Text] [PDF]
H. Su and D. K. O'Dowd
Fast Synaptic Currents in Drosophila Mushroom Body Kenyon Cells Are Mediated by {alpha}-Bungarotoxin-Sensitive Nicotinic Acetylcholine Receptors and Picrotoxin-Sensitive GABA Receptors
J. Neurosci., October 8, 2003; 23(27): 9246 - 9253.
[Abstract] [Full Text] [PDF]
A. R. Rodan, J. A. Kiger Jr, and U. Heberlein
Functional Dissection of Neuroanatomical Loci Regulating Ethanol Sensitivity in Drosophila
J. Neurosci., November 1, 2002; 22(21): 9490 - 9501.
[Abstract] [Full Text] [PDF]
C. Consoulas, L. L. Restifo, and R. B. Levine
Dendritic Remodeling and Growth of Motoneurons during Metamorphosis of Drosophila melanogaster
J. Neurosci., June 15, 2002; 22(12): 4906 - 4917.
[Abstract] [Full Text] [PDF]
H. Li, D. Harrison, G. Jones, D. Jones, and R. L. Cooper
Alterations in Development, Behavior, and Physiology in Drosophila Larva That Have Reduced Ecdysone Production
J Neurophysiol, January 1, 2001; 85(1): 98 - 104.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|