WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (27)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Blaine, J. T.
Right arrow Articles by Ribera, A. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blaine, J. T.
Right arrow Articles by Ribera, A. B.

 Previous Article  |  Next Article 

The Journal of Neuroscience, December 1, 1998, 18(23):9585-9593

Heteromultimeric Potassium Channels Formed by Members of the Kv2 Subfamily

Judith T. Blaine and Angeles B. Ribera

Department of Physiology and Biophysics, University of Colorado Health Sciences Center, Denver, Colorado 80262

Four alpha -subunits are thought to coassemble and form a voltage-dependent potassium (Kv) channel. Kv alpha -subunits belong to one of four major subfamilies (Kv1, Kv2, Kv3, Kv4). Within a subfamily up to eight different genetic isotypes exist (e.g., Kv1.1, Kv1.2). Different isotypes within the Kv1 or Kv3 subfamily coassemble. It is not known, however, whether the only two members of the vertebrate Kv2 subfamily identified thus far, Kv2.1 and Kv2.2, heteromultimerize. This might account for the lack of detection of heteromultimeric Kv2 channels in situ despite the coexpression of Kv2.1 and Kv2.2 mRNAs within the same cell. To probe whether Kv2 isotypes can form heteromultimers, we developed a dominant-negative mutant Kv2.2 subunit to act as a molecular poison of Kv2 subunit-containing channels. The dominant-negative Kv2.2 suppresses formation of functional channels when it is coexpressed in oocytes with either wild-type Kv2.2 or Kv2.1 subunits. These results indicate that Kv2.1 and Kv2.2 subunits are capable of heteromultimerization. Thus, in native cells either Kv2.1 and Kv2.2 subunits are targeted at an early stage to different biosynthetic compartments or heteromultimerization otherwise is inhibited.

Key words: potassium channels; heteromultimers; coassembly; Kv2; dominant-negative mutant; Xenopus

Copyright © 1998 Society for Neuroscience  0270-6474/98/18239585-09$05.00/0


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
E. Bocksteins, A. L. Raes, G. Van de Vijver, T. Bruyns, P.-P. Van Bogaert, and D. J. Snyders
Kv2.1 and silent Kv subunits underlie the delayed rectifier K+ current in cultured small mouse DRG neurons
Am J Physiol Cell Physiol, June 1, 2009; 296(6): C1271 - C1278.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
H. Vacher, D. P. Mohapatra, and J. S. Trimmer
Localization and Targeting of Voltage-Dependent Ion Channels in Mammalian Central Neurons
Physiol Rev, October 1, 2008; 88(4): 1407 - 1447.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
D. Guan, T. Tkatch, D. J. Surmeier, W. E. Armstrong, and R. C. Foehring
Kv2 subunits underlie slowly inactivating potassium current in rat neocortical pyramidal neurons
J. Physiol., June 15, 2007; 581(3): 941 - 960.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. T. Blaine, A. D. Taylor, and A. B. Ribera
Carboxyl Tail Region of the Kv2.2 Subunit Mediates Novel Developmental Regulation of Channel Density
J Neurophysiol, December 1, 2004; 92(6): 3446 - 3454.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
L. Yan, D. J. Figueroa, C. P. Austin, Y. Liu, R. M. Bugianesi, R. S. Slaughter, G. J. Kaczorowski, and M. G. Kohler
Expression of Voltage-Gated Potassium Channels in Human and Rhesus Pancreatic Islets
Diabetes, March 1, 2004; 53(3): 597 - 607.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Pal, K. A. Hartnett, J. M. Nerbonne, E. S. Levitan, and E. Aizenman
Mediation of Neuronal Apoptosis by Kv2.1-Encoded Potassium Channels
J. Neurosci., June 15, 2003; 23(12): 4798 - 4802.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. A. Malin and J. M. Nerbonne
Delayed Rectifier K+ Currents, IK, Are Encoded by Kv2 alpha -Subunits and Regulate Tonic Firing in Mammalian Sympathetic Neurons
J. Neurosci., December 1, 2002; 22(23): 10094 - 10105.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
D. S Hogg, A. R.L Davies, G. McMurray, and R. Z Kozlowski
KV2.1 channels mediate hypoxic inhibition of IKV in native pulmonary arterial smooth muscle cells of the rat
Cardiovasc Res, August 1, 2002; 55(2): 349 - 360.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
P. Escoubas, S. Diochot, M.-L. Celerier, T. Nakajima, and M. Lazdunski
Novel Tarantula Toxins for Subtypes of Voltage-Dependent Potassium Channels in the Kv2 and Kv4 Subfamilies
Mol. Pharmacol., July 1, 2002; 62(1): 48 - 57.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Ottschytsch, A. Raes, D. Van Hoorick, and D. J. Snyders
Obligatory heterotetramerization of three previously uncharacterized Kv channel alpha -subunits identified in the human genome
PNAS, June 11, 2002; 99(12): 7986 - 7991.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
P. E. MacDonald, X. F. Ha, J. Wang, S. R. Smukler, A. M. Sun, H. Y. Gaisano, A. M. F. Salapatek, P. H. Backx, and M. B. Wheeler
Members of the Kv1 and Kv2 Voltage-Dependent K+ Channel Families Regulate Insulin Secretion
Mol. Endocrinol., August 1, 2001; 15(8): 1423 - 1435.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. T. Blaine and A. B. Ribera
Kv2 Channels Form Delayed-Rectifier Potassium Channels In Situ
J. Neurosci., March 1, 2001; 21(5): 1473 - 1480.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. A Wigmore and M. G Lacey
A Kv3-like persistent, outwardly rectifying, Cs+-permeable, K+ current in rat subthalamic nucleus neurones
J. Physiol., September 15, 2000; 527(3): 493 - 506.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. D. Chiara, F. Monje, A. Castellano, and J. Lopez-Barneo
A Small Domain in the N Terminus of the Regulatory alpha -Subunit Kv2.3 Modulates Kv2.1 Potassium Channel Gating
J. Neurosci., August 15, 1999; 19(16): 6865 - 6873.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
F. Ono, Y. Katsuyama, K. Nakajo, and Y. Okamura
Subfamily-Specific Posttranscriptional Mechanism Underlies K+ Channel Expression in a Developing Neuronal Blastomere
J. Neurosci., August 15, 1999; 19(16): 6874 - 6886.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
G. Baranauskas, T. Tkatch, and D. J. Surmeier
Delayed Rectifier Currents in Rat Globus Pallidus Neurons Are Attributable to Kv2.1 and Kv3.1/3.2 K+ Channels
J. Neurosci., August 1, 1999; 19(15): 6394 - 6404.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. A. Wielowieyski, J. T. Wigle, M. Salih, P. Hum, and B. S. Tuana
Alternative Splicing in Intracellular Loop Connecting Domains II and III of the alpha 1 Subunit of Cav1.2 Ca2+ Channels Predicts Two-domain Polypeptides with Unique C-terminal Tails
J. Biol. Chem., January 5, 2001; 276(2): 1398 - 1406.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2009 by Society for Neuroscience ONLINE ISSN: 1529-2401
-