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The Journal of Neuroscience, December 1, 1998, 18(23):9977-9988
Chemotopic, Combinatorial, and Noncombinatorial Odorant
Representations in the Olfactory Bulb Revealed Using a
Voltage-Sensitive Axon Tracer
Rainer W.
Friedrich1 and
Sigrun I.
Korsching2
1 Max-Planck-Institut für Entwicklungsbiologie,
Abteilung Physikalische Biologie, D-72076 Tübingen,
Germany, and 2 Institut für Genetik der
Universität zu Köln, D-50674 Köln, Germany
Odor information is first represented in the brain by patterns of
input activity across the glomeruli of the olfactory bulb (OB). To
examine how odorants are represented at this stage of olfactory
processing, we labeled anterogradely the axons of olfactory receptor
neurons with the voltage-sensitive dye Di8-ANEPPQ in zebrafish.
The activity induced by diverse natural odorants in afferent axons and
across the array of glomeruli was then recorded optically. The results
show that certain subregions of the OB are preferentially activated by
defined chemical odorant classes. Within these subregions,
"ordinary" odorants (amino acids, bile acids, and nucleotides)
induce overlapping activity patterns involving multiple glomeruli,
indicating that they are represented by combinatorial activity
patterns. In contrast, two putative pheromone components (prostaglandin
F2 and
17 ,20 -dihydroxy-4-pregnene-3-one-20-sulfate) each induce a single
focus of activity, at least one of which comes from a single, highly
specific and sensitive glomerulus. These results indicate that the OB
is organized into functional subregions processing classes of odorants.
Furthermore, they suggest that individual odorants can be represented
by "combinatorial" or "noncombinatorial" (focal)
activity patterns and that the latter may serve to process odorants
triggering distinct responses such as that of pheromones.
Key words:
olfactory coding; optical recording; axon tracing; activity pattern; zebrafish; pheromone; olfactory glomerulus; voltage-sensitive dye
Copyright © 1998 Society for Neuroscience 0270-6474/98/18239977-12$05.00/0
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