 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, December 15, 1998, 18(24):10310-10319
Use-Dependent Decline of Paired-Pulse Facilitation at Aplysia Sensory Neuron Synapses Suggests a Distinct Vesicle Pool or Release Mechanism
Xue-Ying Jiang and Thomas W. Abrams Departments of Pharmacology and Anesthesiology, University of Maryland School of Medicine, Baltimore, Maryland 21201-1559
We have characterized paired-pulse facilitation at Aplysia sensory neuron-to-motoneuron synapses. This simple form of very short-term synaptic plasticity displayed an unusual feature: it decreased dramatically with repeated testing. Synaptic depression at these synapses and this use-dependent decrease in paired-pulse facilitation occurred independently of each other. Paired-pulse facilitation was inversely correlated with the size of the initial synaptic connection and was absent at stronger synapses. The use-dependent decrease in paired-pulse facilitation occurred at the same rate at large synapses as at small synapses, although the initial paired-pulse facilitation at large synapses was substantially smaller. Rates of synaptic depression were also independent of initial synaptic strength. Paired-pulse facilitation was blocked by presynaptic EGTA injection, but not by postsynaptic EGTA or BAPTA injection. These results indicate that presynaptic Ca2+ influx plays a critical role in paired-pulse facilitation. However, the persistence of the decrease in paired-pulse facilitation for longer than 15 min suggests that Ca2+ from the first paired action potential produces facilitation via a modulatory mechanism rather than by summating with Ca2+ influx during the second paired action potential in activating the Ca2+ binding sites that initiate exocytosis. This modulatory mechanism may not involve protein phosphorylation because paired-pulse facilitation was unaffected by the protein kinase inhibitors H7 and KN-62. These findings further suggest that release by the second paired action potential occurs at sites distinct from those that mediate release by the first action potential.
Key words: synaptic plasticity; facilitation; synaptic depression; Aplysia; paired-pulse facilitation; calcium; phosphorylation
Copyright © 1998 Society for Neuroscience 0270-6474/98/182410310-10$05.00/0
This article has been cited by other articles:
A. Sakurai and P. S. Katz
State-, Timing-, and Pattern-Dependent Neuromodulation of Synaptic Strength by a Serotonergic Interneuron
J. Neurosci., January 7, 2009; 29(1): 268 - 279.
[Abstract] [Full Text] [PDF]
E. G. Antzoulatos and J. H. Byrne
Long-Term Sensitization Training Produces Spike Narrowing in Aplysia Sensory Neurons
J. Neurosci., January 17, 2007; 27(3): 676 - 683.
[Abstract] [Full Text] [PDF]
A. R. B. Schiess, C. S. Scullin, and L. D. Partridge
Neurosteroid-induced enhancement of short-term facilitation involves a component downstream from presynaptic calcium in hippocampal slices
J. Physiol., November 1, 2006; 576(3): 833 - 847.
[Abstract] [Full Text] [PDF]
B. Dumitriu, J. E. Cohen, Q. Wan, A. M. Negroiu, and T. W. Abrams
Serotonin Receptor Antagonists Discriminate Between PKA- and PKC-Mediated Plasticity in Aplysia Sensory Neurons
J Neurophysiol, April 1, 2006; 95(4): 2713 - 2720.
[Abstract] [Full Text] [PDF]
R. Menzel and G. Manz
Neural plasticity of mushroom body-extrinsic neurons in the honeybee brain
J. Exp. Biol., November 15, 2005; 208(22): 4317 - 4332.
[Abstract] [Full Text] [PDF]
G. A. Phares and J. H. Byrne
Analysis of 5-HT-Induced Short-Term Facilitation at Aplysia Sensorimotor Synapse During Bursts: Increased Synaptic Gain That Does Not Require ERK Activation
J Neurophysiol, July 1, 2005; 94(1): 871 - 877.
[Abstract] [Full Text] [PDF]
Y. Zhao and M. Klein
Changes in the Readily Releasable Pool of Transmitter and in Efficacy of Release Induced by High-Frequency Firing at Aplysia Sensorimotor Synapses in Culture
J Neurophysiol, April 1, 2004; 91(4): 1500 - 1509.
[Abstract] [Full Text] [PDF]
G. A. Phares, E. G. Antzoulatos, D. A. Baxter, and J. H. Byrne
Burst-Induced Synaptic Depression and Its Modulation Contribute to Information Transfer at Aplysia Sensorimotor Synapses: Empirical and Computational Analyses
J. Neurosci., September 10, 2003; 23(23): 8392 - 8401.
[Abstract] [Full Text] [PDF]
E. G. Antzoulatos, L. J. Cleary, A. Eskin, D. A. Baxter, and J. H. Byrne
Desensitization of Postsynaptic Glutamate Receptors Contributes to High-Frequency Homosynaptic Depression of Aplysia Sensorimotor Connections
Learn. Mem., September 1, 2003; 10(5): 309 - 313.
[Abstract] [Full Text] [PDF]
J. E. Cohen, C. U. Onyike, V. L. McElroy, A. H. Lin, and T. W. Abrams
Pharmacological Characterization of an Adenylyl Cyclase-Coupled 5-HT Receptor in Aplysia: Comparison With Mammalian 5-HT Receptors
J Neurophysiol, March 1, 2003; 89(3): 1440 - 1455.
[Abstract] [Full Text] [PDF]
A. I. Ivanov and R. L. Calabrese
Modulation of Spike-Mediated Synaptic Transmission by Presynaptic Background Ca2+ in Leech Heart Interneurons
J. Neurosci., February 15, 2003; 23(4): 1206 - 1218.
[Abstract] [Full Text] [PDF]
T. D. Gover, X.-Y. Jiang, and T. W. Abrams
Persistent, Exocytosis-Independent Silencing of Release Sites Underlies Homosynaptic Depression at Sensory Synapses in Aplysia
J. Neurosci., March 1, 2002; 22(5): 1942 - 1955.
[Abstract] [Full Text] [PDF]
A. R. Carter, C. Chen, P. M. Schwartz, and R. A. Segal
Brain-Derived Neurotrophic Factor Modulates Cerebellar Plasticity and Synaptic Ultrastructure
J. Neurosci., February 15, 2002; 22(4): 1316 - 1327.
[Abstract] [Full Text] [PDF]
J. H. Schaffhausen, T. M. Fischer, and T. J. Carew
Contirbution of Postsynaptic Ca2+ to the Induction of Posttetanic Potentiation in the Neural Circuit for Siphon Withdrawal in Aplysia
J. Neurosci., March 1, 2001; 21(5): 1739 - 1749.
[Abstract] [Full Text] [PDF]
S. Oleskevich and B. Walmsley
Phosphorylation regulates spontaneous and evoked transmitter release at a giant terminal in the rat auditory brainstem
J. Physiol., July 15, 2000; 526(2): 349 - 357.
[Abstract] [Full Text] [PDF]
N. T. Sherwood and D. C. Lo
Long-Term Enhancement of Central Synaptic Transmission by Chronic Brain-Derived Neurotrophic Factor Treatment
J. Neurosci., August 15, 1999; 19(16): 7025 - 7036.
[Abstract] [Full Text] [PDF]
J. A. D. Sanchez and M. D. Kirk
Short-Term Synaptic Enhancement Modulates Ingestion Motor Programs of Aplysia
J. Neurosci., July 15, 2000; 20(14): RC85 - RC85.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|