 |
 Previous Article | Next Article 
The Journal of Neuroscience, December 15, 1998, 18(24):10335-10344
Mammalian Nicotinic Receptors with  7 Subunits That Slowly
Desensitize and Rapidly Recover from -Bungarotoxin Blockade
Javier
Cuevas and
Darwin K.
Berg
Department of Biology, University of California, San Diego, La
Jolla, California 92093-0357
One of the most abundant nicotinic receptors in the nervous system
is a species that contains the  7 gene product, rapidly desensitizes,
and binds -bungarotoxin with great affinity. The receptor has a high
relative permeability to calcium and performs a variety of functions
including presynaptic modulation of transmitter release and
postsynaptic generation of synaptic currents. Fast excitatory
transmission in mammalian intracardiac ganglia is mediated primarily by
nicotinic receptors, and although intracardiac ganglion neurons express
the 7 gene, no toxin-sensitive response has been detected previously
in them. We report here that whole-cell patch-clamp recordings from
freshly dissociated intracardiac ganglion neurons reveal a nicotinic
response that desensitizes slowly and is blocked by -bungarotoxin in
a rapidly reversible manner. The only rat gene previously thought
capable of forming such receptors was 9, but no evidence suggests
that the 9 gene is expressed in neurons. We find that reverse
transcription (RT)-PCR detects 7 but not 9 mRNA in the
ganglia. In addition, the pharmacology of the nicotinic response is
typical of 7-containing receptors but differs in several respects
from that expected for 9. Binding experiments with immunotethered
receptors identifies a ganglionic species that contains the 7 gene
product. Moreover, intracellular perfusion of the cells with an
anti-7 monoclonal antibody specifically reduces the amplitude of the
toxin-sensitive response. The results indicate that 7-containing
receptors are responsible for the slowly desensitizing,
toxin-reversible response and suggest that the receptors are modified
in cell-specific ways to influence their functional properties.
Key words:
nicotinic; receptors; acetylcholine; intracardiac
ganglion; neuronal; 7; -bungarotoxin; patch clamp
Copyright © 1998 Society for Neuroscience 0270-6474/98/182410335-10$05.00/0
This article has been cited by other articles:
|
|
|
|
 
Y. Herrera, C. Katnik, J. D. Rodriguez, A. A. Hall, A. Willing, K. R. Pennypacker, and J. Cuevas
{sigma}-1 Receptor Modulation of Acid-Sensing Ion Channel a (ASIC1a) and ASIC1a-Induced Ca2+ Influx in Rat Cortical Neurons
J. Pharmacol. Exp. Ther.,
November 1, 2008;
327(2):
491 - 502.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Sharma, M. Grybko, and S. Vijayaraghavan
Action Potential-Independent and Nicotinic Receptor-Mediated Concerted Release of Multiple Quanta at Hippocampal CA3-Mossy Fiber Synapses
J. Neurosci.,
March 5, 2008;
28(10):
2563 - 2575.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. V. Mathew, A. J. Law, B. K. Lipska, M. I. Davila-Garcia, E. D. Zamora, S. N. Mitkus, R. Vakkalanka, R. E. Straub, D. R. Weinberger, J. E. Kleinman, et al.
{alpha}7 nicotinic acetylcholine receptor mRNA expression and binding in postmortem human brain are associated with genetic variation in neuregulin 1
Hum. Mol. Genet.,
December 1, 2007;
16(23):
2921 - 2932.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Katnik, W. R. Guerrero, K. R. Pennypacker, Y. Herrera, and J. Cuevas
Sigma-1 Receptor Activation Prevents Intracellular Calcium Dysregulation in Cortical Neurons during in Vitro Ischemia
J. Pharmacol. Exp. Ther.,
December 1, 2006;
319(3):
1355 - 1365.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-Z. Guo, Y. Liu, E. M. Sorenson, and V. A. Chiappinelli
Synaptically Released and Exogenous ACh Activates Different Nicotinic Receptors to Enhance Evoked Glutamatergic Transmission in the Lateral Geniculate Nucleus
J Neurophysiol,
October 1, 2005;
94(4):
2549 - 2560.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Rassadi, A. Krishnaswamy, B. Pie, R. McConnell, M. H. Jacob, and E. Cooper
A Null Mutation for the {alpha}3 Nicotinic Acetylcholine (ACh) Receptor Gene Abolishes Fast Synaptic Activity in Sympathetic Ganglia and Reveals That ACh Output from Developing Preganglionic Terminals Is Regulated in an Activity-Dependent Retrograde Manner
J. Neurosci.,
September 14, 2005;
25(37):
8555 - 8566.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Fayuk and J. L Yakel
Ca2+ permeability of nicotinic acetylcholine receptors in rat hippocampal CA1 interneurones
J. Physiol.,
August 1, 2005;
566(3):
759 - 768.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Deck, S. Bibevski, T. Gnecchi-Ruscone, V. Bellina, N. Montano, and M. E. Dunlap
{alpha}7-Nicotinic acetylcholine receptor subunit is not required for parasympathetic control of the heart in the mouse
Physiol Genomics,
June 16, 2005;
22(1):
86 - 92.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. I. DeHaven and J. Cuevas
VPAC Receptor Modulation of Neuroexcitability in Intracardiac Neurons: DEPENDENCE ON INTRACELLULAR CALCIUM MOBILIZATION AND SYNERGISTIC ENHANCEMENT BY PAC1 RECEPTOR ACTIVATION
J. Biol. Chem.,
September 24, 2004;
279(39):
40609 - 40621.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. G. Severance, H. Zhang, Y. Cruz, S. Pakhlevaniants, S. H. Hadley, J. Amin, L. Wecker, C. Reed, and J. Cuevas
The {alpha}7 Nicotinic Acetylcholine Receptor Subunit Exists in Two Isoforms that Contribute to Functional Ligand-Gated Ion Channels
Mol. Pharmacol.,
September 1, 2004;
66(3):
420 - 429.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Beker, M. Weber, R. H. A. Fink, and D. J. Adams
Muscarinic and Nicotinic ACh Receptor Activation Differentially Mobilize Ca2+ in Rat Intracardiac Ganglion Neurons
J Neurophysiol,
September 1, 2003;
90(3):
1956 - 1964.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Zhao, Y.-P. Kuo, A. A. George, J.-H. Peng, M. S. Purandare, K. M. Schroeder, R. J. Lukas, and J. Wu
Functional Properties of Homomeric, Human {alpha}7-Nicotinic Acetylcholine Receptors Heterologously Expressed in the SH-EP1 Human Epithelial Cell Line
J. Pharmacol. Exp. Ther.,
June 1, 2003;
305(3):
1132 - 1141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Salas, A. Orr-Urtreger, R. S. Broide, A. Beaudet, R. Paylor, and M. De Biasi
The Nicotinic Acetylcholine Receptor Subunit alpha 5 Mediates Short-Term Effects of Nicotine in Vivo
Mol. Pharmacol.,
May 1, 2003;
63(5):
1059 - 1066.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. G. Conroy, Q.-S. Liu, Q. Nai, J. F. Margiotta, and D. K. Berg
Potentiation of alpha 7-Containing Nicotinic Acetylcholine Receptors by Select Albumins
Mol. Pharmacol.,
February 1, 2003;
63(2):
419 - 428.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Sahibzada, M. Ferreira Jr, B. Williams, A. Wasserman, S. Vicini, and R. A Gillis
Nicotinic ACh receptor subtypes on gastrointestinally projecting neurones in the dorsal motor vagal nucleus of the rat
J. Physiol.,
December 15, 2002;
545(3):
1007 - 1016.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ji, T. Tosaka, B. H. Whitfield, A. N. Katchman, A. Kandil, B. C. Knollmann, and S. N. Ebert
Differential Rate Responses to Nicotine in Rat Heart: Evidence for Two Classes of Nicotinic Receptors
J. Pharmacol. Exp. Ther.,
June 1, 2002;
301(3):
893 - 899.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. S Khiroug, P. C Harkness, P. W Lamb, S. N Sudweeks, L. Khiroug, N. S Millar, and J. L Yakel
Rat nicotinic ACh receptor {alpha}7 and {beta}2 subunits co-assemble to form functional heteromeric nicotinic receptor channels
J. Physiol.,
April 15, 2002;
540(2):
425 - 434.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kawa
Acute synaptic modulation by nicotinic agonists in developing cerebellar Purkinje cells of the rat
J. Physiol.,
January 1, 2002;
538(1):
87 - 102.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Sgard, E. Charpantier, S. Bertrand, N. Walker, D. Caput, D. Graham, D. Bertrand, and F. Besnard
A Novel Human Nicotinic Receptor Subunit, alpha 10, That Confers Functionality to the alpha 9-Subunit
Mol. Pharmacol.,
January 1, 2002;
61(1):
150 - 159.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Fujino and D. Oertel
Cholinergic Modulation of Stellate Cells in the Mammalian Ventral Cochlear Nucleus
J. Neurosci.,
September 15, 2001;
21(18):
7372 - 7383.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ferreira, S. N. Ebert, D. C. Perry, R. P. Yasuda, C. M. Baker, M. I. Dávila-García, K. J. Kellar, and R. A. Gillis
Evidence of a Functional alpha 7-Neuronal Nicotinic Receptor Subtype Located on Motoneurons of the Dorsal Motor Nucleus of the Vagus
J. Pharmacol. Exp. Ther.,
April 13, 2001;
296(2):
260 - 269.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Q.-s. Liu, H. Kawai, and D. K. Berg
beta -Amyloid peptide blocks the response of alpha 7-containing nicotinic receptors on hippocampal neurons
PNAS,
March 22, 2001;
(2001)
81553598.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
V. T. Nguyen, A. Ndoye, and S. A. Grando
Novel Human {alpha}9 Acetylcholine Receptor Regulating Keratinocyte Adhesion is Targeted by Pemphigus Vulgaris Autoimmunity
Am. J. Pathol.,
October 1, 2000;
157(4):
1377 - 1391.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Bibevski, Y. Zhou, J. M. McIntosh, R. E. Zigmond, and M. E. Dunlap
Functional Nicotinic Acetylcholine Receptors That Mediate Ganglionic Transmission in Cardiac Parasympathetic Neurons
J. Neurosci.,
July 1, 2000;
20(13):
5076 - 5082.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cuevas, A. L Roth, and D. K Berg
Two distinct classes of functional {alpha}7-containing nicotinic receptor on rat superior cervical ganglion neurons
J. Physiol.,
June 15, 2000;
525(3):
735 - 746.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. C. Hogg, L. P. Miranda, D. J. Craik, R. J. Lewis, P. F. Alewood, and D. J. Adams
Single Amino Acid Substitutions in alpha -Conotoxin PnIA Shift Selectivity for Subtypes of the Mammalian Neuronal Nicotinic Acetylcholine Receptor
J. Biol. Chem.,
December 17, 1999;
274(51):
36559 - 36564.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q.-S. Liu and D. K. Berg
Extracellular Calcium Regulates Responses of Both alpha 3- and alpha 7-Containing Nicotinic Receptors on Chick Ciliary Ganglion Neurons
J Neurophysiol,
September 1, 1999;
82(3):
1124 - 1132.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q.-s. Liu, H. Kawai, and D. K. Berg
beta -Amyloid peptide blocks the response of alpha 7-containing nicotinic receptors on hippocampal neurons
PNAS,
April 10, 2001;
98(8):
4734 - 4739.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kawa
Acute synaptic modulation by nicotinic agonists in developing cerebellar purkinje cells of the rat
J. Physiol.,
December 3, 2001;
(2001)
200101288.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. S. Khiroug, P. C. Harkness, P. W. Lamb, S. N. Sudweeks, L. Khiroug, N. S. Millar, and J. L. Yakel
Rat nicotinic ACh receptor {alpha}7 and {beta}2 subunits co-assemble to form functional heteromeric nicotinic receptor channels
J. Physiol.,
February 22, 2002;
(2002)
200101384.
[Abstract]
[PDF]
|
|
|
|
