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The Journal of Neuroscience, February 1, 1998, 18(3):948-955
Patch-Clamp Recordings from Cerebellar Basket Cell Bodies and
Their Presynaptic Terminals Reveal an Asymmetric Distribution of
Voltage-Gated Potassium Channels
Andrew P.
Southan and
Brian
Robertson
Electrophysiology Group, Department of Biochemistry, Imperial
College of Science, Technology and Medicine, London SW7 2AY, United
Kingdom
Cerebellar basket cells form highly specialized inhibitory synaptic
contacts with Purkinje cells, namely the pericellular basket and
pinceau nerve terminal structures, wrapping around the Purkinje cell
somatic and axon hillock regions. These inhibitory synaptic contacts
are ideally located to control the ultimate output of the cerebellar
cortex. Previous immunohistochemical studies have shown that these
synaptic structures possess a very high density of the dendrotoxin
(DTX)-sensitive potassium channel subunit, Kv1.2. We have taken
advantage of this unique anatomical arrangement offering a high
concentration of identified Kv channel subunits by combining whole-cell
patch-clamp recording and fluorescence microscopy to establish a novel
preparation and perform the first recordings from unambiguously
identified mammalian CNS inhibitory presynaptic terminals. We report
that DTX-sensitive potassium channels are present in basket cell
terminals but not in the basket cell soma. This selective cellular
distribution suggests that these channels play an important role in
modulating cerebellar inhibitory synaptic transmission.
Key words:
potassium channels; cerebellum; synapse; whole cell; electrophysiology; mouse
Copyright © 1998 Society for Neuroscience 0270-6474/98/183948-08$05.00/0
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