 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, February 1, 1998, 18(3):965-974
Presynaptic Localization of Kv1.4-Containing A-Type Potassium Channels Near Excitatory Synapses in the Hippocampus
Edward C. Cooper1, Antonia Milroy2, Yuh Nung Jan3, Lily Yeh Jan3, and Daniel H. Lowenstein1, 2 Departments of 1 Neurology, 2 Anatomy, and 3 Physiology and Biochemistry and Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California 94143
Mammalian Shaker voltage-gated potassium channels that contain the Kv1.4 subunit exhibit rapid activation and prominent inactivation processes, which enable these channels to integrate brief (approximiately milliseconds) depolarizations over time intervals of up to tens of seconds. In the hippocampus, Kv1.4 immunoreactivity is detected at greatest density in two regions: (1) the middle molecular layer (MML), where perforant path axons synapse with dentate granule cells, and (2) the stratum lucidum (SL) of CA3, where the mossy fibers travel in tight fasciculi and form en passante synapses onto CA3 pyramidal cells. We have studied the localization of Kv1.4 within these regions in detail. First, we compared the distribution of Kv1.4 and synaptophysin (a synaptic vesicle protein primarily localized near termini) under confocal immunofluorescence microscopy. In the MML, Kv1.4 and synaptophysin immunofluorescence appeared to overlap. In the SL, however, Kv1.4 and synaptophysin staining was detected in nonoverlapping, irregular patches (~5-10 µm in diameter). Ultrastructural studies of these two regions revealed that Kv1.4 immunoreactivity was absent from the surface membranes of cell bodies and dendrites and occurred prominently on axons, including axonal "necks" near termini. Small excitatory synaptic boutons also were labeled in the MML; by contrast, the mossy fiber synaptic expansions in the SL were not stained. These localizations may enable Kv1.4-containing channels to regulate the process of neurotransmitter release at these excitatory synapses.
Key words: voltage-gated potassium channel; mossy fiber; presynaptic facilitation; Shaker; hippocampus; granule cells
Copyright © 1998 Society for Neuroscience 0270-6474/98/183965-10$05.00/0
This article has been cited by other articles:
J.-H. Lee, B.-H. Lee, S.-H. Choi, I.-S. Yoon, M. K. Pyo, T.-J. Shin, W.-S. Choi, Y. Lim, H. Rhim, K. H. Won, et al.
Ginsenoside Rg3 Inhibits Human Kv1.4 Channel Currents by Interacting with the Lys531 Residue
Mol. Pharmacol., March 1, 2008; 73(3): 619 - 626.
[Abstract] [Full Text] [PDF]
A. C. Jackson and B. P. Bean
State-Dependent Enhancement of Subthreshold A-Type Potassium Current by 4-Aminopyridine in Tuberomammillary Nucleus Neurons
J. Neurosci., October 3, 2007; 27(40): 10785 - 10796.
[Abstract] [Full Text] [PDF]
Q. Chen and H.-L. Pan
Signaling Mechanisms of Angiotensin II-Induced Attenuation of GABAergic Input to Hypothalamic Presympathetic Neurons
J Neurophysiol, May 1, 2007; 97(5): 3279 - 3287.
[Abstract] [Full Text] [PDF]
K. Vervaeke, N. Gu, C. Agdestein, H. Hu, and J. F. Storm
Kv7/KCNQ/M-channels in rat glutamatergic hippocampal axons and their role in regulation of excitability and transmitter release
J. Physiol., October 1, 2006; 576(1): 235 - 256.
[Abstract] [Full Text] [PDF]
D. Guan, J. C. F. Lee, T. Tkatch, D. J. Surmeier, W. E. Armstrong, and R. C. Foehring
Expression and biophysical properties of Kv1 channels in supragranular neocortical pyramidal neurones
J. Physiol., March 1, 2006; 571(2): 371 - 389.
[Abstract] [Full Text] [PDF]
K. Ono, T. Toyono, E. Honda, and K. Inenaga
Transient outward K+ currents in rat dissociated subfornical organ neurones and angiotensin II effects
J. Physiol., November 1, 2005; 568(3): 979 - 991.
[Abstract] [Full Text] [PDF]
A. Vydyanathan, Z.-Z. Wu, S.-R. Chen, and H.-L. Pan
A-Type Voltage-Gated K+ Currents Influence Firing Properties of Isolectin B4-Positive But Not Isolectin B4-Negative Primary Sensory Neurons
J Neurophysiol, June 1, 2005; 93(6): 3401 - 3409.
[Abstract] [Full Text] [PDF]
W. Wong and L. C. Schlichter
Differential Recruitment of Kv1.4 and Kv4.2 to Lipid Rafts by PSD-95
J. Biol. Chem., January 2, 2004; 279(1): 444 - 452.
[Abstract] [Full Text] [PDF]
R. Wissmann, W. Bildl, D. Oliver, M. Beyermann, H.-R. Kalbitzer, D. Bentrop, and B. Fakler
Solution Structure and Function of the "Tandem Inactivation Domain" of the Neuronal A-type Potassium Channel Kv1.4
J. Biol. Chem., April 25, 2003; 278(18): 16142 - 16150.
[Abstract] [Full Text] [PDF]
L. Liu and S. A. Simon
Modulation of IA Currents by Capsaicin in Rat Trigeminal Ganglion Neurons
J Neurophysiol, March 1, 2003; 89(3): 1387 - 1401.
[Abstract] [Full Text] [PDF]
W. Wong, E. W. Newell, D. G. M. Jugloff, O. T. Jones, and L. C. Schlichter
Cell Surface Targeting and Clustering Interactions between Heterologously Expressed PSD-95 and the Shal Voltage-gated Potassium Channel, Kv4.2
J. Biol. Chem., May 31, 2002; 277(23): 20423 - 20430.
[Abstract] [Full Text] [PDF]
H. Hu, L.-R. Shao, S. Chavoshy, N. Gu, M. Trieb, R. Behrens, P. Laake, O. Pongs, H. G. Knaus, O. P. Ottersen, et al.
Presynaptic Ca2+-Activated K+ Channels in Glutamatergic Hippocampal Terminals and Their Role in Spike Repolarization and Regulation of Transmitter Release
J. Neurosci., December 15, 2001; 21(24): 9585 - 9597.
[Abstract] [Full Text] [PDF]
M. N. Rasband, E. W. Park, T. W. Vanderah, J. Lai, F. Porreca, and J. S. Trimmer
Distinct potassium channels on pain-sensing neurons
PNAS, November 6, 2001; 98(23): 13373 - 13378.
[Abstract] [Full Text] [PDF]
P. A. Castro, E. C. Cooper, D. H. Lowenstein, and S. C. Baraban
Hippocampal Heterotopia Lack Functional Kv4.2 Potassium Channels in the Methylazoxymethanol Model of Cortical Malformations and Epilepsy
J. Neurosci., September 1, 2001; 21(17): 6626 - 6634.
[Abstract] [Full Text] [PDF]
M. M. Monaghan, J. S. Trimmer, and K. J. Rhodes
Experimental Localization of Kv1 Family Voltage-Gated K+ Channel {alpha} and {beta} Subunits in Rat Hippocampal Formation
J. Neurosci., August 15, 2001; 21(16): 5973 - 5983.
[Abstract] [Full Text] [PDF]
M. N. Nitabach, D. A. Llamas, R. C. Araneda, J. L. Intile, I. J. Thompson, Y. I. Zhou, and T. C. Holmes
A mechanism for combinatorial regulation of electrical activity: Potassium channel subunits capable of functioning as Src homology 3-dependent adaptors
PNAS, January 5, 2001; (2001) 31446198.
[Abstract] [Full Text]
D. J. Baro, A. Ayali, L. French, N. L. Scholz, J. Labenia, C. C. Lanning, K. Graubard, and R. M. Harris-Warrick
Molecular Underpinnings of Motor Pattern Generation: Differential Targeting of Shal and Shaker in the Pyloric Motor System
J. Neurosci., September 1, 2000; 20(17): 6619 - 6630.
[Abstract] [Full Text] [PDF]
G. Grosse, A. Draguhn, L. Hohne, R. Tapp, R. W. Veh, and G. Ahnert-Hilger
Expression of Kv1 Potassium Channels in Mouse Hippocampal Primary Cultures: Development and Activity-Dependent Regulation
J. Neurosci., March 1, 2000; 20(5): 1869 - 1882.
[Abstract] [Full Text] [PDF]
T. Tkatch, G. Baranauskas, and D. J. Surmeier
Kv4.2 mRNA Abundance and A-Type K+ Current Amplitude Are Linearly Related in Basal Ganglia and Basal Forebrain Neurons
J. Neurosci., January 15, 2000; 20(2): 579 - 588.
[Abstract] [Full Text] [PDF]
D. G. M. Jugloff, R. Khanna, L. C. Schlichter, and O. T. Jones
Internalization of the Kv1.4 Potassium Channel Is Suppressed by Clustering Interactions with PSD-95
J. Biol. Chem., January 14, 2000; 275(2): 1357 - 1364.
[Abstract] [Full Text] [PDF]
A. M. Tiffany, L. N. Manganas, E. Kim, Y.-P. Hsueh, M. Sheng, and J. S. Trimmer
PSD-95 and SAP97 Exhibit Distinct Mechanisms for Regulating K+ Channel Surface Expression and Clustering
J. Cell Biol., January 10, 2000; 148(1): 147 - 158.
[Abstract] [Full Text] [PDF]
N. Meiri, M.-K. Sun, Z. Segal, and D. L. Alkon
Memory and long-term potentiation (LTP) dissociated: Normal spatial memory despite CA1 LTP elimination with Kv1.4 antisense
PNAS, December 8, 1998; 95(25): 15037 - 15042.
[Abstract] [Full Text] [PDF]
I. L. Kopysova and D. Debanne
Critical Role of Axonal A-Type K+ Channels and Axonal Geometry in the Gating of Action Potential Propagation along CA3 Pyramidal Cell Axons: A Simulation Study
J. Neurosci., September 15, 1998; 18(18): 7436 - 7451.
[Abstract] [Full Text] [PDF]
M. N. Nitabach, D. A. Llamas, R. C. Araneda, J. L. Intile, I. J. Thompson, Y. I. Zhou, and T. C. Holmes
A mechanism for combinatorial regulation of electrical activity: Potassium channel subunits capable of functioning as Src homology 3-dependent adaptors
PNAS, January 16, 2001; 98(2): 705 - 710.
[Abstract] [Full Text] [PDF]
E. C. Cooper, K. D. Aldape, A. Abosch, N. M. Barbaro, M. S. Berger, W. S. Peacock, Y. N. Jan, and L. Y. Jan
Colocalization and coassembly of two human brain M-type potassium channel subunits that are mutated in epilepsy
PNAS, April 25, 2000; 97(9): 4914 - 4919.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|