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The Journal of Neuroscience, February 15, 1998, 18(4):1217-1229
Heterogeneity in the Molecular Composition of Excitatory Postsynaptic Sites during Development of Hippocampal Neurons in Culture
Anuradha Rao1, Eunjoon Kim2, Morgan Sheng2 and Ann Marie Craig1 1 Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, and 2 Howard Hughes Medical Institute, Massachusetts General Hospital, Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02114
To determine their roles in the assembly of glutamatergic postsynaptic sites, we studied the distributions of NMDA- and AMPA-type glutamate receptors; the NMDA receptor-interacting proteins
-actinin-2, PSD-95, and chapsyn; and the PSD-95-associated protein GKAP during the development of hippocampal neurons in culture. NMDA receptors first formed nonsynaptic proximal dendrite shaft clusters within 2-5 d. AMPA receptors were diffuse at this stage and began to cluster on spines at 9-10 d. NMDA receptor clusters remained partially nonsynaptic and mainly distinct from AMPA receptor clusters until after 3 weeks in culture, when the two began to colocalize at spiny synaptic sites. Thus, the localization of NMDA and AMPA receptors must be regulated by different mechanisms.
Key words: NMDA receptor; AMPA receptor; PSD-95; PSD-93; PDZ domain;
Copyright © 1998 Society for Neuroscience 0270-6474/98/1841217-13$05.00/0
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