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The Journal of Neuroscience, March 15, 1998, 18(6):2129-2146

Cell- and Lamina-Specific Expression and Activity-Dependent Regulation of Type II Calcium/Calmodulin-Dependent Protein Kinase Isoforms in Monkey Visual Cortex

Brahim Tighilet¹, Tsutomu Hashikawa², and Edward G. Jones¹

¹ Department of Anatomy and Neurobiology, University of California, Irvine, Irvine, California 92697-1280 and ² Laboratory of Brain Structure and Function, Frontier Research Program in Brain Mechanisms of Mind and Behavior, Institute of Physical and Chemical Research, Wako, Saitama 351-01, Japan

In situ hybridization histochemistry and immunocytochemistry were used to study localization and activity-dependent regulation of , , , and  isoforms of type II calcium/calmodulin-dependent protein kinase (CaMKII) and their mRNAs in areas 17 and 18 of normal and monocularly deprived adult macaques. CaMKII- is expressed overall at levels three to four times higher than that of CaMKII- and at least 15 times higher than that of CaMKII- and -. All isoforms are expressed primarily in pyramidal cells of both areas, especially those of layers II-III, IVA (in area 17), and VI, but are also expressed in nonpyramidal, non-GABAergic cells of layer IV of both areas and in interstitial neurons of the white matter. CaMKII- and - are colocalized, suggesting the formation of heteromers. There was no evidence of expression in neuroglial cells. Each isoform has a unique pattern of laminar and sublaminar distribution, but cortical layers or sublayers enriched for one isoform do not correlate with layers receiving inputs only from isoform-specific layers of the lateral geniculate nucleus. CaMKII- and - mRNA and protein levels in layer IVC of area 17 are subject to activity-dependent regulation, with brief periods of monocular deprivation caused by intraocular injections of tetrodotoxin leading to a 30% increase in CaMKII- mRNA and a comparable decrease in CaMKII- mRNA in deprived ocular dominance columns, especially of layer IVC. Expression in other layers and expression of CaMKII- and  were unaffected. Changes occurring in layer IVC may influence the formation of heteromers and protect supragranular layers from CaMKII-dependent plasticity in the adult.

Key words: plasticity; , , , and  isoforms; activity-dependent regulation; visual deprivation; pyramidal cells; GABA

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Copyright © 1998 Society for Neuroscience  0270-6474/98/1862129-18$05.00/0

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