The Journal of Neuroscience, March 15, 1998, 18(6):2129-2146
Cell- and Lamina-Specific Expression and Activity-Dependent
Regulation of Type II Calcium/Calmodulin-Dependent Protein Kinase
Isoforms in Monkey Visual Cortex
Brahim
Tighilet1,
Tsutomu
Hashikawa2, and
Edward G.
Jones1
1 Department of Anatomy and Neurobiology, University of
California, Irvine, Irvine, California 92697-1280 and
2 Laboratory of Brain Structure and Function, Frontier
Research Program in Brain Mechanisms of Mind and Behavior, Institute of
Physical and Chemical Research, Wako, Saitama 351-01, Japan
In situ hybridization histochemistry and
immunocytochemistry were used to study localization and
activity-dependent regulation of 
, 
, 
, and 
isoforms of
type II calcium/calmodulin-dependent protein kinase (CaMKII) and their
mRNAs in areas 17 and 18 of normal and monocularly deprived adult
macaques. CaMKII- is expressed overall at levels three to four times
higher than that of CaMKII- and at least 15 times higher than that
of CaMKII- and -. All isoforms are expressed primarily in
pyramidal cells of both areas, especially those of layers II-III, IVA
(in area 17), and VI, but are also expressed in nonpyramidal,
non-GABAergic cells of layer IV of both areas and in interstitial
neurons of the white matter. CaMKII- and - are colocalized,
suggesting the formation of heteromers. There was no evidence of
expression in neuroglial cells. Each isoform has a unique pattern of
laminar and sublaminar distribution, but cortical layers or sublayers
enriched for one isoform do not correlate with layers receiving inputs
only from isoform-specific layers of the lateral geniculate nucleus.
CaMKII- and - mRNA and protein levels in layer IVC of area 17 are
subject to activity-dependent regulation, with brief periods of
monocular deprivation caused by intraocular injections of tetrodotoxin
leading to a 30% increase in CaMKII- mRNA and a comparable decrease
in CaMKII- mRNA in deprived ocular dominance columns, especially of
layer IVC. Expression in other layers and expression of CaMKII-
and were unaffected. Changes occurring in layer IVC may influence
the formation of heteromers and protect supragranular layers from
CaMKII-dependent plasticity in the adult.
Key words:
plasticity; , , , and isoforms; activity-dependent regulation; visual deprivation; pyramidal cells; GABA
Copyright © 1998 Society for Neuroscience 0270-6474/98/1862129-18$05.00/0
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