QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (65) Citing Articles via Google Scholar Google Scholar Articles by Fueshko, S. M. Articles by Wray, S. Search for Related Content PubMed PubMed Citation Articles by Fueshko, S. M. Articles by Wray, S.

 Previous Article  |  Next Article 

The Journal of Neuroscience, April 1, 1998, 18(7):2560-2569

GABA Inhibits Migration of Luteinizing Hormone-Releasing Hormone Neurons in Embryonic Olfactory Explants

Susan M. Fueshko, Sharon Key, and Susan Wray

Laboratory of Neurochemistry, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20892-4130

During development, a subpopulation of olfactory neurons transiently expresses GABA. The spatiotemporal pattern of GABAergic expression coincides with migration of luteinizing hormone-releasing hormone (LHRH) neurons from the olfactory pit to the CNS. In this investigation, we evaluated the role of GABAergic input on LHRH neuronal migration using olfactory explants, previously shown to exhibit outgrowth of olfactory axons, migration of LHRH neurons in association with a subset of these axons, and the presence of the olfactory-derived GABAergic neuronal population. GABA_A receptor antagonists bicuculline (10⁵ M) or picrotoxin (10⁴ M) had no effect on the length of peripherin-immunoreactive olfactory fibers or LHRH cell number. However, LHRH cell migration, as determined by the distance immunopositive cells migrated from olfactory pits, was significantly increased by these perturbations. Addition of tetrodotoxin (10⁶ M), to inhibit Na⁺-transduced electrical activity, also significantly enhanced LHRH migration. The most robust effect observed was dramatic inhibition of LHRH cell migration in explants cultured in the presence of the GABA_A receptor agonist muscimol (10⁴ M). This study demonstrates that GABAergic activity in nasal regions can have profound effects on migration of LHRH neurons and suggests that GABA participates in appropriate timing of LHRH neuronal migration into the developing brain.

Key words: GABA; GnRH; olfactory; peripherin; tetrodotoxin; immunocytochemistry

---

Copyright © 1998 Society for Neuroscience  0270-6474/98/1872560-10$05.00/0

This article has been cited by other articles:

				A. Pierce, B. Bliesner, M. Xu, S. Nielsen-Preiss, G. Lemke, S. Tobet, and M. E. Wierman Axl and Tyro3 Modulate Female Reproduction by Influencing Gonadotropin-Releasing Hormone Neuron Survival and Migration Mol. Endocrinol., November 1, 2008; 22(11): 2481 - 2495. [Abstract] [Full Text] [PDF]

				J. C. Gill, B. Wadas, P. Chen, W. Portillo, A. Reyna, E. Jorgensen, S. Mani, G. A. Schwarting, S. M. Moenter, S. Tobet, et al. The Gonadotropin-Releasing Hormone (GnRH) Neuronal Population Is Normal in Size and Distribution in GnRH-Deficient and GnRH Receptor-Mutant Hypogonadal Mice Endocrinology, September 1, 2008; 149(9): 4596 - 4604. [Abstract] [Full Text] [PDF]

				P. Giacobini, A. Messina, S. Wray, C. Giampietro, T. Crepaldi, P. Carmeliet, and A. Fasolo Hepatocyte Growth Factor Acts as a Motogen and Guidance Signal for Gonadotropin Hormone-Releasing Hormone-1 Neuronal Migration J. Neurosci., January 10, 2007; 27(2): 431 - 445. [Abstract] [Full Text] [PDF]

				G. A. Schwarting, T. R. Henion, J. D. Nugent, B. Caplan, and S. Tobet Stromal cell-derived factor-1 (chemokine C-X-C motif ligand 12) and chemokine C-X-C motif receptor 4 are required for migration of gonadotropin-releasing hormone neurons to the forebrain. J. Neurosci., June 21, 2006; 26(25): 6834 - 6840. [Abstract] [Full Text] [PDF]

				S. A. Tobet and G. A. Schwarting Minireview: Recent Progress in Gonadotropin-Releasing Hormone Neuronal Migration Endocrinology, March 1, 2006; 147(3): 1159 - 1165. [Abstract] [Full Text] [PDF]

				W. J. Moody and M. M. Bosma Ion Channel Development, Spontaneous Activity, and Activity-Dependent Development in Nerve and Muscle Cells Physiol Rev, July 1, 2005; 85(3): 883 - 941. [Abstract] [Full Text] [PDF]

				J.-B. Manent, M. Demarque, I. Jorquera, C. Pellegrino, Y. Ben-Ari, L. Aniksztejn, and A. Represa A Noncanonical Release of GABA and Glutamate Modulates Neuronal Migration J. Neurosci., May 11, 2005; 25(19): 4755 - 4765. [Abstract] [Full Text] [PDF]

				E. P. Bless, H. J. Walker, K. W. Yu, J. G. Knoll, S. M. Moenter, G. A. Schwarting, and S. A. Tobet Live View of Gonadotropin-Releasing Hormone Containing Neuron Migration Endocrinology, January 1, 2005; 146(1): 463 - 468. [Abstract] [Full Text] [PDF]

				A. J. Bolteus and A. Bordey GABA Release and Uptake Regulate Neuronal Precursor Migration in the Postnatal Subventricular Zone J. Neurosci., September 1, 2004; 24(35): 7623 - 7631. [Abstract] [Full Text] [PDF]

				J. C. Gill, S. M. Moenter, and P.-S. Tsai Developmental Regulation of Gonadotropin-Releasing Hormone Neurons by Fibroblast Growth Factor Signaling Endocrinology, August 1, 2004; 145(8): 3830 - 3839. [Abstract] [Full Text] [PDF]

				F. Moya and M. Valdeolmillos Polarized Increase of Calcium and Nucleokinesis in Tangentially Migrating Neurons Cereb Cortex, June 1, 2004; 14(6): 610 - 618. [Abstract] [Full Text] [PDF]

				P. Giacobini, A. S. Kopin, P. M. Beart, L. D. Mercer, A. Fasolo, and S. Wray Cholecystokinin Modulates Migration of Gonadotropin-Releasing Hormone-1 Neurons J. Neurosci., May 19, 2004; 24(20): 4737 - 4748. [Abstract] [Full Text] [PDF]

				S. A. Tobet, H. J. Walker, M. L. Seney, and K. W. Yu Viewing Cell Movements in the Developing Neuroendocrine Brain Integr. Comp. Biol., December 1, 2003; 43(6): 794 - 801. [Abstract] [Full Text] [PDF]

				D D Wang, D D Krueger, and A Bordey GABA Depolarizes Neuronal Progenitors of the Postnatal Subventricular Zone Via GABAA Receptor Activation J. Physiol., August 1, 2003; 550(3): 785 - 800. [Abstract] [Full Text] [PDF]

				S. Heger, M. Seney, E. Bless, G. A. Schwarting, M. Bilger, A. Mungenast, S. R. Ojeda, and S. A. Tobet Overexpression of Glutamic Acid Decarboxylase-67 (GAD-67) in Gonadotropin-Releasing Hormone Neurons Disrupts Migratory Fate and Female Reproductive Function in Mice Endocrinology, June 1, 2003; 144(6): 2566 - 2579. [Abstract] [Full Text] [PDF]

				N. Sharifi, A. E. Reuss, and S. Wray Prenatal LHRH Neurons in Nasal Explant Cultures Express Estrogen Receptor {beta} Transcript Endocrinology, July 1, 2002; 143(7): 2503 - 2507. [Abstract] [Full Text] [PDF]

				S.-K. Han, I. M. Abraham, and A. E. Herbison Effect of GABA on GnRH Neurons Switches from Depolarization to Hyperpolarization at Puberty in the Female Mouse Endocrinology, April 1, 2002; 143(4): 1459 - 1466. [Abstract] [Full Text] [PDF]

				T. N. Behar, S. V. Smith, R. T. Kennedy, J. M. Mckenzie, I. Maric, and J. L. Barker GABAB Receptors Mediate Motility Signals for Migrating Embryonic Cortical Cells Cereb Cortex, August 1, 2001; 11(8): 744 - 753. [Abstract] [Full Text] [PDF]

				E. Terasawa and D. L. Fernandez Neurobiological Mechanisms of the Onset of Puberty in Primates Endocr. Rev., February 1, 2001; 22(1): 111 - 151. [Abstract] [Full Text]

				G. A. Schwarting, C. Kostek, E. P. Bless, N. Ahmad, and S. A. Tobet Deleted in Colorectal Cancer (DCC) Regulates the Migration of Luteinizing Hormone-Releasing Hormone Neurons to the Basal Forebrain J. Neurosci., February 1, 2001; 21(3): 911 - 919. [Abstract] [Full Text] [PDF]

				S. X. Simonian and A. E. Herbison Differing, Spatially Restricted Roles of Ionotropic Glutamate Receptors in Regulating the Migration of GnRH Neurons during Embryogenesis J. Neurosci., February 1, 2001; 21(3): 934 - 943. [Abstract] [Full Text] [PDF]

				P. R. Kramer, R. Krishnamurthy, P. J. Mitchell, and S. Wray Transcription Factor Activator Protein-2 Is Required for Continued Luteinizing Hormone-Releasing Hormone Expression in the Forebrain of Developing Mice Endocrinology, May 1, 2000; 141(5): 1823 - 1838. [Abstract] [Full Text] [PDF]

				E. P. Bless, W. A. Westaway, G. A. Schwarting, and S. A. Tobet Effects of {gamma}-Aminobutyric AcidA Receptor Manipulation on Migrating Gonadotropin-Releasing Hormone Neurons through the Entire Migratory Route in Vivo and in Vitro Endocrinology, March 1, 2000; 141(3): 1254 - 1262. [Abstract] [Full Text] [PDF]

				E. Terasawa, W. K. Schanhofer, K. L. Keen, and L. Luchansky Intracellular Ca2+ Oscillations in Luteinizing Hormone-Releasing Hormone Neurons Derived from the Embryonic Olfactory Placode of the Rhesus Monkey J. Neurosci., July 15, 1999; 19(14): 5898 - 5909. [Abstract] [Full Text] [PDF]

				A. C. Puche and M. T. Shipley Odor-Induced, Activity-Dependent Transneuronal Gene Induction In Vitro: Mediation by NMDA Receptors J. Neurosci., February 15, 1999; 19(4): 1359 - 1370. [Abstract] [Full Text] [PDF]

				S. M. Fueshko, S. Key, and S. Wray Luteinizing Hormone Releasing Hormone (LHRH) Neurons Maintained in Nasal Explants Decrease LHRH Messenger Ribonucleic Acid Levels after Activation of GABAA Receptors Endocrinology, June 1, 1998; 139(6): 2734 - 2740. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help