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The Journal of Neuroscience, April 15, 1998, 18(8):2954-2961
Evidence for a Tetrameric Structure of Recombinant NMDA Receptors
Bodo Laube, Jochen Kuhse, and Heinrich Betz Department of Neurochemistry, Max-Planck-Institute for Brain Research, 60528 Frankfurt/Main, Germany
The amino acids L-glutamate and glycine are essential agonists of the excitatory NMDA receptor, a subtype of the ionotropic glutamate receptor family. The native NMDA receptor is composed of two types of homologous membrane-spanning subunits, NR1 and NR2. Here, the numbers of glycine-binding NR1 and glutamate-binding NR2 subunits in the NMDA receptor hetero-oligomer were determined by coexpressing the wild-type (wt) NR1 with the low-affinity mutant NR1Q387K, and the wt NR2B with the low-affinity mutant NR2BE387A, subunits in Xenopus oocytes. In both cases, analysis of the resulting dose-response curves revealed three independent components of glycine and glutamate sensitivity. These correspond to the respective wild-type and mutant affinities and an additional intermediate hybrid affinity, indicating the existence of three discrete receptor populations. Binomial analysis of these data indicates the presence of two glycine and two glutamate binding subunits in the functional receptor. In addition, we analyzed the inhibitory effects of the negative dominant NR1R505K and NR2BR493K mutants on maximal inducible whole-cell currents of wt NR1/NR2B receptors. The inhibition profiles obtained on expression of increasing amounts of these mutant proteins again were fitted best by assuming an incorporation of two NR1 and two NR2 subunits into the receptor hetero-oligomer. Our data are consistent with NMDA receptors being tetrameric proteins that are composed of four homologous subunits.
Key words: mutagenesis; subunit stoichiometry; NMDA receptor; glutamate; channel assembly; electrophysiology; agonist affinity; Xenopus oocyte
Copyright © 1998 Society for Neuroscience 0270-6474/98/1882954-08$05.00/0
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