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The Journal of Neuroscience, May 1, 1998, 18(9):3138-3146

G-Protein-Coupled Modulation of Presynaptic Calcium Currents and Transmitter Release by a GABA_B Receptor

Tomoyuki Takahashi, Yoshinao Kajikawa, and Tetsuhiro Tsujimoto

Department of Neurophysiology, University of Tokyo Faculty of Medicine, Tokyo 113, Japan

Presynaptic GABA_B receptors play a regulatory role in central synaptic transmission. To elucidate their underlying mechanism of action, we have made whole-cell recordings of calcium and potassium currents from a giant presynaptic terminal, the calyx of Held, and EPSCs from its postsynaptic target in the medial nucleus of the trapezoid body of rat brainstem slices. The GABA_B receptor agonist baclofen suppressed EPSCs and presynaptic calcium currents but had no effect on voltage-dependent potassium currents. The calcium current-EPSC relationship measured during baclofen application was similar to that observed on reducing [Ca²⁺]_o, suggesting that the presynaptic inhibition generated by baclofen is caused largely by the suppression of presynaptic calcium influx. Presynaptic loading of the GDP analog guanosine-5'-O-(2-thiodiphosphate) (GDPS) abolished the effect of baclofen on both presynaptic calcium currents and EPSCs. The nonhydrolyzable GTP analog guanosine 5'-O-(3-thiotriphosphate) (GTPS) suppressed presynaptic calcium currents and occluded the effect of baclofen on presynaptic calcium currents and EPSCs. Photoactivation of GTPS induced an inward rectifying potassium current at the calyx of Held, whereas baclofen had no such effect. We conclude that presynaptic GABA_B receptors suppress transmitter release through G-protein-coupled inhibition of calcium currents.

Key words: GABA_B receptor; presynaptic inhibition; Gprotein; calcium currents; inwardly rectifying potassium currents; the calyx of Held; presynaptic recording

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Copyright © 1998 Society for Neuroscience  0270-6474/98/1893138-09$05.00/0

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