 |
 Previous Article | Next Article 
The Journal of Neuroscience, May 1, 1998, 18(9):3224-3232
High Constitutive NF- B Activity Mediates Resistance to
Oxidative Stress in Neuronal Cells
Frank
Lezoualc'h1,
Yutaka
Sagara2,
Florian
Holsboer1, and
Christian
Behl1
1 Max-Planck-Institute of Psychiatry, 80804 Munich,
Germany, and 2 The Salk Institute for Biological
Studies, San Diego, California 90370
Selected clones of the sympathetic precursor-like cell line PC12
(rCl8) are resistant to oxidative cell death induced by the Alzheimer's disease-associated amyloid  protein (A) and hydrogen peroxide (H2O2). Here, we show that the
transcriptional activity and DNA binding activity of the
redox-sensitive transcription factor NF- B and its nuclear expression
are constitutively increased in rCl8 cells compared with their
nonresistant parental PC12 cell (PC12p) counterpart. Suppression of the
transcriptional activity of NF-B in rCl8 cells with the synthetic
glucocorticoid dexamethasone or by direct overexpression of a
super-repressor mutant form of IB , a specific inhibitor of
NF-B, reversed the oxidative stress resistance phenotype of these
cells and ultimately led to increased cell death after the challenge
with H2O2. Dexamethasone treatment also caused
an increase in the protein level of IB. Our data show that an
increased baseline of NF-B activity may mediate the resistance of
these cells of neuronal origin to oxidative stress. Therefore, the
presented model may help to identify possible neuronal target genes of
NF-B and to further elucidate the molecular basis of the
differential sensitivity of neurons in neurodegenerative conditions
associated with an increased oxidative burden, such as in Alzheimer's
disease.
Key words:
NF-B; Alzheimer's disease; amyloid protein; oxidative stress; antioxidant enzymes; glucocorticoids; neuroprotection
Copyright © 1998 Society for Neuroscience 0270-6474/98/1893224-09$05.00/0
This article has been cited by other articles:
|
|
|
|
 
C. M. Alvira, A. Abate, G. Yang, P. A. Dennery, and M. Rabinovitch
Nuclear Factor-{kappa}B Activation in Neonatal Mouse Lung Protects against Lipopolysaccharide-induced Inflammation
Am. J. Respir. Crit. Care Med.,
April 15, 2007;
175(8):
805 - 815.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Goswami, P. Dikshit, A. Mishra, N. Nukina, and N. R. Jana
Expression of Expanded Polyglutamine Proteins Suppresses the Activation of Transcription Factor NF{kappa}B
J. Biol. Chem.,
December 1, 2006;
281(48):
37017 - 37024.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-H. Jang and Y.-J. Surh
Bcl-2 Attenuation of Oxidative Cell Death Is Associated with Up-regulation of {gamma}-Glutamylcysteine Ligase via Constitutive NF-{kappa}B Activation
J. Biol. Chem.,
September 10, 2004;
279(37):
38779 - 38786.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. De Bosscher, W. Vanden Berghe, and G. Haegeman
The Interplay between the Glucocorticoid Receptor and Nuclear Factor-{kappa}B or Activator Protein-1: Molecular Mechanisms for Gene Repression
Endocr. Rev.,
August 1, 2003;
24(4):
488 - 522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F. Sanchez, L. F. Sniderhan, A. L. Williamson, S. Fan, S. Chakraborty-Sett, and S. B. Maggirwar
Glycogen Synthase Kinase 3{beta}-Mediated Apoptosis of Primary Cortical Astrocytes Involves Inhibition of Nuclear Factor {kappa}B Signaling
Mol. Cell. Biol.,
July 1, 2003;
23(13):
4649 - 4662.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Marchalonis, G. K. Whitfield, and S. F. Schluter
Rapid Evolutionary Emergence of the Combinatorial Recognition Repertoire
Integr. Comp. Biol.,
April 1, 2003;
43(2):
347 - 359.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Lotharius, S. Barg, P. Wiekop, C. Lundberg, H. K. Raymon, and P. Brundin
Effect of Mutant alpha -Synuclein on Dopamine Homeostasis in a New Human Mesencephalic Cell Line
J. Biol. Chem.,
October 4, 2002;
277(41):
38884 - 38894.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. L. Bhakar, L.-L. Tannis, C. Zeindler, M. P. Russo, C. Jobin, D. S. Park, S. MacPherson, and P. A. Barker
Constitutive Nuclear Factor-kappa B Activity Is Required for Central Neuron Survival
J. Neurosci.,
October 1, 2002;
22(19):
8466 - 8475.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Pizzi, F. Goffi, F. Boroni, M. Benarese, S. E. Perkins, H.-C. Liou, and P. Spano
Opposing Roles for NF-kappa B/Rel Factors p65 and c-Rel in the Modulation of Neuron Survival Elicited by Glutamate and Interleukin-1beta
J. Biol. Chem.,
May 31, 2002;
277(23):
20717 - 20723.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yabe, D. Wilson, and J. P. Schwartz
NFkappa B Activation Is Required for the Neuroprotective Effects of Pigment Epithelium-derived Factor (PEDF) on Cerebellar Granule Neurons
J. Biol. Chem.,
November 9, 2001;
276(46):
43313 - 43319.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. A. Smits, A. J. van Beelen, N. M. de Vos, A. Rijsmus, T. van der Bruggen, J. Verhoef, F. L. van Muiswinkel, and H. S. L. M. Nottet
Activation of Human Macrophages by Amyloid-{{beta}} Is Attenuated by Astrocytes
J. Immunol.,
June 1, 2001;
166(11):
6869 - 6876.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Lucassen, M. B. Muller, F. Holsboer, J. Bauer, A. Holtrop, J. Wouda, W. J. G. Hoogendijk, E. R. De Kloet, and D. F. Swaab
Hippocampal Apoptosis in Major Depression Is a Minor Event and Absent from Subareas at Risk for Glucocorticoid Overexposure
Am. J. Pathol.,
February 1, 2001;
158(2):
453 - 468.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Wolozin and C. Behl
Mechanisms of Neurodegenerative Disorders: Part 2: Control of Cell Death
Arch Neurol,
June 1, 2000;
57(6):
801 - 804.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Stanciu, Y. Wang, R. Kentor, N. Burke, S. Watkins, G. Kress, I. Reynolds, E. Klann, M. R. Angiolieri, J. W. Johnson, et al.
Persistent Activation of ERK Contributes to Glutamate-induced Oxidative Toxicity in a Neuronal Cell Line and Primary Cortical Neuron Cultures
J. Biol. Chem.,
April 14, 2000;
275(16):
12200 - 12206.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Christen
Oxidative stress and Alzheimer disease1
Am. J. Clinical Nutrition,
February 1, 2000;
71(2):
621s - 629s.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Ernfors
Nuclear Factor-{kappa}B to the Rescue of Cytokine-induced Neuronal Survival
J. Cell Biol.,
January 24, 2000;
148(2):
223 - 226.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Kaltschmidt, M. Uherek, H. Wellmann, B. Volk, and C. Kaltschmidt
Inhibition of NF-kappa B potentiates amyloid beta -mediated neuronal apoptosis
PNAS,
August 3, 1999;
96(16):
9409 - 9414.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. G. Sullivan, A. J. Bruce-Keller, A. G. Rabchevsky, S. Christakos, D. K. St. Clair, M. P. Mattson, and S. W. Scheff
Exacerbation of Damage and Altered NF-kappa B Activation in Mice Lacking Tumor Necrosis Factor Receptors after Traumatic Brain Injury
J. Neurosci.,
August 1, 1999;
19(15):
6248 - 6256.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Luo, A. Hattori, J. Munoz, Z.-H. Qin, and G. S. Roth
Intrastriatal Dopamine Injection Induces Apoptosis Through Oxidation-Involved Activation of Transcription Factors AP-1 and NF-kappa B in Rats
Mol. Pharmacol.,
August 1, 1999;
56(2):
254 - 264.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Heck, F. Lezoualc'h, S. Engert, and C. Behl
Insulin-like Growth Factor-1-mediated Neuroprotection against Oxidative Stress Is Associated with Activation of Nuclear Factor kappa B
J. Biol. Chem.,
April 2, 1999;
274(14):
9828 - 9835.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Post, F. Holsboer, and C. Behl
Induction of NF-kappa B Activity during Haloperidol-Induced Oxidative Toxicity in Clonal Hippocampal Cells: Suppression of NF-kappa B and Neuroprotection by Antioxidants
J. Neurosci.,
October 15, 1998;
18(20):
8236 - 8246.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Wellmann, B. Kaltschmidt, and C. Kaltschmidt
Retrograde Transport of Transcription Factor NF-kappa B in Living Neurons
J. Biol. Chem.,
April 6, 2001;
276(15):
11821 - 11829.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
