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The Journal of Neuroscience, May 1, 1998, 18(9):3233-3240
Attenuated Influx of Calcium Ions at Nerve Endings of csp and shibire Mutant Drosophila
Joy A. Umbach1, Minoru Saitoe2, Yoshi Kidokoro2, and Cameron B. Gundersen1 1 The Department of Molecular and Medical Pharmacology and The Crump Institute for Biological Imaging, University of California Los Angeles School of Medicine, Los Angeles, California 90095, and 2 Institute for Behavioral Sciences, Gunma University School of Medicine, Maebashi 371, Japan
Previous work has shown that cysteine-string proteins (csps) are synaptic vesicle proteins that are important for evoked neurotransmitter release at Drosophila neuromuscular junctions. Indirect evidence has implicated csps in a regulatory link between synaptic vesicles and presynaptic calcium (Ca) channels. In this report, we use Ca Crimson to monitor stimulus-dependent changes of cytosolic Ca at motor nerve terminals of csp mutant Drosophila. These mutants display temperature-sensitive (TS) paralysis and a presynaptic failure of evoked synaptic transmission. We show that this TS inhibition of neuromuscular transmission is correlated with a block of Ca ion entry at nerve endings of csp mutants. These data support the hypothesis that csps mediate a regulatory interaction between synaptic vesicles and presynaptic Ca channels. Moreover, these results predict that if one depletes nerve endings of synaptic vesicles, one may see a reduction of presynaptic Ca ion entry. Defects of the dynamin gene in TS shibire mutant Drosophila interfere with synaptic vesicle recycling and lead to an activity-dependent depletion of these organelles. Our results show that Ca influx is blocked at nerve terminals of shibire mutant larvae at the same time that synaptic transmission fails in these organisms. Thus, using two completely independent Drosophila mutants, we demonstrate that synaptic vesicles and csps are vital for the function of presynaptic Ca channels.
Key words: Ca imaging; cysteine string proteins; Ca channels; presynaptic function; shibire; Drosophila
Copyright © 1998 Society for Neuroscience 0270-6474/98/1893233-08$05.00/0
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