 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, May 1, 1998, 18(9):3363-3372
Neurolin, the Goldfish Homolog of DM-GRASP, Is Involved in Retinal Axon Pathfinding to the Optic Disk
Heiko Ott, Martin Bastmeyer, and Claudia A. O. Stuermer Department of Biology, University of Konstanz, 78457 Konstanz, Germany
Young axons of new retinal ganglion cells (RGCs) in the continuously growing goldfish retina fasciculate with one another and their immediate forerunners on their path toward the optic disk and along the optic nerve. They express the immunoglobulin superfamily cell adhesion molecules (CAMs) neurolin (DM-GRASP) and the L1-like E587 antigen. Repeated injections of Fab fragments from polyclonal antisera against neurolin (neurolin Fabs) into the eye of 3.4-cm-long and rapidly growing goldfish caused highly aberrant pathways of young RGC axon subfascicles in the dorsal retina. Many axons grew in circles and failed to reach the optic disk. In contrast, E587 Fabs, used in parallel experiments, disrupted the fascicles but did not interfere with the disk-directed growth. Neurolin Fabs also disturbed axonal fasciculation in vivo as well as in vitro but less severely than E587 Fabs. Coinjections of both Fabs increased defasciculation of the dorsal axons in both aberrant and disk-directed routes. They also disrupted the order of young RGC axons in the optic nerve more severely than E587 Fabs alone. This demonstrates that the development of tight and orderly fascicles in the dorsal retina and in the optic nerve requires both E587 antigen and neurolin. More importantly, our results suggest an involvement of neurolin in RGC axonal guidance from the retinal periphery to the optic disk. Because disrupted fascicles and errant axon routes were found only in the dorsal retinal half, a cooperation with so-called positional markers may be conceived.
Key words: retinal ganglion cell axons; pathfinding errors; cell adhesion molecules; immunoglobulin superfamily; antibodies; intraocular injections
Copyright © 1998 Society for Neuroscience 0270-6474/98/1893363-10$05.00/0
This article has been cited by other articles:
A. J. Pittman, M.-Y. Law, and C.-B. Chien
Pathfinding in a large vertebrate axon tract: isotypic interactions guide retinotectal axons at multiple choice points
Development, September 1, 2008; 135(17): 2865 - 2871.
[Abstract] [Full Text] [PDF]
H. Thompson, O. Camand, D. Barker, and L. Erskine
Slit Proteins Regulate Distinct Aspects of Retinal Ganglion Cell Axon Guidance within Dorsal and Ventral Retina
J. Neurosci., August 2, 2006; 26(31): 8082 - 8091.
[Abstract] [Full Text] [PDF]
A. Kolpak, J. Zhang, and Z.-Z. Bao
Sonic Hedgehog Has a Dual Effect on the Growth of Retinal Ganglion Axons Depending on Its Concentration
J. Neurosci., March 30, 2005; 25(13): 3432 - 3441.
[Abstract] [Full Text] [PDF]
J. Zhang, Z. Jin, and Z.-Z. Bao
Disruption of gradient expression of Zic3 resulted in abnormal intra-retinal axon projection
Development, April 1, 2004; 131(7): 1553 - 1562.
[Abstract] [Full Text] [PDF]
S F Oster and D W Sretavan
Connecting the eye to the brain: the molecular basis of ganglion cell axon guidance
Br J Ophthalmol, May 1, 2003; 87(5): 639 - 645.
[Abstract] [Full Text] [PDF]
G. P. Demyanenko and P. F. Maness
The L1 Cell Adhesion Molecule Is Essential for Topographic Mapping of Retinal Axons
J. Neurosci., January 15, 2003; 23(2): 530 - 538.
[Abstract] [Full Text] [PDF]
C. Lillo, A. Velasco, D. Jimeno, E. Cid, J. M. Lara, and J. Aijon
The Glial Design of a Teleost Optic Nerve Head Supporting Continuous Growth
J. Histochem. Cytochem., October 1, 2002; 50(10): 1289 - 1302.
[Abstract] [Full Text] [PDF]
E. Birgbauer, S. F. Oster, C. G. Severin, and D. W. Sretavan
Retinal axon growth cones respond to EphB extracellular domains as inhibitory axon guidance cues
Development, August 1, 2001; 128(15): 3041 - 3048.
[Abstract] [Full Text] [PDF]
B. Petrausch, R. Tabibiazar, T. Roser, Y. Jing, D. Goldman, C. A. O. Stuermer, N. Irwin, and L. I. Benowitz
A Purine-Sensitive Pathway Regulates Multiple Genes Involved in Axon Regeneration in Goldfish Retinal Ganglion Cells
J. Neurosci., November 1, 2000; 20(21): 8031 - 8041.
[Abstract] [Full Text] [PDF]
E Birgbauer, C. Cowan, D. Sretavan, and M Henkemeyer
Kinase independent function of EphB receptors in retinal axon pathfinding to the optic disc from dorsal but not ventral retina
Development, January 3, 2000; 127(6): 1231 - 1241.
[Abstract] [PDF]
M. M. Ledig, F. Haj, J. L. Bixby, A. W. Stoker, and B. K. Mueller
The Receptor Tyrosine Phosphatase Cryp{alpha} Promotes Intraretinal Axon Growth
J. Cell Biol., October 18, 1999; 147(2): 375 - 388.
[Abstract] [Full Text] [PDF]
A. Marg, P. Sirim, F. Spaltmann, A. Plagge, G. Kauselmann, F. Buck, F. G. Rathjen, and T. Brummendorf
Neurotractin, A Novel Neurite Outgrowth-promoting Ig-like Protein that Interacts with CEPU-1 and LAMP
J. Cell Biol., May 17, 1999; 145(4): 865 - 876.
[Abstract] [Full Text] [PDF]
C. A. Leppert, H. Diekmann, C. Paul, U. Laessing, M. Marx, M. Bastmeyer, and C. A.O. Stuermer
Neurolin Ig Domain 2 Participates in Retinal Axon Guidance and Ig Domains 1 and 3 in Fasciculation
J. Cell Biol., January 25, 1999; 144(2): 339 - 349.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|