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The Journal of Neuroscience, May 1, 1998, 18(9):3480-3487
Phosphorylation of Mitogen-Activated Protein Kinase by One-Trial
and Multi-Trial Classical Conditioning
Terry
Crow1,
Juan-Juan
Xue-Bian1,
Vilma
Siddiqi1,
Yuan
Kang2, and
Joseph T.
Neary2, 3
1 Department of Neurobiology and Anatomy, University of
Texas Medical School at Houston, Houston, Texas 77225, and
2 Research Service, Veterans Affairs Medical Center and
3 Departments of Pathology, and Biochemistry and Molecular
Biology, University of Miami School of Medicine, Miami, Florida 33125
The pathway supporting the conditioned stimulus (CS) is one site of
plasticity that has been studied extensively in conditioned Hermissenda. Several signal transduction pathways have
been implicated in classical conditioning of this preparation, although
the major emphasis has been on protein kinase C. Here we provide
evidence for the activation and phosphorylation of a mitogen-activated protein kinase (MAPK) pathway by one-trial and multi-trial
conditioning. A one-trial in vitro conditioning
procedure consisting of light (CS) paired with the application of 5-HT
results in the increased incorporation of 32PO4
into proteins detected with two-dimensional gel electrophoresis. Two of
the phosphoproteins have molecular weights of 44 and 42 kDa, consistent
with extracellular signal-regulated protein kinases (ERK1 and ERK2).
Phosphorylation of the 44 and 42 kDa proteins by one-trial conditioning
was inhibited by pretreatment with PD098059, A MEK1 (ERK-Activating
kinase) inhibitor. Assays of ERK activity with brain myelin basic
protein as a substrate revealed greater ERK activity for the group that
received one-trial conditioning compared with an unpaired control
group. Western blot analysis of phosphorylated ERK using antibodies
recognizing the dually phosphorylated forms of ERK1 and ERK2 showed an
increase in phosphorylation after one-trial conditioning compared with
unpaired controls. The increased phosphorylation of ERK after one-trial
conditioning was blocked by pretreatment with PD098059.
Hermissenda that received 10 or 15 conditioning trials
showed significant behavioral suppression compared with pseudo-random
controls. After conditioning and behavioral testing, the conditioned
animals showed significantly greater phosphorylation of ERK compared
with the pseudo-random controls. These results show that the ERK-MAPK
signaling pathway is activated in Pavlovian conditioning of
Hermissenda.
Key words:
Pavlovian conditioning; MAPK pathway; signal
transduction; cellular excitability; ERK cascade; Hermissenda
Copyright © 1998 Society for Neuroscience 0270-6474/98/1893480-08$05.00/0
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