 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, January 1, 1999, 19(1):347-357
Cellular Traces of Behavioral Classical Conditioning Can Be Recorded at Several Specific Sites in a Simple Nervous System
Kevin Staras, György Kemenes, and Paul R. Benjamin Sussex Centre for Neuroscience, School of Biological Sciences, University of Sussex, Falmer, Brighton, United Kingdom BN1 9QG
We used a behavioral learning paradigm followed by electrophysiological analysis to find sites in the Lymnaea feeding network in which electrical changes could be recorded after appetitive conditioning. Specifically, we analyzed conditioning-induced changes in cellular responses in the mechanosensory conditioned stimulus (CS) pathway, in the central pattern generator (CPG) network, and in feeding motoneurons. During training, experimental animals received 15 pairings of lip touch (the CS) with sucrose (the unconditioned stimulus, US). Control animals received 15 random CS and US presentations. Electrophysiological tests on semi-intact preparations made from conditioned animals demonstrated a network correlate of the overall feeding conditioned response, a touch-evoked CPG-driven fictive feeding rhythm. At the motoneuronal level, we found significant conditioning-induced increases in the amplitude of an early touch-evoked EPSP and spike activity, recorded from the B3 feeding motoneuron. Increases in EPSP amplitude and motoneuronal spike activity could occur independently of conditioned fictive feeding. These changes in response recorded at the level of CPG interneurons, and motoneurons were preceded by changes recorded in the CS pathway. This was demonstrated by recording a conditioning-induced increase in the number of touch-evoked spikes in the cerebrobuccal connective, which forms part of the CS pathway. The finding that electrophysiological changes after conditioning can be recorded at multiple sites in this simple system provided an important intermediate level of analysis between whole animal behavior and cellular studies on the synaptic sites of plasticity.
Key words: classical conditioning; memory trace; cellular plasticity; feeding behavior; invertebrates; semi-intact preparations; Lymnaea
Copyright © 1999 Society for Neuroscience 0270-6474/99/191347-11$05.00/0
This article has been cited by other articles:
M. Diaz-Rios and M. W. Miller
Target-Specific Regulation of Synaptic Efficacy in the Feeding Central Pattern Generator of Aplysia: Potential Substrates for Behavioral Plasticity?
Biol. Bull., June 1, 2006; 210(3): 215 - 229.
[Abstract] [Full Text] [PDF]
V. A. Straub, B. J. Styles, J. S. Ireland, M. O'Shea, and P. R. Benjamin
Central localization of plasticity involved in appetitive conditioning in Lymnaea
Learn. Mem., November 1, 2004; 11(6): 787 - 793.
[Abstract] [Full Text] [PDF]
R. Mozzachiodi, H. A. Lechner, D. A. Baxter, and J. H. Byrne
In Vitro Analog of Classical Conditioning of Feeding Behavior in Aplysia
Learn. Mem., November 1, 2003; 10(6): 478 - 494.
[Abstract] [Full Text] [PDF]
K. Lukowiak, S. Sangha, C. McComb, N. Varshney, D. Rosenegger, H. Sadamoto, and A. Scheibenstock
Associative learning and memory in Lymnaea stagnalis: how well do they remember?
J. Exp. Biol., July 1, 2003; 206(13): 2097 - 2103.
[Abstract] [Full Text] [PDF]
G. E. Spencer, M. H. Kazmi, N. I. Syed, and K. Lukowiak
Changes in the Activity of a CPG Neuron After the Reinforcement of an Operantly Conditioned Behavior in Lymnaea
J Neurophysiol, October 1, 2002; 88(4): 1915 - 1923.
[Abstract] [Full Text] [PDF]
C. J. H. Elliott and A. J. Susswein
Comparative neuroethology of feeding control in molluscs
J. Exp. Biol., April 1, 2002; 205(7): 877 - 896.
[Abstract] [Full Text] [PDF]
I. Kemenes, G. Kemenes, R. J. Andrew, P. R. Benjamin, and M. O'Shea
Critical Time-Window for NO-cGMP-Dependent Long-Term Memory Formation after One-Trial Appetitive Conditioning
J. Neurosci., February 15, 2002; 22(4): 1414 - 1425.
[Abstract] [Full Text] [PDF]
R. Nargeot
Long-Lasting Reconfiguration of Two Interacting Networks by a Cooperation of Presynaptic and Postsynaptic Plasticity
J. Neurosci., May 1, 2001; 21(9): 3282 - 3294.
[Abstract] [Full Text] [PDF]
G. Kemenes, K. Staras, and P. R. Benjamin
Multiple Types of Control by Identified Interneurons in a Sensory-Activated Rhythmic Motor Pattern
J. Neurosci., April 15, 2001; 21(8): 2903 - 2911.
[Abstract] [Full Text] [PDF]
N. Jones, G. Kemenes, and P. R. Benjamin
Selective Expression of Electrical Correlates of Differential Appetitive Classical Conditioning in a Feeding Network
J Neurophysiol, January 1, 2001; 85(1): 89 - 97.
[Abstract] [Full Text] [PDF]
J. Haney and K. Lukowiak
Context Learning and the Effect of Context on Memory Retrieval in Lymnaea
Learn. Mem., January 1, 2001; 8(1): 35 - 43.
[Abstract] [Full Text]
H. A. Lechner, D. A. Baxter, and J. H. Byrne
Classical Conditioning of Feeding in Aplysia: I. Behavioral Analysis
J. Neurosci., May 1, 2000; 20(9): 3369 - 3376.
[Abstract] [Full Text] [PDF]
H. A. Lechner, D. A. Baxter, and J. H. Byrne
Classical Conditioning of Feeding in Aplysia: II. Neurophysiological Correlates
J. Neurosci., May 1, 2000; 20(9): 3377 - 3386.
[Abstract] [Full Text] [PDF]
P. R. Benjamin, K. Staras, and G. Kemenes
A Systems Approach to the Cellular Analysis of Associative Learning in the Pond Snail Lymnaea
Learn. Mem., May 1, 2000; 7(3): 124 - 131.
[Abstract] [Full Text]
K. Staras, G. Kemenes, and P. R. Benjamin
Electrophysiological and Behavioral Analysis of Lip Touch as a Component of the Food Stimulus in the Snail Lymnaea
J Neurophysiol, March 1, 1999; 81(3): 1261 - 1273.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|