QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (81) Citing Articles via Google Scholar Google Scholar Articles by Garwood, J. Articles by Faissner, A. Search for Related Content PubMed PubMed Citation Articles by Garwood, J. Articles by Faissner, A.

 Previous Article  |  Next Article 

The Journal of Neuroscience, May 15, 1999, 19(10):3888-3899

DSD-1-Proteoglycan Is the Mouse Homolog of Phosphacan and Displays Opposing Effects on Neurite Outgrowth Dependent on Neuronal Lineage

Jeremy Garwood¹, Oliver Schnädelbach¹, Albrecht Clement¹, Katrin Schütte¹, Alfred Bach³, and Andreas Faissner^{1, 2}

¹ Department of Neurobiology, University of Heidelberg, INF 364, 69120 Heidelberg, Germany, ² Laboratoire de Neurobiologie du Développement et de la Régénération (LNDR), UPR 1352 Centre de Neurochimie du Centre National de la Recherche Scientifique et Université Louis Pasteur (ULP), 67084 Strasbourg, France, and ³ BASF-LYNX Bioscience, INF 515, 69120 Heidelberg, Germany

DSD-1-PG is a chondroitin sulfate proteoglycan (CSPG) expressed by glial cells that can promote neurite outgrowth from rat embryonic mesencephalic (E14) and hippocampal (E18) neurons, an activity that is associated with the CS glycosaminoglycans (GAGs). Further characterization of DSD-1-PG has included sequencing of peptides from the core protein and the cloning of the corresponding cDNA using polyclonal antisera against DSD-1-PG to screen phage expression libraries. On the basis of these studies we have identified DSD-1-PG as the mouse homolog of phosphacan, a neural rat CSPG. Monoclonal antibodies 3H1 and 3F8 against carbohydrate residues on rat phosphacan recognize these epitopes on DSD-1-PG. The epitopes of the antibodies, L2/HNK-1 and L5/Lewis-X, which have been implicated in functional interactions, are also found on DSD-1-PG. Although DSD-1-PG has previously been shown to promote neurite outgrowth, its upregulation after stab wounding of the CNS and its localization in regions that are considered boundaries to axonal extension suggested that it may also have inhibitory functions. Neonatal dorsal root ganglion (DRG) explants grown on a rich supportive substrate (laminin) with and without DSD-1-PG were strikingly inhibited by the proteoglycan. The inhibitory effects of DSD-1-PG on the DRG explants were not relieved by removal of the CS GAGs, indicating that this activity is associated with the core glycoprotein. The neurite outgrowth from embryonic hippocampal neurons on laminin was not affected by the addition of DSD-1-PG. This indicates that DSD-1-PG/mouse phosphacan can have opposing effects on the process of neurite outgrowth dependent on neuronal lineage.

Key words: DSD-1-proteoglycan; phosphacan; chondroitin sulfate proteoglycan; neurite outgrowth; hippocampus; dorsal root ganglion

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/19103888-12$05.00/0

This article has been cited by other articles:

				A. Hurtado, H. Podinin, M. Oudega, and B. Grimpe Deoxyribozyme-mediated knockdown of xylosyltransferase-1 mRNA promotes axon growth in the adult rat spinal cord Brain, October 1, 2008; 131(10): 2596 - 2605. [Abstract] [Full Text] [PDF]

				H. Wang, Y. Katagiri, T. E. McCann, E. Unsworth, P. Goldsmith, Z.-X. Yu, F. Tan, L. Santiago, E. M. Mills, Y. Wang, et al. Chondroitin-4-sulfation negatively regulates axonal guidance and growth J. Cell Sci., September 15, 2008; 121(18): 3083 - 3091. [Abstract] [Full Text] [PDF]

				K. Akita, A. von Holst, Y. Furukawa, T. Mikami, K. Sugahara, and A. Faissner Expression of Multiple Chondroitin/Dermatan Sulfotransferases in the Neurogenic Regions of the Embryonic and Adult Central Nervous System Implies That Complex Chondroitin Sulfates Have a Role in Neural Stem Cell Maintenance Stem Cells, March 1, 2008; 26(3): 798 - 809. [Abstract] [Full Text] [PDF]

				S. Sirko, A. von Holst, A. Wizenmann, M. Gotz, and A. Faissner Chondroitin sulfate glycosaminoglycans control proliferation, radial glia cell differentiation and neurogenesis in neural stem/progenitor cells Development, August 1, 2007; 134(15): 2727 - 2738. [Abstract] [Full Text] [PDF]

				S. S Deepa, S. Yamada, S. Fukui, and K. Sugahara Structural determination of novel sulfated octasaccharides isolated from chondroitin sulfate of shark cartilage and their application for characterizing monoclonal antibody epitopes Glycobiology, June 1, 2007; 17(6): 631 - 645. [Abstract] [Full Text] [PDF]

				K. Nakanishi, S. Aono, K. Hirano, Y. Kuroda, M. Ida, Y. Tokita, F. Matsui, and A. Oohira Identification of Neurite Outgrowth-promoting Domains of Neuroglycan C, a Brain-specific Chondroitin Sulfate Proteoglycan, and Involvement of Phosphatidylinositol 3-Kinase and Protein Kinase C Signaling Pathways in Neuritogenesis J. Biol. Chem., August 25, 2006; 281(34): 24970 - 24978. [Abstract] [Full Text] [PDF]

				U. Zacharias and U. Rauch Competition and cooperation between tenascin-R, lecticans and contactin 1 regulate neurite growth and morphology. J. Cell Sci., August 15, 2006; 119(Pt 16): 3456 - 3466. [Abstract] [Full Text] [PDF]

				C. Mitsunaga, T. Mikami, S. Mizumoto, J. Fukuda, and K. Sugahara Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains in the Development of Cerebellum: SPATIOTEMPORAL REGULATION OF THE EXPRESSION OF CRITICAL DISULFATED DISACCHARIDES BY SPECIFIC SULFOTRANSFERASES J. Biol. Chem., July 14, 2006; 281(28): 18942 - 18952. [Abstract] [Full Text] [PDF]

				A. M. Tan, M. Colletti, A. T. Rorai, J. H. P. Skene, and J. M. Levine Antibodies against the NG2 proteoglycan promote the regeneration of sensory axons within the dorsal columns of the spinal cord. J. Neurosci., May 3, 2006; 26(18): 4729 - 4739. [Abstract] [Full Text] [PDF]

				A. von Holst, S. Sirko, and A. Faissner The Unique 473HD-Chondroitinsulfate Epitope Is Expressed by Radial Glia and Involved in Neural Precursor Cell Proliferation J. Neurosci., April 12, 2006; 26(15): 4082 - 4094. [Abstract] [Full Text] [PDF]

				X. Bao, T. Mikami, S. Yamada, A. Faissner, T. Muramatsu, and K. Sugahara Heparin-binding Growth Factor, Pleiotrophin, Mediates Neuritogenic Activity of Embryonic Pig Brain-derived Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains J. Biol. Chem., March 11, 2005; 280(10): 9180 - 9191. [Abstract] [Full Text] [PDF]

				M. Hikino, T. Mikami, A. Faissner, A.-C. E. S. Vilela-Silva, M. S. G. Pavao, and K. Sugahara Oversulfated Dermatan Sulfate Exhibits Neurite Outgrowth-promoting Activity toward Embryonic Mouse Hippocampal Neurons: IMPLICATIONS OF DERMATAN SULFATE IN NEURITOGENESIS IN THE BRAIN J. Biol. Chem., October 31, 2003; 278(44): 43744 - 43754. [Abstract] [Full Text] [PDF]

				N. Maeda, J. He, Y. Yajima, T. Mikami, K. Sugahara, and T. Yabe Heterogeneity of the Chondroitin Sulfate Portion of Phosphacan/6B4 Proteoglycan Regulates Its Binding Affinity for Pleiotrophin/Heparin Binding Growth-associated Molecule J. Biol. Chem., September 12, 2003; 278(37): 35805 - 35811. [Abstract] [Full Text] [PDF]

				J. Garwood, N. Heck, F. Reichardt, and A. Faissner Phosphacan Short Isoform, a Novel Non-proteoglycan Variant of Phosphacan/Receptor Protein Tyrosine Phosphatase-{beta}, Interacts with Neuronal Receptors and Promotes Neurite Outgrowth J. Biol. Chem., June 20, 2003; 278(26): 24164 - 24173. [Abstract] [Full Text] [PDF]

				S. S. Deepa, Y. Umehara, S. Higashiyama, N. Itoh, and K. Sugahara Specific Molecular Interactions of Oversulfated Chondroitin Sulfate E with Various Heparin-binding Growth Factors. IMPLICATIONS AS A PHYSIOLOGICAL BINDING PARTNER IN THE BRAIN AND OTHER TISSUES J. Biol. Chem., November 8, 2002; 277(46): 43707 - 43716. [Abstract] [Full Text] [PDF]

				R. A. Asher, D. A. Morgenstern, M. C. Shearer, K. H. Adcock, P. Pesheva, and J. W. Fawcett Versican Is Upregulated in CNS Injury and Is a Product of Oligodendrocyte Lineage Cells J. Neurosci., March 15, 2002; 22(6): 2225 - 2236. [Abstract] [Full Text] [PDF]

				C. G. Becker and T. Becker Repellent Guidance of Regenerating Optic Axons by Chondroitin Sulfate Glycosaminoglycans in Zebrafish J. Neurosci., February 1, 2002; 22(3): 842 - 853. [Abstract] [Full Text] [PDF]

				H. Ichijo and I. Kawabata Roles of the Telencephalic Cells and their Chondroitin Sulfate Proteoglycans in Delimiting an Anterior Border of the Retinal Pathway J. Neurosci., December 1, 2001; 21(23): 9304 - 9314. [Abstract] [Full Text] [PDF]

				L. Stepanek, Q. L. Sun, J. Wang, C. Wang, and J. L. Bixby CRYP-2/cPTPRO is a neurite inhibitory repulsive guidance cue for retinal neurons in vitro J. Cell Biol., August 20, 2001; 154(4): 867 - 878. [Abstract] [Full Text] [PDF]

				M. Inatani, M. Honjo, Y. Otori, A. Oohira, N. Kido, Y. Tano, Y. Honda, and H. Tanihara Inhibitory Effects of Neurocan and Phosphacan on Neurite Outgrowth from Retinal Ganglion Cells in Culture Invest. Ophthalmol. Vis. Sci., July 1, 2001; 42(8): 1930 - 1938. [Abstract] [Full Text] [PDF]

				M. Inatani, H. Tanihara, A. Oohira, Y. Otori, A. Nishida, M. Honjo, N. Kido, and Y. Honda Neuroglycan C, a Neural Tissue-Specific Transmembrane Chondroitin Sulfate Proteoglycan, in Retinal Neural Network Formation Invest. Ophthalmol. Vis. Sci., December 1, 2000; 41(13): 4338 - 4346. [Abstract] [Full Text]

				S. Harroch, M. Palmeri, J. Rosenbluth, A. Custer, M. Okigaki, P. Shrager, M. Blum, J. D. Buxbaum, and J. Schlessinger No Obvious Abnormality in Mice Deficient in Receptor Protein Tyrosine Phosphatase beta Mol. Cell. Biol., October 15, 2000; 20(20): 7706 - 7715. [Abstract] [Full Text]

				Y. P. Wu, C.-J. Siao, W. Lu, T.-C. Sung, M. A. Frohman, P. Milev, T. H. Bugge, J. L. Degen, J. M. Levine, R. U. Margolis, et al. The Tissue Plasminogen Activator (tPA)/Plasmin Extracellular Proteolytic System Regulates Seizure-induced Hippocampal Mossy Fiber Outgrowth through a Proteoglycan Substrate J. Cell Biol., March 20, 2000; 148(6): 1295 - 1304. [Abstract] [Full Text] [PDF]

				R. J. McKeon, M. J. Jurynec, and C. R. Buck The Chondroitin Sulfate Proteoglycans Neurocan and Phosphacan Are Expressed by Reactive Astrocytes in the Chronic CNS Glial Scar J. Neurosci., December 15, 1999; 19(24): 10778 - 10788. [Abstract] [Full Text] [PDF]

				C. Ueoka, N. Kaneda, I. Okazaki, S. Nadanaka, T. Muramatsu, and K. Sugahara Neuronal Cell Adhesion, Mediated by the Heparin-binding Neuroregulatory Factor Midkine, Is Specifically Inhibited by Chondroitin Sulfate E. STRUCTURAL AND FUNCTIONAL IMPLICATIONS OF THE OVER-SULFATED CHONDROITIN SULFATE J. Biol. Chem., November 22, 2000; 275(48): 37407 - 37413. [Abstract] [Full Text] [PDF]

				K. Uchimura, K. Kadomatsu, H. Nishimura, H. Muramatsu, E. Nakamura, N. Kurosawa, O. Habuchi, F. M. El-Fasakhany, Y. Yoshikai, and T. Muramatsu Functional Analysis of the Chondroitin 6-Sulfotransferase Gene in Relation to Lymphocyte Subpopulations, Brain Development, and Oversulfated Chondroitin Sulfates J. Biol. Chem., January 4, 2002; 277(2): 1443 - 1450. [Abstract] [Full Text] [PDF]

				K. Tsuchida, J. Shioi, S. Yamada, G. Boghosian, A. Wu, H. Cai, K. Sugahara, and N. K. Robakis Appican, the Proteoglycan Form of the Amyloid Precursor Protein, Contains Chondroitin Sulfate E in the Repeating Disaccharide Region and 4-O-Sulfated Galactose in the Linkage Region J. Biol. Chem., September 28, 2001; 276(40): 37155 - 37160. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help