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The Journal of Neuroscience, July 1, 1999, 19(13):5332-5347

The Native Rat Olfactory Cyclic Nucleotide-Gated Channel Is Composed of Three Distinct Subunits

Wolfgang Bönigk¹, Jonathan Bradley², Frank Müller¹, Federico Sesti¹, Ingrid Boekhoff³, Gabriele V. Ronnett⁴, U. Benjamin Kaupp¹, and Stephan Frings¹

¹ Forschungszentrum Jülich, Institut für Biologische Informationsverarbeitung, 52425 Jülich, Germany, ² Ecole Normale Supérieure, Laboratoire de Neurobiologie, 75005 Paris, France, ³ Universität Hohenheim, Institut für Physiologie, 70574 Stuttgart, Germany, and ⁴ Department of Neurosciences and Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

Cyclic nucleotide-gated (CNG) channels play central roles in visual and olfactory signal transduction. In the retina, rod photoreceptors express the subunits CNC1 and CNC1a. In cone photoreceptors, only CNC2 expression has been demonstrated so far. Rat olfactory sensory neurons (OSNs) express two homologous subunits, here designated CNC3 and CNC4. This paper describes the characterization of CNC1b, a third subunit expressed in OSNs and establishes it as a component of the native channel. CNC1b is an alternate splice form of the rod photoreceptor CNC1a subunit. Analysis of mRNA and protein expression together suggest co-expression of all three subunits in sensory cilia of OSNs. From single-channel analyses of native rat olfactory channels and of channels expressed heterologously from all possible combinations of the CNC3, -4, and -1b subunits, we conclude that the native CNG channel in OSNs is composed of all three subunits. Thus, CNG channels in both rod photoreceptors and olfactory sensory neurons result from coassembly of specific  subunits with various forms of an alternatively spliced  subunit.

Key words: cAMP; cGMP; sensory transduction; olfaction; ion channels; channel structure; subunit

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Copyright © 1999 Society for Neuroscience  0270-6474/99/19135332-16$05.00/0

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