QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (45) Citing Articles via Google Scholar Google Scholar Articles by Rubio, M. E. Articles by Wenthold, R. J. Search for Related Content PubMed PubMed Citation Articles by Rubio, M. E. Articles by Wenthold, R. J.

 Previous Article  |  Next Article 

The Journal of Neuroscience, July 1, 1999, 19(13):5549-5562

Differential Distribution of Intracellular Glutamate Receptors in Dendrites

Maria E. Rubio and Robert J. Wenthold

Laboratory of Neurochemistry, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, Maryland 20892

Glutamate receptors are synthesized in the cell body and transported in intracellular compartments to the target synapse. The objective of the present study was to analyze the intracellular pool of glutamate receptors and determine whether the intracellular pool was related to the synaptic distribution of the receptors. As a model system, we chose the fusiform cell of the dorsal cochlear nucleus for which we have previously demonstrated that receptors are selectively targeted to synapses on apical and basal dendrites. A combination of retrograde tracing and postembedding immunogold labeling was used to quantify intracellular receptors in segments of apical and basal dendrites. Immunolabeling for GluR4 and mGluR1 is present at synapses on basal dendrites but not on apical dendrites, whereas immunolabeling for GluR2/3 is present at both populations of synapses. In the analysis of intracellular pools, we find that GluR2/3 is equally distributed in apical and basal dendrites, whereas GluR4 and mGluR1 are more concentrated in basal dendrites than in apical dendrites. These findings indicate that the distribution of intracellular receptors is related to that of synaptic receptors and suggest that a mechanism exists in neurons to target proteins to dendritic domains soon after synthesis. We found no evidence for the existence of a pool of intracellular receptors, which could represent a receptor reserve, near the postsynaptic density. Receptors were often found in clusters associated with tubulovesicular membranes of the endoplasmic reticulum, identified with immunoglobulin binding protein (BIP) or calnexin, suggesting that this organelle is involved in receptor transport in dendrites.

Key words: glutamate receptor; immunocytochemistry; dendrite; postembedding; targeting; cochlear nucleus

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/19135549-14$05.00/0

This article has been cited by other articles:

				O. A. Ramirez, R. L. Vidal, J. A. Tello, K. J. Vargas, S. Kindler, S. Hartel, and A. Couve Dendritic Assembly of Heteromeric {gamma}-Aminobutyric Acid Type B Receptor Subunits in Hippocampal Neurons J. Biol. Chem., May 8, 2009; 284(19): 13077 - 13085. [Abstract] [Full Text] [PDF]

				Y. Zhao, M. E. Rubio, and T. Tzounopoulos Distinct Functional and Anatomical Architecture of the Endocannabinoid System in the Auditory Brainstem J Neurophysiol, May 1, 2009; 101(5): 2434 - 2446. [Abstract] [Full Text] [PDF]

				H. Buff, A. C. Smith, and C. A. Korey Genetic Modifiers of Drosophila Palmitoyl-Protein Thioesterase 1-Induced Degeneration Genetics, May 1, 2007; 176(1): 209 - 220. [Abstract] [Full Text] [PDF]

				S. Oray, A. Majewska, and M. Sur Effects of Synaptic Activity on Dendritic Spine Motility of Developing Cortical Layer V Pyramidal Neurons Cereb Cortex, May 1, 2006; 16(5): 730 - 741. [Abstract] [Full Text] [PDF]

				K. Matsui, C. E. Jahr, and M. E. Rubio High-Concentration Rapid Transients of Glutamate Mediate Neural-Glial Communication via Ectopic Release J. Neurosci., August 17, 2005; 25(33): 7538 - 7547. [Abstract] [Full Text] [PDF]

				J. R. Cooney, J. L. Hurlburt, D. K. Selig, K. M. Harris, and J. C. Fiala Endosomal Compartments Serve Multiple Hippocampal Dendritic Spines from a Widespread Rather Than a Local Store of Recycling Membrane J. Neurosci., March 15, 2002; 22(6): 2215 - 2224. [Abstract] [Full Text] [PDF]

				J. Z. Kieval, G. W. Hubert, A. Charara, J.-F. Pare, and Y. Smith Subcellular and Subsynaptic Localization of Presynaptic and Postsynaptic Kainate Receptor Subunits in the Monkey Striatum J. Neurosci., November 15, 2001; 21(22): 8746 - 8757. [Abstract] [Full Text] [PDF]

				N. Sans, C. Racca, R. S. Petralia, Y.-X. Wang, J. McCallum, and R. J. Wenthold Synapse-Associated Protein 97 Selectively Associates with a Subset of AMPA Receptors Early in their Biosynthetic Pathway J. Neurosci., October 1, 2001; 21(19): 7506 - 7516. [Abstract] [Full Text] [PDF]

				G. W. Hubert, M. Paquet, and Y. Smith Differential Subcellular Localization of mGluR1a and mGluR5 in the Rat and Monkey Substantia Nigra J. Neurosci., March 15, 2001; 21(6): 1838 - 1847. [Abstract] [Full Text] [PDF]

				M. E. Rubio and F. Soto Distinct Localization of P2X Receptors at Excitatory Postsynaptic Specializations J. Neurosci., January 15, 2001; 21(2): 641 - 653. [Abstract] [Full Text] [PDF]

				G. Broutman and M. Baudry Involvement of the Secretory Pathway for AMPA Receptors in NMDA-Induced Potentiation in Hippocampus J. Neurosci., January 1, 2001; 21(1): 27 - 34. [Abstract] [Full Text] [PDF]

				F. Ango, J.-P. Pin, J. C. Tu, B. Xiao, P. F. Worley, J. Bockaert, and L. Fagni Dendritic and Axonal Targeting of Type 5 Metabotropic Glutamate Receptor Is Regulated by Homer1 Proteins and Neuronal Excitation J. Neurosci., December 1, 2000; 20(23): 8710 - 8716. [Abstract] [Full Text] [PDF]

				T. Opitz, S. Y. Grooms, M. V. L. Bennett, and R. S. Zukin Remodeling of alpha -amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor subunit composition in hippocampal neurons after global ischemia PNAS, November 21, 2000; 97(24): 13360 - 13365. [Abstract] [Full Text] [PDF]

				J. H. Caldwell, K. L. Schaller, R. S. Lasher, E. Peles, and S. R. Levinson Sodium channel Nav1.6 is localized at nodes of Ranvier, dendrites, and synapses PNAS, May 9, 2000; 97(10): 5616 - 5620. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help