 |
||||
|
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, July 1, 1999, 19(13):5619-5631
Actions of Brain-Derived Neurotrophic Factor in Slices from Rats with Spontaneous Seizures and Mossy Fiber Sprouting in the Dentate Gyrus
Helen E. Scharfman1, 2, Jeffrey H. Goodman1, and Anne L. Sollas1 1 Neurology Research Center, Helen Hayes Hospital, West Haverstraw, New York 10993-1195, and 2 Departments of Pharmacology and Neurology, Columbia University, College of Physicians and Surgeons, New York, New York 10032
This study examined the acute actions of brain-derived neurotrophic factor (BDNF) in the rat dentate gyrus after seizures, because previous studies have shown that BDNF has acute effects on dentate granule cell synaptic transmission, and other studies have demonstrated that BDNF expression increases in granule cells after seizures.
Pilocarpine-treated rats were studied because they not only have seizures and increased BDNF expression in granule cells, but they also have reorganization of granule cell "mossy fiber" axons. This reorganization, referred to as "sprouting," involves collaterals that grow into novel areas, i.e., the inner molecular layer, where granule cell and interneuron dendrites are located. Thus, this animal model allowed us to address the effects of BDNF in the dentate gyrus after seizures, as well as the actions of BDNF on mossy fiber transmission after reorganization.
In slices with sprouting, BDNF bath application enhanced responses recorded in the inner molecular layer to mossy fiber stimulation. Spontaneous bursts of granule cells occurred, and these were apparently generated at the site of the sprouted axon plexus. These effects were not accompanied by major changes in perforant path-evoked responses or paired-pulse inhibition, occurred only after prolonged (30-60 min) exposure to BDNF, and were blocked by K252a.
The results suggest a preferential action of BDNF at mossy fiber synapses, even after substantial changes in the dentate gyrus network. Moreover, the results suggest that activation of trkB receptors could contribute to the hyperexcitability observed in animals with sprouting. Because human granule cells also express increased BDNF mRNA after seizures, and sprouting can occur in temporal lobe epileptics, the results may have implications for understanding temporal lobe epilepsy.
Key words: neurotrophin; growth factor; hippocampus; epilepsy; glutamate; tyrosine kinase
Copyright © 1999 Society for Neuroscience 0270-6474/99/19135619-13$05.00/0
This article has been cited by other articles:
M. Ahn, D. Beacham, R. E. Westenbroek, T. Scheuer, and W. A. Catterall
Regulation of NaV1.2 Channels by Brain-Derived Neurotrophic Factor, TrkB, and Associated Fyn Kinase
J. Neurosci., October 24, 2007; 27(43): 11533 - 11542.
[Abstract] [Full Text] [PDF]
J. A. Gorter, E. A. van Vliet, E. Aronica, T. Breit, H. Rauwerda, F. H. Lopes da Silva, and W. J. Wadman
Potential New Antiepileptogenic Targets Indicated by Microarray Analysis in a Rat Model for Temporal Lobe Epilepsy
J. Neurosci., October 25, 2006; 26(43): 11083 - 11110.
[Abstract] [Full Text] [PDF]
R. Koyama and Y. Ikegaya
To BDNF or Not to BDNF: That Is the Epileptic Hippocampus
Neuroscientist, August 1, 2005; 11(4): 282 - 287.
[Abstract] [PDF]
L. Givalois, S. Arancibia, G. Alonso, and L. Tapia-Arancibia
Expression of Brain-Derived Neurotrophic Factor and Its Receptors in the Median Eminence Cells with Sensitivity to Stress
Endocrinology, October 1, 2004; 145(10): 4737 - 4747.
[Abstract] [Full Text] [PDF]
E. Tongiorgi, M. Armellin, P. G. Giulianini, G. Bregola, S. Zucchini, B. Paradiso, O. Steward, A. Cattaneo, and M. Simonato
Brain-Derived Neurotrophic Factor mRNA and Protein Are Targeted to Discrete Dendritic Laminas by Events That Trigger Epileptogenesis
J. Neurosci., July 28, 2004; 24(30): 6842 - 6852.
[Abstract] [Full Text] [PDF]
H. E. Scharfman, T. C. Mercurio, J. H. Goodman, M. A. Wilson, and N. J. MacLusky
Hippocampal Excitability Increases during the Estrous Cycle in the Rat: A Potential Role for Brain-Derived Neurotrophic Factor
J. Neurosci., December 17, 2003; 23(37): 11641 - 11652.
[Abstract] [Full Text] [PDF]
H. E. Scharfman, A. L. Sollas, R. E. Berger, and J. H. Goodman
Electrophysiological Evidence of Monosynaptic Excitatory Transmission Between Granule Cells After Seizure-Induced Mossy Fiber Sprouting
J Neurophysiol, October 1, 2003; 90(4): 2536 - 2547.
[Abstract] [Full Text] [PDF]
C. Rivera, H. Li, J. Thomas-Crusells, H. Lahtinen, T. Viitanen, A. Nanobashvili, Z. Kokaia, M. S. Airaksinen, J. Voipio, K. Kaila, et al.
BDNF-induced TrkB activation down-regulates the K+-Cl- cotransporter KCC2 and impairs neuronal Cl- extrusion
J. Cell Biol., December 9, 2002; 159(5): 747 - 752.
[Abstract] [Full Text] [PDF]
H. E. Scharfman
Book Review: Epilepsy as an Example of Neural Plasticity
Neuroscientist, April 1, 2002; 8(2): 154 - 173.
[Abstract] [PDF]
H. E. Scharfman, J. H. Goodman, and A. L. Sollas
Granule-Like Neurons at the Hilar/CA3 Border after Status Epilepticus and Their Synchrony with Area CA3 Pyramidal Cells: Functional Implications of Seizure-Induced Neurogenesis
J. Neurosci., August 15, 2000; 20(16): 6144 - 6158.
[Abstract] [Full Text] [PDF]
M. M. Bolton, A. J. Pittman, and D. C. Lo
Brain-Derived Neurotrophic Factor Differentially Regulates Excitatory and Inhibitory Synaptic Transmission in Hippocampal Cultures
J. Neurosci., May 1, 2000; 20(9): 3221 - 3232.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|