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The Journal of Neuroscience, July 15, 1999, 19(14):5802-5809

Directing Gene Expression to Gustducin-Positive Taste Receptor Cells

Gwendolyn T. Wong, Luis Ruiz-Avila, and Robert F. Margolskee

Howard Hughes Medical Institute, Department of Physiology and Biophysics, The Mount Sinai School of Medicine, New York, New York 10029

We have demonstrated that an 8.4 kb segment (GUS_8.4) from the upstream region of the mouse -gustducin gene acts as a fully functional promoter to target lacZ transgene expression to the gustducin-positive subset of taste receptor cells (TRCs). The GUS_8.4 promoter drove TRC expression of the -galactosidase marker at high levels and in a developmentally appropriate pattern. The gustducin minimal 1.4 kb promoter (GUS_1.4) by itself was insufficient to specify TRC expression. We also identified an upstream enhancer from the distal portion of the murine gustducin gene that, in combination with the minimal promoter, specified TRC expression of transgenes. Expression of the lacZ transgene from the GUS_8.4 promoter and of endogenous gustducin was coordinately lost after nerve section and simultaneously recovered after reinnervation, confirming the functionality of this promoter. Transgenic expression of rat -gustducin restored responsiveness of gustducin null mice to both bitter and sweet compounds, demonstrating the utility of the gustducin promoter.

Key words: gustducin; taste receptor cells; guanine nucleotide binding regulatory proteins; gustation; transgenic mice; promoter identification; -galactosidase

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Copyright © 1999 Society for Neuroscience  0270-6474/99/19145802-08$05.00/0

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