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The Journal of Neuroscience, July 15, 1999, 19(14):5990-6005
Site-Specific Migration and Neuronal Differentiation of Human Neural Progenitor Cells after Transplantation in the Adult Rat Brain
Rosemary A. Fricker1, 2, Melissa K. Carpenter3, 4, Christian Winkler1, Corinne Greco3, Monte A. Gates1, 2, and Anders Björklund1 1 Wallenberg Neuroscience Center, Division of Neurobiology, Lund University, S-223 Lund, Sweden, 2 Department of Neurology, Harvard Medical School, Children's Hospital, Boston, Massachusetts 02115, 3 CytoTherapeutics, Lincoln, Rhode Island 02865, and 4 Geron Corporation, Menlo Park, California 94025
Neural progenitor cells obtained from the embryonic human forebrain were expanded up to 107-fold in culture in the presence of epidermal growth factor, basic fibroblast growth factor, and leukemia inhibitory growth factor. When transplanted into neurogenic regions in the adult rat brain, the subventricular zone, and hippocampus, the in vitro propagated cells migrated specifically along the routes normally taken by the endogenous neuronal precursors: along the rostral migratory stream to the olfactory bulb and within the subgranular zone in the dentate gyrus, and exhibited site-specific neuronal differentiation in the granular and periglomerular layers of the bulb and in the dentate granular cell layer. The cells exhibited substantial migration also within the non-neurogenic region, the striatum, in a seemingly nondirected manner up to ~1-1.5 mm from the graft core, and showed differentiation into both neuronal and glial phenotypes. Only cells with glial-like features migrated over longer distances within the mature striatum, whereas the cells expressing neuronal phenotypes remained close to the implantation site. The ability of the human neural progenitors to respond in vivo to guidance cues and signals that can direct their differentiation along multiple phenotypic pathways suggests that they can provide a powerful and virtually unlimited source of cells for experimental and clinical transplantation.
Key words: progenitor cells; human; transplantation; neuron; subventricular zone; dentate gyrus; striatum
Copyright © 1999 Society for Neuroscience 0270-6474/99/19145990-16$05.00/0
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