 |
 Previous Article | Next Article 
The Journal of Neuroscience, August 1, 1999, 19(15):6519-6527
Cloning and Characterization of Neuropilin-1-Interacting
Protein: A PSD-95/Dlg/ZO-1 Domain-Containing Protein That
Interacts with the Cytoplasmic Domain of Neuropilin-1
Huaibin
Cai1, 2 and
Randall R.
Reed1, 2, 3
1 Howard Hughes Medical Institutes and Departments of
2 Neuroscience and 3 Molecular Biology and
Genetics, The Johns Hopkins University School of Medicine, Baltimore,
Maryland 21205
Neuropilin-1 (Npn-1), a receptor for semaphorin III, mediates the
guidance of growth cones on extending neurites. The molecular mechanism
of Npn-1 signaling remains unclear. We have used a yeast two-hybrid
system to isolate a protein that interacts with the cytoplasmic domain
of Npn-1. This Npn-1-interacting protein (NIP) contains a central
PSD-95/Dlg/ZO-1 (PDZ) domain and a C-terminal acyl carrier
protein domain. The physiological interaction of Npn-1 and NIP is
supported by co-immunoprecipitation of these two proteins in extracts
from a heterologous expression system and from a native tissue. The
C-terminal three amino acids of Npn-1 (S-E-A-COOH), which is conserved
from Xenopus to human, is responsible for interaction
with the PDZ domain-containing C-terminal two-thirds of NIP. NIP as
well as Npn-1 are broadly expressed in mice as assayed by Northern and
Western analysis. Immunohistochemistry and in situ
hybridization experiments revealed that NIP expression overlaps with
that of Npn-1. NIP has been independently cloned as
RGS-GAIP-interacting protein (GIPC), where it was identified by
virtue of its interaction with the C terminus of RGS-GAIP and
suggested to participate in clathrin-coated vesicular trafficking. We
suggest that NIP and GIPC may participate in regulation of
Npn-1-mediated signaling as a molecular adapter that couples Npn-1 to
membrane trafficking machinery in the dynamic axon growth cone.
Key words:
neuropilin-1; PDZ domain; axon guidance; yeast
two-hybrid; neuron development; adapter protein; signal
transduction
Copyright © 1999 Society for Neuroscience 0270-6474/99/19156519-09$05.00/0
This article has been cited by other articles:
|
|
|
|
 
P. Gaur, D. R. Bielenberg, S. Samuel, D. Bose, Y. Zhou, M. J. Gray, N. A. Dallas, F. Fan, L. Xia, J. Lu, et al.
Role of Class 3 Semaphorins and Their Receptors in Tumor Growth and Angiogenesis
Clin. Cancer Res.,
November 15, 2009;
15(22):
6763 - 6770.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Ruiz de Almodovar, D. Lambrechts, M. Mazzone, and P. Carmeliet
Role and Therapeutic Potential of VEGF in the Nervous System
Physiol Rev,
April 1, 2009;
89(2):
607 - 648.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Larrivee, C. Freitas, S. Suchting, I. Brunet, and A. Eichmann
Guidance of Vascular Development: Lessons From the Nervous System
Circ. Res.,
February 27, 2009;
104(4):
428 - 441.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. A. Dallas, M. J. Gray, L. Xia, F. Fan, G. van Buren II, P. Gaur, S. Samuel, S. J. Lim, T. Arumugam, V. Ramachandran, et al.
Neuropilin-2-Mediated Tumor Growth and Angiogenesis in Pancreatic Adenocarcinoma
Clin. Cancer Res.,
December 15, 2008;
14(24):
8052 - 8060.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kawamura, X. Li, K. Goishi, L. A. van Meeteren, L. Jakobsson, S. Cebe-Suarez, A. Shimizu, D. Edholm, K. Ballmer-Hofer, L. Kjellen, et al.
Neuropilin-1 in regulation of VEGF-induced activation of p38MAPK and endothelial cell organization
Blood,
November 1, 2008;
112(9):
3638 - 3649.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Prahst, M. Heroult, A. A. Lanahan, N. Uziel, O. Kessler, N. Shraga-Heled, M. Simons, G. Neufeld, and H. G. Augustin
Neuropilin-1-VEGFR-2 Complexing Requires the PDZ-binding Domain of Neuropilin-1
J. Biol. Chem.,
September 12, 2008;
283(37):
25110 - 25114.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Salikhova, L. Wang, A. A. Lanahan, M. Liu, M. Simons, W. P. J. Leenders, D. Mukhopadhyay, and A. Horowitz
Vascular Endothelial Growth Factor and Semaphorin Induce Neuropilin-1 Endocytosis via Separate Pathways
Circ. Res.,
September 12, 2008;
103(6):
e71 - e79.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Walz, P. Feinstein, M. Khan, and P. Mombaerts
Axonal wiring of guanylate cyclase-D-expressing olfactory neurons is dependent on neuropilin 2 and semaphorin 3F
Development,
November 15, 2007;
134(22):
4063 - 4072.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Yi, R. S. Petralia, Z. Fu, C. C. Swanwick, Y.-X. Wang, K. Prybylowski, N. Sans, S. Vicini, and R. J. Wenthold
The Role of the PDZ Protein GIPC in Regulating NMDA Receptor Trafficking
J. Neurosci.,
October 24, 2007;
27(43):
11663 - 11675.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. W. Vander Kooi, M. A. Jusino, B. Perman, D. B. Neau, H. D. Bellamy, and D. J. Leahy
Structural basis for ligand and heparin binding to neuropilin B domains
PNAS,
April 10, 2007;
104(15):
6152 - 6157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. A. Lanahan, T. W. Chittenden, E. Mulvihill, K. Smith, S. Schwartz, and M. Simons
Synectin-dependent gene expression in endothelial cells
Physiol Genomics,
November 21, 2006;
27(3):
380 - 390.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Lai, C. Xie, S. G. McCormack, H.-C. Chiang, M. K. Michalak, X. Lin, J. Chandran, H. Shim, M. Shimoji, M. R. Cookson, et al.
Amyotrophic Lateral Sclerosis 2-Deficiency Leads to Neuronal Degeneration in Amyotrophic Lateral Sclerosis through Altered AMPA Receptor Trafficking.
J. Neurosci.,
November 8, 2006;
26(45):
11798 - 11806.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wu, M. O'Donnell, A. D. Gitler, and P. S. Klein
Kermit 2/XGIPC, an IGF1 receptor interacting protein, is required for IGF signaling in Xenopus eye development
Development,
September 15, 2006;
133(18):
3651 - 3660.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. N. Naccache, T. Hasson, and A. Horowitz
Binding of internalized receptors to the PDZ domain of GIPC/synectin recruits myosin VI to endocytic vesicles
PNAS,
August 22, 2006;
103(34):
12735 - 12740.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Ghez, Y. Lepelletier, S. Lambert, J.-M. Fourneau, V. Blot, S. Janvier, B. Arnulf, P. M. van Endert, N. Heveker, C. Pique, et al.
Neuropilin-1 Is Involved in Human T-Cell Lymphotropic Virus Type 1 Entry
J. Virol.,
July 15, 2006;
80(14):
6844 - 6854.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Wang, D. Mukhopadhyay, and X. Xu
C terminus of RGS-GAIP-interacting protein conveys neuropilin-1-mediated signaling during angiogenesis
FASEB J,
July 1, 2006;
20(9):
1513 - 1515.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Fu, X. Huang, H. Zhong, R. M. Mortensen, L. G. D'Alecy, and R. R. Neubig
Endogenous RGS Proteins and G{alpha} Subtypes Differentially Control Muscarinic and Adenosine-Mediated Chronotropic Effects
Circ. Res.,
March 17, 2006;
98(5):
659 - 666.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. C. Reed, C. Cefalu, B. H. Bellaire, J. A. Cardelli, T. Louis, J. Salamon, M. A. Bloecher, and R. C. Bunn
GLUT1CBP(TIP2/GIPC1) Interactions with GLUT1 and Myosin VI: Evidence Supporting an Adapter Function for GLUT1CBP
Mol. Biol. Cell,
September 1, 2005;
16(9):
4183 - 4201.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Favre-Bonvin, C. Reynaud, C. Kretz-Remy, and P. Jalinot
Human Papillomavirus Type 18 E6 Protein Binds the Cellular PDZ Protein TIP-2/GIPC, Which Is Involved in Transforming Growth Factor {beta} Signaling and Triggers Its Degradation by the Proteasome
J. Virol.,
April 1, 2005;
79(7):
4229 - 4237.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Hoeben, B. Landuyt, M. S. Highley, H. Wildiers, A. T. Van Oosterom, and E. A. De Bruijn
Vascular Endothelial Growth Factor and Angiogenesis
Pharmacol. Rev.,
December 1, 2004;
56(4):
549 - 580.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Cheng, H. Jia, M. Lohr, A. Bagherzadeh, D. I. R. Holmes, D. Selwood, and I. Zachary
Anti-chemorepulsive Effects of Vascular Endothelial Growth Factor and Placental Growth Factor-2 in Dorsal Root Ganglion Neurons Are Mediated via Neuropilin-1 and Cyclooxygenase-derived Prostanoid Production
J. Biol. Chem.,
July 16, 2004;
279(29):
30654 - 30661.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Jeanneteau, J. Diaz, P. Sokoloff, and N. Griffon
Interactions of GIPC with Dopamine D2, D3 but not D4 Receptors Define a Novel Mode of Regulation of G Protein-coupled Receptors
Mol. Biol. Cell,
February 1, 2004;
15(2):
696 - 705.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Wang, H. Zeng, P. Wang, S. Soker, and D. Mukhopadhyay
Neuropilin-1-mediated Vascular Permeability Factor/Vascular Endothelial Growth Factor-dependent Endothelial Cell Migration
J. Biol. Chem.,
December 5, 2003;
278(49):
48848 - 48860.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hirakawa, C. Galet, M. Kishi, and M. Ascoli
GIPC Binds to the Human Lutropin Receptor (hLHR) through an Unusual PDZ Domain Binding Motif, and It Regulates the Sorting of the Internalized Human Choriogonadotropin and the Density of Cell Surface hLHR
J. Biol. Chem.,
December 5, 2003;
278(49):
49348 - 49357.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hasson
Myosin VI: two distinct roles in endocytosis
J. Cell Sci.,
September 1, 2003;
116(17):
3453 - 3461.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. K. Atwal, K. K. Singh, M. Tessier-Lavigne, F. D. Miller, and D. R. Kaplan
Semaphorin 3F Antagonizes Neurotrophin-Induced Phosphatidylinositol 3-Kinase and Mitogen-Activated Protein Kinase Kinase Signaling: A Mechanism for Growth Cone Collapse
J. Neurosci.,
August 20, 2003;
23(20):
7602 - 7609.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Hu, W. Chen, N. P. Martin, E. J. Whalen, R. T. Premont, and R. J. Lefkowitz
GIPC Interacts with the {beta}1-Adrenergic Receptor and Regulates {beta}1-Adrenergic Receptor-mediated ERK Activation
J. Biol. Chem.,
July 3, 2003;
278(28):
26295 - 26301.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Hollinger and J. R. Hepler
Cellular Regulation of RGS Proteins: Modulators and Integrators of G Protein Signaling
Pharmacol. Rev.,
September 1, 2002;
54(3):
527 - 559.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Lou, T. McQuistan, R. A. Orlando, and M. G. Farquhar
GAIP, GIPC and G{alpha}i3 are Concentrated in Endocytic Compartments of Proximal Tubule Cells: Putative Role in Regulating Megalin's Function
J. Am. Soc. Nephrol.,
April 1, 2002;
13(4):
918 - 927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. He, K. C. Wang, V. Koprivica, G. Ming, and H.-J. Song
Knowing How to Navigate: Mechanisms of Semaphorin Signaling in the Nervous System
Sci. Signal.,
February 12, 2002;
2002(119):
re1 - re1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. C. Blobe, X. Liu, S. J. Fang, T. How, and H. F. Lodish
A Novel Mechanism for Regulating Transforming Growth Factor beta (TGF-beta ) Signaling. FUNCTIONAL MODULATION OF TYPE III TGF-beta RECEPTOR EXPRESSION THROUGH INTERACTION WITH THE PDZ DOMAIN PROTEIN, GIPC
J. Biol. Chem.,
October 19, 2001;
276(43):
39608 - 39617.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Tan, M. A. Deardorff, J.-P. Saint-Jeannet, J. Yang, A. Arzoumanian, and P. S. Klein
Kermit, a frizzled interacting protein, regulates frizzled 3 signaling in neural crest development
Development,
October 1, 2001;
128(19):
3665 - 3674.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Lou, H. Yano, F. Lee, M. V. Chao, and M. G. Farquhar
GIPC and GAIP Form a Complex with TrkA: A Putative Link between G Protein and Receptor Tyrosine Kinase Pathways
Mol. Biol. Cell,
March 1, 2001;
12(3):
615 - 627.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. Pavelock, K. M. Braas, L'H. Ouafik, G. Osol, and V. May
Differential Expression and Regulation of the Vascular Endothelial Growth Factor Receptors Neuropilin-1 and Neuropilin-2 in Rat Uterus
Endocrinology,
February 1, 2001;
142(2):
613 - 622.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Robinson and S. Stringer
The splice variants of vascular endothelial growth factor (VEGF) and their receptors
J. Cell Sci.,
January 3, 2001;
114(5):
853 - 865.
[Abstract]
[PDF]
|
|
|
|
|
|
|
G. Fuh, K. C. Garcia, and A. M. de Vos
The Interaction of Neuropilin-1 with Vascular Endothelial Growth Factor and Its Receptor Flt-1
J. Biol. Chem.,
August 25, 2000;
275(35):
26690 - 26695.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Inagaki, Y. Ohoka, H. Sugimoto, S. Fujioka, M. Amazaki, H. Kurinami, N. Miyazaki, M. Tohyama, and T. Furuyama
Sema4C, a Transmembrane Semaphorin, Interacts with a Post-synaptic Density Protein, PSD-95
J. Biol. Chem.,
March 16, 2001;
276(12):
9174 - 9181.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. B. Whitaker, B. J. Limberg, and J. S. Rosenbaum
Vascular Endothelial Growth Factor Receptor-2 and Neuropilin-1 Form a Receptor Complex That Is Responsible for the Differential Signaling Potency of VEGF165 and VEGF121
J. Biol. Chem.,
June 29, 2001;
276(27):
25520 - 25531.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ligensa, S. Krauss, D. Demuth, R. Schumacher, J. Camonis, G. Jaques, and K. M. Weidner
A PDZ Domain Protein Interacts with the C-terminal Tail of the Insulin-like Growth Factor-1 Receptor but Not with the Insulin Receptor
J. Biol. Chem.,
August 31, 2001;
276(36):
33419 - 33427.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Gluzman-Poltorak, T. Cohen, Y. Herzog, and G. Neufeld
Neuropilin-2 and Neuropilin-1 Are Receptors for the 165-Amino Acid Form of Vascular Endothelial Growth Factor (VEGF) and of Placenta Growth Factor-2, but Only Neuropilin-2 Functions as a Receptor for the 145-Amino Acid Form of VEGF
J. Biol. Chem.,
June 9, 2000;
275(24):
18040 - 18045.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
