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Previous Article | Next Article
The Journal of Neuroscience, August 1, 1999, 19(15):6599-6609
Smell and Taste Perception in Drosophila melanogaster Larva: Toxin Expression Studies in Chemosensory Neurons
Gertrud Heimbeck, Véronique Bugnon, Nanaë Gendre, Corinne Häberlin, and Reinhard F. Stocker Institute of Zoology and Program in Neuroscience, University of Fribourg, CH-1700 Fribourg, Switzerland
GAL4-driven targeted expression of tetanus toxin light chain (UAS-TeTxLC) in a subset of chemosensory neurons of the larval antennomaxillary complex (AMC) and pharynx causes abnormal chemosensory behavior in Drosophila melanogaster. Consistent with strongest staining in the dorsal organ (DO), the presumed olfactory organ of the AMC, tetanus toxin-expressing larvae subjected to an olfactory preference assay show anosmic behavior to most volatile substances tested. Furthermore, we observed reduced responses to sodium chloride, fructose, and sucrose in gustatory plate assays. Surprisingly, the entire subset of labeled sensory neurons from the terminal (maxillary) organ (TO) of the AMC was found to project via the antennal nerve to the larval antennal lobe region. The maxillary nerve remained completely unstained. Hence, a subset of neurons from the TO builds an anatomical entity with projections from the DO. Our results suggest that the AMC contains both olfactory and gustatory sensilla, and that the DO is the main olfactory organ in larvae.
Key words: Drosophila melanogaster; insect; larva; antennomaxillary complex; GAL4 enhancer trap line; tetanus toxin expression; smell; taste; behavior
Copyright © 1999 Society for Neuroscience 0270-6474/99/19156599-11$05.00/0
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