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The Journal of Neuroscience, August 15, 1999, 19(16):7048-7056
Netrin UNC-6 and the Regulation of Branching and Extension of
Motoneuron Axons from the Ventral Nerve Cord of
Caenorhabditis elegans
Yoo-Shick
Lim,
Smita
Mallapur,
Gautam
Kao,
Xing-Cong
Ren, and
William G.
Wadsworth
Department of Pathology, Robert Wood Johnson Medical School,
Piscataway, New Jersey 08854-5635
In the Caenorhabditis elegans embryo, some ventral
midline motoneurons extend a process circumferentially to the dorsal
midline and a process longitudinally along ventral nerve cord
interneurons. Circumferential migrations are guided by netrin UNC-6,
which repels motoneuron axons dorsally. Although the motoneuron cell
bodies and the longitudinal axons are positioned along UNC-6-expressing interneurons in the ventral nerve cord, the circumferential processes extend only from the motoneuron cell bodies and from defined locations along some longitudinal axons. This implies a mechanism regulates motoneuron branching of UNC-6-responsive processes. We show that expression of unc-6 C, which encodes UNC-6 without
domain C, partially rescues circumferential migration defects in
unc-6 null animals. This activity depends on the netrin
receptors UNC-5 and UNC-40. These results indicate that UNC-6C can
provide the circumferential guidance functions of UNC-6. Furthermore,
we show that expression of unc-6C causes motoneuron
branching and the extension of processes from abnormal positions along
the ventral nerve cord. This activity is also UNC-5- and
UNC-40-dependent. We propose that local interactions mediated by domain
C regulate motoneuron branching and responsiveness to the UNC-6 cue.
Key words:
netrin; UNC-6; C. elegans; guidance; axon
branching; genetics; invertebrate; in vivo
Copyright © 1999 Society for Neuroscience 0270-6474/99/19167048-09$05.00/0
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