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The Journal of Neuroscience, September 1, 1999, 19(17):7434-7449

Synaptic Control of Glycine and GABA_A Receptors and Gephyrin Expression in Cultured Motoneurons

Sabine Lévi¹, Dominique Chesnoy-Marchais², Werner Sieghart³, and Antoine Triller¹

¹ Laboratoire de Biologie Cellulaire de la Synapse Normale et Pathologique (Institut National de la Santé et de la Recherche Médicale U-497), ² Laboratoire de Neurobiologie (Centre National de la Recherche Scientifique URA-1867), Ecole Normale Supérieure, F-75005 Paris, France, and ³ University Clinic For Psychiatry, Department of Biochemical Psychiatry, A-1090 Vienna, Austria

We have evaluated the influence of the secretory phenotype of presynaptic boutons on the accumulation of postsynaptic glycine receptors (GlyRs), type A GABA receptors (GABA_ARs), and gephyrin clusters. The cellular distribution of these components was analyzed on motoneurons cultured either alone or with glycinergic and/or GABAergic neurons. In motoneurons cultured alone, we observed gephyrin clusters at nonsynaptic sites and in front of cholinergic boutons, whereas glycine and GABA_A receptors formed nonsynaptic clusters. These receptors are functionally and pharmacologically similar to those found in cultures of all spinal neurons. Motoneurons receiving GABAergic innervation from dorsal root ganglia neurons displayed postsynaptic clusters of gephyrin and GABA_AR but not of GlyR/ subunits. In motoneurons receiving glycinergic and GABAergic innervation from spinal interneurons, gephyrin, GlyR/, and GABA_AR formed mosaics at synaptic loci. These results indicate that (1) the transmitter phenotype of the presynaptic element determines the postsynaptic accumulation of specific receptors but not of gephyrin and (2) the postsynaptic accumulation of gephyrin alone cannot account for the formation of GlyR-rich microdomains.

Key words: dorsal root ganglia; GABA_A receptor; gephyrin; glycine receptor; motoneurons; picrotoxinin; presynaptic innervation; spinal neurons; strychnine

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Copyright © 1999 Society for Neuroscience  0270-6474/99/19177434-16$05.00/0

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