WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience
 QUICK SEARCH:   [advanced]


     
-


HOME
  |  
SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (64)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by David, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by David, G.

 Previous Article  |  Next Article 

The Journal of Neuroscience, September 1, 1999, 19(17):7495-7506

Mitochondrial Clearance of Cytosolic Ca2+ in Stimulated Lizard Motor Nerve Terminals Proceeds without Progressive Elevation of Mitochondrial Matrix [Ca2+]

Gavriel David

Department of Physiology and Biophysics, University of Miami School of Medicine, Miami Florida 33101

This study used fluorescent indicator dyes to measure changes in cytosolic and mitochondrial [Ca2+] produced by physiological stimulation of lizard motor nerve terminals. During repetitive action potential discharge at 10-50 Hz, the increase in average cytosolic [Ca2+] reached plateau at levels that increased with increasing stimulus frequency. This stabilization of cytosolic [Ca2+] was caused mainly by mitochondrial Ca2+ uptake, because drugs that depolarize mitochondria greatly increased the stimulation-induced elevation of cytosolic [Ca2+], whereas blockers of other Ca2+ clearance routes had little effect. Surprisingly, during this sustained Ca2+ uptake the free [Ca2+] in the mitochondrial matrix never exceeded a plateau level of ~1 µM, regardless of stimulation frequency or pattern. When stimulation ceased, matrix [Ca2+] decreased over a slow (~10 min) time course consisting of an initial plateau followed by a return to baseline. These measurements demonstrate that sustained mitochondrial Ca2+ uptake is not invariably accompanied by progressive elevation of matrix free [Ca2+]. Both the plateau of matrix free [Ca2+] during stimulation and its complex decay after stimulation could be accounted for by a model incorporating reversible formation of an insoluble Ca salt. This mechanism allows mitochondria to sequester large amounts of Ca2+ while maintaining matrix free [Ca2+] at levels sufficient to activate Ca2+-dependent mitochondrial dehydrogenases, but below levels that activate the permeability transition pore.

Key words: mitochondria; mitochondrial calcium uptake; presynaptic terminal; motor nerve terminal; mitochondrial matrix; calcium indicator dyes; calcium buffering; calcium sequestration


Copyright © 1999 Society for Neuroscience  0270-6474/99/19177495-12$05.00/0


This article has been cited by other articles:


Home page
J. Neurosci.Home page
M.-S. Kim and Y. M. Usachev
Mitochondrial Ca2+ Cycling Facilitates Activation of the Transcription Factor NFAT in Sensory Neurons
J. Neurosci., September 30, 2009; 29(39): 12101 - 12114.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
R. L. Pongratz, R. G. Kibbey, C. L. Kirkpatrick, X. Zhao, M. Pontoglio, M. Yaniv, C. B. Wollheim, G. I. Shulman, and G. W. Cline
Mitochondrial Dysfunction Contributes to Impaired Insulin Secretion in INS-1 Cells with Dominant-negative Mutations of HNF-1{alpha} and in HNF-1{alpha}-deficient Islets
J. Biol. Chem., June 19, 2009; 284(25): 16808 - 16821.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
K. T. Nguyen, L. E. Garcia-Chacon, J. N. Barrett, E. F. Barrett, and G. David
The {Psi}m depolarization that accompanies mitochondrial Ca2+ uptake is greater in mutant SOD1 than in wild-type mouse motor terminals
PNAS, February 10, 2009; 106(6): 2007 - 2011.
[Abstract] [Full Text] [PDF]


Home page
Hum Mol GenetHome page
J. Aydin, D. C. Andersson, S. L. Hanninen, A. Wredenberg, P. Tavi, C. B. Park, N.-G. Larsson, J. D. Bruton, and H. Westerblad
Increased mitochondrial Ca2+ and decreased sarcoplasmic reticulum Ca2+ in mitochondrial myopathy
Hum. Mol. Genet., January 15, 2009; 18(2): 278 - 288.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
Y. V. Medvedeva, M.-S. Kim, and Y. M. Usachev
Mechanisms of Prolonged Presynaptic Ca2+ Signaling and Glutamate Release Induced by TRPV1 Activation in Rat Sensory Neurons
J. Neurosci., May 14, 2008; 28(20): 5295 - 5311.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
D. Poburko, C.-H. Liao, V. S. Lemos, E. Lin, Y. Maruyama, W. C. Cole, and C. van Breemen
Transient Receptor Potential Channel 6 Mediated, Localized Cytosolic [Na+] Transients Drive Na+/Ca2+ Exchanger Mediated Ca2+ Entry in Purinergically Stimulated Aorta Smooth Muscle Cells
Circ. Res., November 9, 2007; 101(10): 1030 - 1038.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
J. Talbot, J. N. Barrett, E. F. Barrett, and G. David
Stimulation-induced changes in NADH fluorescence and mitochondrial membrane potential in lizard motor nerve terminals
J. Physiol., March 15, 2007; 579(3): 783 - 798.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Cell Physiol.Home page
M. Kuba, Y. Higure, H. Susaki, R. Hayato, and K. Kuba
Bidirectional Ca2+ coupling of mitochondria with the endoplasmic reticulum and regulation of multimodal Ca2+ entries in rat brown adipocytes
Am J Physiol Cell Physiol, February 1, 2007; 292(2): C896 - C908.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
L. E. Garcia-Chacon, K. T. Nguyen, G. David, and E. F. Barrett
Extrusion of Ca2+ from mouse motor terminal mitochondria via a Na+-Ca2+ exchanger increases post-tetanic evoked release
J. Physiol., August 1, 2006; 574(3): 663 - 675.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
M. A. Nikolaeva, B. Mukherjee, and P. K. Stys
Na+-Dependent Sources of Intra-Axonal Ca2+ Release in Rat Optic Nerve during In Vitro Chemical Ischemia
J. Neurosci., October 26, 2005; 25(43): 9960 - 9967.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
N. B. Pivovarova, H. V. Nguyen, C. A. Winters, C. A. Brantner, C. L. Smith, and S. B. Andrews
Excitotoxic Calcium Overload in a Subpopulation of Mitochondria Triggers Delayed Death in Hippocampal Neurons
J. Neurosci., June 16, 2004; 24(24): 5611 - 5622.
[Abstract] [Full Text] [PDF]


Home page
PhysiologyHome page
A. B. Parekh
Mitochondrial Regulation of Intracellular Ca2+ Signaling: More Than Just Simple Ca2+ Buffers
Physiology, December 1, 2003; 18(6): 252 - 256.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
F. Yang, X.-p. He, J. Russell, and B. Lu
Ca2+ influx-independent synaptic potentiation mediated by mitochondrial Na+-Ca2+ exchanger and protein kinase C
J. Cell Biol., November 10, 2003; 163(3): 511 - 523.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
G. Csordas and G. Hajnoczky
Plasticity of Mitochondrial Calcium Signaling
J. Biol. Chem., October 24, 2003; 278(43): 42273 - 42282.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
J. Bruton, P. Tavi, J. Aydin, H. Westerblad, and J. Lannergren
Mitochondrial and myoplasmic [Ca2+] in single fibres from mouse limb muscles during repeated tetanic contractions
J. Physiol., August 15, 2003; 551(1): 179 - 190.
[Abstract] [Full Text] [PDF]


Home page
J. Neurophysiol.Home page
J. D. Talbot, G. David, and E. F. Barrett
Inhibition of Mitochondrial Ca2+ Uptake Affects Phasic Release From Motor Terminals Differently Depending on External [Ca2+]
J Neurophysiol, July 1, 2003; 90(1): 491 - 502.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
L. Vila, E. F Barrett, and J. N Barrett
Stimulation-induced mitochondrial [Ca2+] elevations in mouse motor terminals: comparison of wild-type with SOD1-G93A
J. Physiol., June 15, 2003; 549(3): 719 - 728.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. Chalmers and D. G. Nicholls
The Relationship between Free and Total Calcium Concentrations in the Matrix of Liver and Brain Mitochondria
J. Biol. Chem., May 23, 2003; 278(21): 19062 - 19070.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
G. David and E. F Barrett
Mitochondrial Ca2+ uptake prevents desynchronization of quantal release and minimizes depletion during repetitive stimulation of mouse motor nerve terminals
J. Physiol., April 15, 2003; 548(2): 425 - 438.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
N. B. Pivovarova, L. D. Pozzo-Miller, J. Hongpaisan, and S. B. Andrews
Correlated Calcium Uptake and Release by Mitochondria and Endoplasmic Reticulum of CA3 Hippocampal Dendrites after Afferent Synaptic Stimulation
J. Neurosci., December 15, 2002; 22(24): 10653 - 10661.
[Abstract] [Full Text] [PDF]


Home page
J. Neurophysiol.Home page
R. Kovacs, S. Schuchmann, S. Gabriel, O. Kann, J. Kardos, and U. Heinemann
Free Radical-Mediated Cell Damage After Experimental Status Epilepticus in Hippocampal Slice Cultures
J Neurophysiol, December 1, 2002; 88(6): 2909 - 2918.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
B. Billups and I. D. Forsythe
Presynaptic Mitochondrial Calcium Sequestration Influences Transmission at Mammalian Central Synapses
J. Neurosci., July 15, 2002; 22(14): 5840 - 5847.
[Abstract] [Full Text] [PDF]


Home page
J. Neurophysiol.Home page
S. Suzuki, M. Osanai, N. Mitsumoto, T. Akita, K. Narita, H. Kijima, and K. Kuba
Ca2+-Dependent Ca2+ Clearance Via Mitochondrial Uptake and Plasmalemmal Extrusion in Frog Motor Nerve Terminals
J Neurophysiol, April 1, 2002; 87(4): 1816 - 1823.
[Abstract] [Full Text] [PDF]


Home page
J. Neurophysiol.Home page
G. J. Wang and S. A. Thayer
NMDA-Induced Calcium Loads Recycle Across the Mitochondrial Inner Membrane of Hippocampal Neurons in Culture
J Neurophysiol, February 1, 2002; 87(2): 740 - 749.
[Abstract] [Full Text] [PDF]


Home page
JGPHome page
E. F. Barrett
Contrasting Contributions of Endoplasmic Reticulum and Mitochondria to Ca2+ Handling in Neurons
J. Gen. Physiol., July 1, 2001; 118(1): 79 - 82.
[Full Text] [PDF]


Home page
JGPHome page
J. Hongpaisan, N. B. Pivovarova, S. L. Colegrove, R. D. Leapman, D. D. Friel, and S. B. Andrews
Multiple Modes of Calcium-Induced Calcium Release in Sympathetic Neurons II: A [Ca2+]i- and Location-Dependent Transition from Endoplasmic Reticulum Ca Accumulation to Net Ca Release
J. Gen. Physiol., July 1, 2001; 118(1): 101 - 112.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
M. A Calupca, C. Prior, L. A Merriam, G. M Hendricks, and R. L Parsons
Presynaptic function is altered in snake K+-depolarized motor nerve terminals containing compromised mitochondria
J. Physiol., April 1, 2001; 532(1): 217 - 227.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
C. W. Tsang, D. B. Elrick, and M. P. Charlton
alpha -Latrotoxin Releases Calcium in Frog Motor Nerve Terminals
J. Neurosci., December 1, 2000; 20(23): 8685 - 8692.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
P. Ronde, J. J Dougherty, and R. A Nichols
Functional IP3- and ryanodine-sensitive calcium stores in presynaptic varicosities of NG108-15 (rodent neuroblastoma x glioma hybrid) cells
J. Physiol., December 1, 2000; 529(2): 307 - 319.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
M. R Duchen
Mitochondria and calcium: from cell signalling to cell death
J. Physiol., November 15, 2000; 529(1): 57 - 68.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
G. Hajnoczky, G. Csordas, M. Madesh, and P. Pacher
The machinery of local Ca2+ signalling between sarco-endoplasmic reticulum and mitochondria
J. Physiol., November 15, 2000; 529(1): 69 - 81.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
G. David and E. F. Barrett
Stimulation-Evoked Increases in Cytosolic [Ca2+] in Mouse Motor Nerve Terminals Are Limited by Mitochondrial Uptake and Are Temperature-Dependent
J. Neurosci., October 1, 2000; 20(19): 7290 - 7296.
[Abstract] [Full Text] [PDF]


Home page
JGPHome page
S. L. Colegrove, M. A. Albrecht, and D. D. Friel
Quantitative Analysis of Mitochondrial Ca2+ Uptake and Release Pathways in Sympathetic Neurons: Reconstruction of the Recovery after Depolarization-Evoked [Ca2+]i Elevations
J. Gen. Physiol., March 1, 2000; 115(3): 371 - 388.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
E. J. Kaftan, T. Xu, R. F. Abercrombie, and B. Hille
Mitochondria Shape Hormonally Induced Cytoplasmic Calcium Oscillations and Modulate Exocytosis
J. Biol. Chem., August 11, 2000; 275(33): 25465 - 25470.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
T. J. Collins, P. Lipp, M. J. Berridge, and M. D. Bootman
Mitochondrial Ca2+ Uptake Depends on the Spatial and Temporal Profile of Cytosolic Ca2+ Signals
J. Biol. Chem., July 6, 2001; 276(28): 26411 - 26420.
[Abstract] [Full Text] [PDF]



-
-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help

-
Copyright 2009 by Society for Neuroscience ONLINE ISSN: 1529-2401
-