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The Journal of Neuroscience, September 15, 1999, 19(18):7742-7756
Two Types of K+ Channel Subunit, Erg1 and KCNQ2/3,
Contribute to the M-Like Current in a Mammalian Neuronal Cell
A. A.
Selyanko1,
J. K.
Hadley1,
I. C.
Wood2,
F. C.
Abogadie2,
P.
Delmas2,
N. J.
Buckley2,
B.
London3, and
D. A.
Brown1
1 Department of Pharmacology, 2 Wellcome
Laboratory for Molecular Pharmacology, University College London,
London, WC1E 6BT, United Kingdom, and 3 Cardiovascular
Institute, University of Pittsburgh Medical Center, Pittsburgh,
Pennsylvania 15213
The potassium M current was originally identified in sympathetic
ganglion cells, and analogous currents have been reported in some
central neurons and also in some neural cell lines. It has recently
been suggested that the M channel in sympathetic neurons comprises a
heteromultimer of KCNQ2 and KCNQ3 (Wang
et al., 1998) but it is unclear whether all other M-like currents are
generated by these channels. Here we report that the M-like current
previously described in NG108-15 mouse neuroblastoma x rat glioma
cells has two components, "fast" and "slow", that may be
differentiated kinetically and pharmacologically. We provide evidence
from PCR analysis and expression studies to indicate that these two
components are mediated by two distinct molecular species of
K+ channel: the fast component resembles that
in sympathetic ganglia and is probably carried by
KCNQ2/3 channels, whereas the slow component appears to
be carried by merg1a channels. Thus, the channels generating M-like
currents in different cells may be heterogeneous in molecular composition.
Key words:
potassium channels; neuroblastoma x glioma hybrid cells; M current; sympathetic neuron; Erg1; KCNQ
Copyright © 1999 Society for Neuroscience 0270-6474/99/19187742-15$05.00/0
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